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Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.

Royo B, Moran JF, Ratcliffe RG, Gupta KJ - J. Exp. Bot. (2015)

Bottom Line: The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia.Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype.It is concluded that NO is required for the induction of the AOX pathway when seedlings are grown under phosphate-limiting conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of Oxford, South Park Road, Oxford OX1 3RB, UK Institute of Agrobiotechnology, IdAB-CSIC-Public University of Navarre-Government of Navarre, Avda. Pamplona 123, E-31192, Mutilva, Navarre, Spain.

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Related in: MedlinePlus

Effect of Pi supply on the NO level in Arabidopsis roots. NO was quantified in the roots of 14-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi by: (A) DAF-2DA fluorescence; and (B) a gas phase Griess reagent assay. Means (n=3) with different letters are significantly different (one-way ANOVA, P< 0.05).
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Figure 2: Effect of Pi supply on the NO level in Arabidopsis roots. NO was quantified in the roots of 14-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi by: (A) DAF-2DA fluorescence; and (B) a gas phase Griess reagent assay. Means (n=3) with different letters are significantly different (one-way ANOVA, P< 0.05).

Mentions: The effect of low Pi on NO production was measured using the fluorophore DAF-2DA. The advantage of this cell-permeant dye is that it diffuses to NO producing sites and reacts with NO to form a highly fluorescent product. WT roots had higher levels of NO than the nia mutant when the seedlings were grown on 1mM Pi, but while the NO level increased substantially in the WT roots grown on 0mM Pi, the level decreased slightly in the roots of the nia mutant (Fig. 2A; Supplementary Fig. S3). Similar results were obtained when NO production was analysed with the gas phase Griess reagent assay. These measurements showed that the rate of NO production increased substantially in WT roots grown on 0mM Pi, whereas there was no change in the roots of the nia mutant (Fig. 2B). It is good practice to measure NO by more than one method (Gupta and Igamberdiev, 2013) and here the two assays show that Pi deprivation increased the capacity for NO production and the endogenous NO level in Arabidopsis roots.


Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.

Royo B, Moran JF, Ratcliffe RG, Gupta KJ - J. Exp. Bot. (2015)

Effect of Pi supply on the NO level in Arabidopsis roots. NO was quantified in the roots of 14-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi by: (A) DAF-2DA fluorescence; and (B) a gas phase Griess reagent assay. Means (n=3) with different letters are significantly different (one-way ANOVA, P< 0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588884&req=5

Figure 2: Effect of Pi supply on the NO level in Arabidopsis roots. NO was quantified in the roots of 14-d-old WT and nia seedlings grown on a medium containing 0 or 1mM Pi by: (A) DAF-2DA fluorescence; and (B) a gas phase Griess reagent assay. Means (n=3) with different letters are significantly different (one-way ANOVA, P< 0.05).
Mentions: The effect of low Pi on NO production was measured using the fluorophore DAF-2DA. The advantage of this cell-permeant dye is that it diffuses to NO producing sites and reacts with NO to form a highly fluorescent product. WT roots had higher levels of NO than the nia mutant when the seedlings were grown on 1mM Pi, but while the NO level increased substantially in the WT roots grown on 0mM Pi, the level decreased slightly in the roots of the nia mutant (Fig. 2A; Supplementary Fig. S3). Similar results were obtained when NO production was analysed with the gas phase Griess reagent assay. These measurements showed that the rate of NO production increased substantially in WT roots grown on 0mM Pi, whereas there was no change in the roots of the nia mutant (Fig. 2B). It is good practice to measure NO by more than one method (Gupta and Igamberdiev, 2013) and here the two assays show that Pi deprivation increased the capacity for NO production and the endogenous NO level in Arabidopsis roots.

Bottom Line: The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia.Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype.It is concluded that NO is required for the induction of the AOX pathway when seedlings are grown under phosphate-limiting conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of Oxford, South Park Road, Oxford OX1 3RB, UK Institute of Agrobiotechnology, IdAB-CSIC-Public University of Navarre-Government of Navarre, Avda. Pamplona 123, E-31192, Mutilva, Navarre, Spain.

No MeSH data available.


Related in: MedlinePlus