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Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.

Royo B, Moran JF, Ratcliffe RG, Gupta KJ - J. Exp. Bot. (2015)

Bottom Line: The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia.Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype.It is concluded that NO is required for the induction of the AOX pathway when seedlings are grown under phosphate-limiting conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of Oxford, South Park Road, Oxford OX1 3RB, UK Institute of Agrobiotechnology, IdAB-CSIC-Public University of Navarre-Government of Navarre, Avda. Pamplona 123, E-31192, Mutilva, Navarre, Spain.

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Effect of Pi supply on the AOX level in Arabidopsis seedlings. (A) Detection of AOX1A by immunoblotting in the mitochondrial protein fraction from 10-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi. (B) Bands were quantified using Image J software (n=2).
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Figure 4: Effect of Pi supply on the AOX level in Arabidopsis seedlings. (A) Detection of AOX1A by immunoblotting in the mitochondrial protein fraction from 10-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi. (B) Bands were quantified using Image J software (n=2).

Mentions: There was a substantial increase in the AOX level in WT plants grown on 0mM Pi (Fig. 4), which correlated with the increased capacity of the AOX pathway and the effect of SHAM on the respiration rate of the KCN-treated seedlings (Fig. 3A, C). However Pi deprivation had no effect on the AOX protein level in the nia mutant (Fig. 4), suggesting that the induction of AOX under low Pi required an increase in NO.


Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.

Royo B, Moran JF, Ratcliffe RG, Gupta KJ - J. Exp. Bot. (2015)

Effect of Pi supply on the AOX level in Arabidopsis seedlings. (A) Detection of AOX1A by immunoblotting in the mitochondrial protein fraction from 10-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi. (B) Bands were quantified using Image J software (n=2).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588884&req=5

Figure 4: Effect of Pi supply on the AOX level in Arabidopsis seedlings. (A) Detection of AOX1A by immunoblotting in the mitochondrial protein fraction from 10-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi. (B) Bands were quantified using Image J software (n=2).
Mentions: There was a substantial increase in the AOX level in WT plants grown on 0mM Pi (Fig. 4), which correlated with the increased capacity of the AOX pathway and the effect of SHAM on the respiration rate of the KCN-treated seedlings (Fig. 3A, C). However Pi deprivation had no effect on the AOX protein level in the nia mutant (Fig. 4), suggesting that the induction of AOX under low Pi required an increase in NO.

Bottom Line: The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia.Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype.It is concluded that NO is required for the induction of the AOX pathway when seedlings are grown under phosphate-limiting conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of Oxford, South Park Road, Oxford OX1 3RB, UK Institute of Agrobiotechnology, IdAB-CSIC-Public University of Navarre-Government of Navarre, Avda. Pamplona 123, E-31192, Mutilva, Navarre, Spain.

No MeSH data available.


Related in: MedlinePlus