Limits...
Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.Finally, reallocation of membrane lipids and induction of de novo triacylglycerol biosynthesis directed cells to accumulation of neutral lipids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588885&req=5

Figure 1: Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.

Mentions: P. tricornutum cell growth was monitored daily in both cultures. All N-replete cultures remained in the exponential phase throughout the time course of the experiment. During the experiment period, cell density increased from 5×104 to 1.92×106 cells ml–1 in replete cultures (Fig. 1A). N-free cultures showed similar cell growth compared with N-replete cultures until 48h, but significantly lower growth at 72h, with a cell density of 1.02×106 cells ml–1. In order to compare physiological and transcriptional responses before and after the N deprivation started to affect cell growth, the time points of 48 and 72h were chosen for further physiological and molecular experiments.


Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588885&req=5

Figure 1: Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.
Mentions: P. tricornutum cell growth was monitored daily in both cultures. All N-replete cultures remained in the exponential phase throughout the time course of the experiment. During the experiment period, cell density increased from 5×104 to 1.92×106 cells ml–1 in replete cultures (Fig. 1A). N-free cultures showed similar cell growth compared with N-replete cultures until 48h, but significantly lower growth at 72h, with a cell density of 1.02×106 cells ml–1. In order to compare physiological and transcriptional responses before and after the N deprivation started to affect cell growth, the time points of 48 and 72h were chosen for further physiological and molecular experiments.

Bottom Line: Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.Finally, reallocation of membrane lipids and induction of de novo triacylglycerol biosynthesis directed cells to accumulation of neutral lipids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus