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The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis.

Jeon Y, Park YJ, Cho HK, Jung HJ, Ahn TK, Kang H, Pai HS - J. Exp. Bot. (2015)

Bottom Line: Depletion of NSN1 delayed 25S rRNA maturation and biogenesis of the 60S ribosome subunit, and repressed global translation.NSN1-deficient plants exhibited premature leaf senescence, excessive accumulation of reactive oxygen species, and senescence-related gene expression.Taken together, these results suggest that NSN1 plays a crucial role in plant growth and senescence by modulating ribosome biogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Systems Biology, Yonsei University, Seoul 120-749, Korea.

No MeSH data available.


Related in: MedlinePlus

Delayed rRNA processing and ribosome biogenesis in NSN1-deficient Arabidopsis plants. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (A) Metabolic rRNA labelling. Seedlings were labelled with [α-32P]UTP. Total RNA extracted from the seedlings was separated by agarose gel electrophoresis and analysed with a phosphorimager. Newly synthesized mature 25S and 18S rRNAs are marked. (B) Relative 25S/18S rRNA ratio. Band intensities of 25S rRNA and 18S rRNA in the samples shown in (A) are compared. (C) Absorbance profiles of ribosomes at 254nm. Ribosomes were purified from seedlings using ultracentrifugation on a sucrose density gradient.
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Figure 7: Delayed rRNA processing and ribosome biogenesis in NSN1-deficient Arabidopsis plants. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (A) Metabolic rRNA labelling. Seedlings were labelled with [α-32P]UTP. Total RNA extracted from the seedlings was separated by agarose gel electrophoresis and analysed with a phosphorimager. Newly synthesized mature 25S and 18S rRNAs are marked. (B) Relative 25S/18S rRNA ratio. Band intensities of 25S rRNA and 18S rRNA in the samples shown in (A) are compared. (C) Absorbance profiles of ribosomes at 254nm. Ribosomes were purified from seedlings using ultracentrifugation on a sucrose density gradient.

Mentions: The effect of NSN1 depletion on nascent rRNA synthesis was examined using in vivo [α-32P]UTP labelling (Fig. 7A). DEX-inducible NSN1 RNAi Arabidopsis seedlings were grown in media with ethanol (–DEX) or with DEX for 7–8 d. After incorporation of [α-32P]UTP into nascent RNA transcripts, total RNA was purified and separated by agarose gel electrophoresis. Two main radioactive bands were detected, which represented newly synthesized 25S and 18S rRNAs. The ratio of 25S rRNA to 18S rRNA was calculated based on the band intensities of rRNAs (Fig. 7B). The 25S/18S rRNA ratios suggest that nascent synthesis of mature 25S rRNA was significantly reduced in DEX-treated NSN1 RNAi samples (#4 and #17). This result is consistent with delayed 25S rRNA processing in the NSN1-deficient nucleolus.


The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis.

Jeon Y, Park YJ, Cho HK, Jung HJ, Ahn TK, Kang H, Pai HS - J. Exp. Bot. (2015)

Delayed rRNA processing and ribosome biogenesis in NSN1-deficient Arabidopsis plants. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (A) Metabolic rRNA labelling. Seedlings were labelled with [α-32P]UTP. Total RNA extracted from the seedlings was separated by agarose gel electrophoresis and analysed with a phosphorimager. Newly synthesized mature 25S and 18S rRNAs are marked. (B) Relative 25S/18S rRNA ratio. Band intensities of 25S rRNA and 18S rRNA in the samples shown in (A) are compared. (C) Absorbance profiles of ribosomes at 254nm. Ribosomes were purified from seedlings using ultracentrifugation on a sucrose density gradient.
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Related In: Results  -  Collection

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Figure 7: Delayed rRNA processing and ribosome biogenesis in NSN1-deficient Arabidopsis plants. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (A) Metabolic rRNA labelling. Seedlings were labelled with [α-32P]UTP. Total RNA extracted from the seedlings was separated by agarose gel electrophoresis and analysed with a phosphorimager. Newly synthesized mature 25S and 18S rRNAs are marked. (B) Relative 25S/18S rRNA ratio. Band intensities of 25S rRNA and 18S rRNA in the samples shown in (A) are compared. (C) Absorbance profiles of ribosomes at 254nm. Ribosomes were purified from seedlings using ultracentrifugation on a sucrose density gradient.
Mentions: The effect of NSN1 depletion on nascent rRNA synthesis was examined using in vivo [α-32P]UTP labelling (Fig. 7A). DEX-inducible NSN1 RNAi Arabidopsis seedlings were grown in media with ethanol (–DEX) or with DEX for 7–8 d. After incorporation of [α-32P]UTP into nascent RNA transcripts, total RNA was purified and separated by agarose gel electrophoresis. Two main radioactive bands were detected, which represented newly synthesized 25S and 18S rRNAs. The ratio of 25S rRNA to 18S rRNA was calculated based on the band intensities of rRNAs (Fig. 7B). The 25S/18S rRNA ratios suggest that nascent synthesis of mature 25S rRNA was significantly reduced in DEX-treated NSN1 RNAi samples (#4 and #17). This result is consistent with delayed 25S rRNA processing in the NSN1-deficient nucleolus.

Bottom Line: Depletion of NSN1 delayed 25S rRNA maturation and biogenesis of the 60S ribosome subunit, and repressed global translation.NSN1-deficient plants exhibited premature leaf senescence, excessive accumulation of reactive oxygen species, and senescence-related gene expression.Taken together, these results suggest that NSN1 plays a crucial role in plant growth and senescence by modulating ribosome biogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Systems Biology, Yonsei University, Seoul 120-749, Korea.

No MeSH data available.


Related in: MedlinePlus