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Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures.Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.
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Figure 1: Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.

Mentions: P. tricornutum cell growth was monitored daily in both cultures. All N-replete cultures remained in the exponential phase throughout the time course of the experiment. During the experiment period, cell density increased from 5×104 to 1.92×106 cells ml–1 in replete cultures (Fig. 1A). N-free cultures showed similar cell growth compared with N-replete cultures until 48h, but significantly lower growth at 72h, with a cell density of 1.02×106 cells ml–1. In order to compare physiological and transcriptional responses before and after the N deprivation started to affect cell growth, the time points of 48 and 72h were chosen for further physiological and molecular experiments.


Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum.

Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T - J. Exp. Bot. (2015)

Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588885&req=5

Figure 1: Physiological responses of P. tricornutum to nitrate deprivation. Growth curves (A) and changes in maximum quantum yield (Fv/Fm) (B) of P. tricornutum in N-replete (f/2 medium) and N-deprived (f/2 medium minus nitrate) cultures. Arrows indicate sampling time points. Values are means±standard deviation of four biological replicates.
Mentions: P. tricornutum cell growth was monitored daily in both cultures. All N-replete cultures remained in the exponential phase throughout the time course of the experiment. During the experiment period, cell density increased from 5×104 to 1.92×106 cells ml–1 in replete cultures (Fig. 1A). N-free cultures showed similar cell growth compared with N-replete cultures until 48h, but significantly lower growth at 72h, with a cell density of 1.02×106 cells ml–1. In order to compare physiological and transcriptional responses before and after the N deprivation started to affect cell growth, the time points of 48 and 72h were chosen for further physiological and molecular experiments.

Bottom Line: Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures.Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level.The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.