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Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment.

Fister AS, O'Neil ST, Shi Z, Zhang Y, Tyler BM, Guiltinan MJ, Maximova SN - J. Exp. Bot. (2015)

Bottom Line: Varietal differences in defence responses provide insights into the mechanisms of resistance and are a key resource for plant breeders.Transient overexpression of TcNPR1, a major transcriptional regulator of the SA-dependent plant immune system, also increased pathogen tolerance in cacao leaves.These experiments revealed critical insights regarding the molecular differences between cacao varieties, which will allow a better understanding of defence mechanisms to help guide breeding programmes.

View Article: PubMed Central - PubMed

Affiliation: The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

No MeSH data available.


Related in: MedlinePlus

NBT and DAB staining of cacao leaf discs 24h after SA or water treatment. (A) NBT-stained ICS1 treated with water, (B) NBT-stained Sca6 treated with water, (C) NBT-stained ICS1 treated with SA, (D) NBT-stained Sca6 treated with SA, (E) DAB-stained ICS1 treated with water, (F) DAB-stained Sca6 treated with water, (G) DAB-stained ICS1 treated with SA, (H) DAB-stained Sca6 treated with SA. Scale bars, 1cm. (I) Mean product area of leaf disc stained by NBT and mean grey value of stained area for each genotype and treatment. (J) Mean product of area stained by DAB and mean grey value of stained area for each genotype and treatment. In I and J, differences between bars marked with the same letter are not statistically significant (Fisher’s PLSD analysis P>0.05), standard errors were calculated from five biological replicates, and each biological replicate is a plate containing three leaf discs.
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Figure 7: NBT and DAB staining of cacao leaf discs 24h after SA or water treatment. (A) NBT-stained ICS1 treated with water, (B) NBT-stained Sca6 treated with water, (C) NBT-stained ICS1 treated with SA, (D) NBT-stained Sca6 treated with SA, (E) DAB-stained ICS1 treated with water, (F) DAB-stained Sca6 treated with water, (G) DAB-stained ICS1 treated with SA, (H) DAB-stained Sca6 treated with SA. Scale bars, 1cm. (I) Mean product area of leaf disc stained by NBT and mean grey value of stained area for each genotype and treatment. (J) Mean product of area stained by DAB and mean grey value of stained area for each genotype and treatment. In I and J, differences between bars marked with the same letter are not statistically significant (Fisher’s PLSD analysis P>0.05), standard errors were calculated from five biological replicates, and each biological replicate is a plate containing three leaf discs.


Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment.

Fister AS, O'Neil ST, Shi Z, Zhang Y, Tyler BM, Guiltinan MJ, Maximova SN - J. Exp. Bot. (2015)

NBT and DAB staining of cacao leaf discs 24h after SA or water treatment. (A) NBT-stained ICS1 treated with water, (B) NBT-stained Sca6 treated with water, (C) NBT-stained ICS1 treated with SA, (D) NBT-stained Sca6 treated with SA, (E) DAB-stained ICS1 treated with water, (F) DAB-stained Sca6 treated with water, (G) DAB-stained ICS1 treated with SA, (H) DAB-stained Sca6 treated with SA. Scale bars, 1cm. (I) Mean product area of leaf disc stained by NBT and mean grey value of stained area for each genotype and treatment. (J) Mean product of area stained by DAB and mean grey value of stained area for each genotype and treatment. In I and J, differences between bars marked with the same letter are not statistically significant (Fisher’s PLSD analysis P>0.05), standard errors were calculated from five biological replicates, and each biological replicate is a plate containing three leaf discs.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588882&req=5

Figure 7: NBT and DAB staining of cacao leaf discs 24h after SA or water treatment. (A) NBT-stained ICS1 treated with water, (B) NBT-stained Sca6 treated with water, (C) NBT-stained ICS1 treated with SA, (D) NBT-stained Sca6 treated with SA, (E) DAB-stained ICS1 treated with water, (F) DAB-stained Sca6 treated with water, (G) DAB-stained ICS1 treated with SA, (H) DAB-stained Sca6 treated with SA. Scale bars, 1cm. (I) Mean product area of leaf disc stained by NBT and mean grey value of stained area for each genotype and treatment. (J) Mean product of area stained by DAB and mean grey value of stained area for each genotype and treatment. In I and J, differences between bars marked with the same letter are not statistically significant (Fisher’s PLSD analysis P>0.05), standard errors were calculated from five biological replicates, and each biological replicate is a plate containing three leaf discs.
Bottom Line: Varietal differences in defence responses provide insights into the mechanisms of resistance and are a key resource for plant breeders.Transient overexpression of TcNPR1, a major transcriptional regulator of the SA-dependent plant immune system, also increased pathogen tolerance in cacao leaves.These experiments revealed critical insights regarding the molecular differences between cacao varieties, which will allow a better understanding of defence mechanisms to help guide breeding programmes.

View Article: PubMed Central - PubMed

Affiliation: The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

No MeSH data available.


Related in: MedlinePlus