Limits...
The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis.

Jeon Y, Park YJ, Cho HK, Jung HJ, Ahn TK, Kang H, Pai HS - J. Exp. Bot. (2015)

Bottom Line: Depletion of NSN1 delayed 25S rRNA maturation and biogenesis of the 60S ribosome subunit, and repressed global translation.NSN1-deficient plants exhibited premature leaf senescence, excessive accumulation of reactive oxygen species, and senescence-related gene expression.Taken together, these results suggest that NSN1 plays a crucial role in plant growth and senescence by modulating ribosome biogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Systems Biology, Yonsei University, Seoul 120-749, Korea.

No MeSH data available.


Related in: MedlinePlus

Reduced global translation in NSN1-deficient plants. (A) [35S]Methionine labelling. After [35S]methionine labelling of Arabidopsis RNAi seedlings, total protein extracts were separated by SDS–PAGE, and the gels were dried and analysed with a phosphorimager. A duplicate gel was stained with Coomassie blue to show the Rubisco large subunit (rbcL) as loading control. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (B) Polysome loading of RPL10a. Sucrose density gradient fractions from N. benthamiana TRV, TRV:NbNSN1(N), and TRV:EBP2(N) VIGS plants (14 DAI) were analysed by immunoblotting with anti-RPL10a antibody. The fourth to fifth leaf above the infiltrated leaf was used for the analysis. Positions of polysomes are indicated. Lanes 1–10 indicate the gradient fractions from the top (15%) to the bottom (55%).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588883&req=5

Figure 6: Reduced global translation in NSN1-deficient plants. (A) [35S]Methionine labelling. After [35S]methionine labelling of Arabidopsis RNAi seedlings, total protein extracts were separated by SDS–PAGE, and the gels were dried and analysed with a phosphorimager. A duplicate gel was stained with Coomassie blue to show the Rubisco large subunit (rbcL) as loading control. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (B) Polysome loading of RPL10a. Sucrose density gradient fractions from N. benthamiana TRV, TRV:NbNSN1(N), and TRV:EBP2(N) VIGS plants (14 DAI) were analysed by immunoblotting with anti-RPL10a antibody. The fourth to fifth leaf above the infiltrated leaf was used for the analysis. Positions of polysomes are indicated. Lanes 1–10 indicate the gradient fractions from the top (15%) to the bottom (55%).

Mentions: Cellular protein translation activity was examined using [35S]methionine labelling in WT Arabidopsis and the DEX-inducible NSN1 RNAi (#4 and #17) lines (Fig. 6A). Seedlings were grown on media with or without DEX for 7–8 d, briefly incubated with [35S]methionine, and proteins were extracted. The radioactive protein profiles were obtained using SDS–PAGE of the protein extracts and phosphorimager analysis. Nascent protein synthesis in DEX-treated NSN1 RNAi (#4 and #17) lines was reduced compared with (–)DEX RNAi seedlings or DEX-treated WT seedlings. This result suggests that NSN1 deficiency leads to a reduction in protein synthesis (Fig. 6A).


The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis.

Jeon Y, Park YJ, Cho HK, Jung HJ, Ahn TK, Kang H, Pai HS - J. Exp. Bot. (2015)

Reduced global translation in NSN1-deficient plants. (A) [35S]Methionine labelling. After [35S]methionine labelling of Arabidopsis RNAi seedlings, total protein extracts were separated by SDS–PAGE, and the gels were dried and analysed with a phosphorimager. A duplicate gel was stained with Coomassie blue to show the Rubisco large subunit (rbcL) as loading control. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (B) Polysome loading of RPL10a. Sucrose density gradient fractions from N. benthamiana TRV, TRV:NbNSN1(N), and TRV:EBP2(N) VIGS plants (14 DAI) were analysed by immunoblotting with anti-RPL10a antibody. The fourth to fifth leaf above the infiltrated leaf was used for the analysis. Positions of polysomes are indicated. Lanes 1–10 indicate the gradient fractions from the top (15%) to the bottom (55%).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588883&req=5

Figure 6: Reduced global translation in NSN1-deficient plants. (A) [35S]Methionine labelling. After [35S]methionine labelling of Arabidopsis RNAi seedlings, total protein extracts were separated by SDS–PAGE, and the gels were dried and analysed with a phosphorimager. A duplicate gel was stained with Coomassie blue to show the Rubisco large subunit (rbcL) as loading control. Whole seedlings grown for 7–8 d on (–)DEX or (+)DEX media were used for the analyses. (B) Polysome loading of RPL10a. Sucrose density gradient fractions from N. benthamiana TRV, TRV:NbNSN1(N), and TRV:EBP2(N) VIGS plants (14 DAI) were analysed by immunoblotting with anti-RPL10a antibody. The fourth to fifth leaf above the infiltrated leaf was used for the analysis. Positions of polysomes are indicated. Lanes 1–10 indicate the gradient fractions from the top (15%) to the bottom (55%).
Mentions: Cellular protein translation activity was examined using [35S]methionine labelling in WT Arabidopsis and the DEX-inducible NSN1 RNAi (#4 and #17) lines (Fig. 6A). Seedlings were grown on media with or without DEX for 7–8 d, briefly incubated with [35S]methionine, and proteins were extracted. The radioactive protein profiles were obtained using SDS–PAGE of the protein extracts and phosphorimager analysis. Nascent protein synthesis in DEX-treated NSN1 RNAi (#4 and #17) lines was reduced compared with (–)DEX RNAi seedlings or DEX-treated WT seedlings. This result suggests that NSN1 deficiency leads to a reduction in protein synthesis (Fig. 6A).

Bottom Line: Depletion of NSN1 delayed 25S rRNA maturation and biogenesis of the 60S ribosome subunit, and repressed global translation.NSN1-deficient plants exhibited premature leaf senescence, excessive accumulation of reactive oxygen species, and senescence-related gene expression.Taken together, these results suggest that NSN1 plays a crucial role in plant growth and senescence by modulating ribosome biogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Systems Biology, Yonsei University, Seoul 120-749, Korea.

No MeSH data available.


Related in: MedlinePlus