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Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment.

Fister AS, O'Neil ST, Shi Z, Zhang Y, Tyler BM, Guiltinan MJ, Maximova SN - J. Exp. Bot. (2015)

Bottom Line: Varietal differences in defence responses provide insights into the mechanisms of resistance and are a key resource for plant breeders.Transient overexpression of TcNPR1, a major transcriptional regulator of the SA-dependent plant immune system, also increased pathogen tolerance in cacao leaves.These experiments revealed critical insights regarding the molecular differences between cacao varieties, which will allow a better understanding of defence mechanisms to help guide breeding programmes.

View Article: PubMed Central - PubMed

Affiliation: The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

No MeSH data available.


Related in: MedlinePlus

Functional analysis of TcNPR1. (A) Representative images of lesions from control and TcNPR1 transiently transformed leaves 2 d after Phytophthora tropicalis inoculation. (B) qRT-PCR analysis of TcNPR1 transcript 2 d after vacuum infiltration. Ctrl, control; NPR1, TcNPR1overexpression. Bars represent means ±SE of three biological replicates. (C) Average lesion areas from control and TcNPR1 overexpressing leaves were measured 3 d after inoculation using ImageJ. Bars represent the means ±SE of measurements from 12 lesion spots from four leaf discs of each genotype. The asterisk denotes a significant difference determined by single factor ANOVA (P<0.05). (D) Pathogen biomass was measured at the lesion sites by qPCR to determine the ratio of pathogen DNA to cacao DNA 2 d after inoculation. Bars represent four biological replicates, each with three technical replicates. The asterisk denotes a significant difference determined by single factor ANOVA analysis (P<0.05).
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Figure 2: Functional analysis of TcNPR1. (A) Representative images of lesions from control and TcNPR1 transiently transformed leaves 2 d after Phytophthora tropicalis inoculation. (B) qRT-PCR analysis of TcNPR1 transcript 2 d after vacuum infiltration. Ctrl, control; NPR1, TcNPR1overexpression. Bars represent means ±SE of three biological replicates. (C) Average lesion areas from control and TcNPR1 overexpressing leaves were measured 3 d after inoculation using ImageJ. Bars represent the means ±SE of measurements from 12 lesion spots from four leaf discs of each genotype. The asterisk denotes a significant difference determined by single factor ANOVA (P<0.05). (D) Pathogen biomass was measured at the lesion sites by qPCR to determine the ratio of pathogen DNA to cacao DNA 2 d after inoculation. Bars represent four biological replicates, each with three technical replicates. The asterisk denotes a significant difference determined by single factor ANOVA analysis (P<0.05).


Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment.

Fister AS, O'Neil ST, Shi Z, Zhang Y, Tyler BM, Guiltinan MJ, Maximova SN - J. Exp. Bot. (2015)

Functional analysis of TcNPR1. (A) Representative images of lesions from control and TcNPR1 transiently transformed leaves 2 d after Phytophthora tropicalis inoculation. (B) qRT-PCR analysis of TcNPR1 transcript 2 d after vacuum infiltration. Ctrl, control; NPR1, TcNPR1overexpression. Bars represent means ±SE of three biological replicates. (C) Average lesion areas from control and TcNPR1 overexpressing leaves were measured 3 d after inoculation using ImageJ. Bars represent the means ±SE of measurements from 12 lesion spots from four leaf discs of each genotype. The asterisk denotes a significant difference determined by single factor ANOVA (P<0.05). (D) Pathogen biomass was measured at the lesion sites by qPCR to determine the ratio of pathogen DNA to cacao DNA 2 d after inoculation. Bars represent four biological replicates, each with three technical replicates. The asterisk denotes a significant difference determined by single factor ANOVA analysis (P<0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4588882&req=5

Figure 2: Functional analysis of TcNPR1. (A) Representative images of lesions from control and TcNPR1 transiently transformed leaves 2 d after Phytophthora tropicalis inoculation. (B) qRT-PCR analysis of TcNPR1 transcript 2 d after vacuum infiltration. Ctrl, control; NPR1, TcNPR1overexpression. Bars represent means ±SE of three biological replicates. (C) Average lesion areas from control and TcNPR1 overexpressing leaves were measured 3 d after inoculation using ImageJ. Bars represent the means ±SE of measurements from 12 lesion spots from four leaf discs of each genotype. The asterisk denotes a significant difference determined by single factor ANOVA (P<0.05). (D) Pathogen biomass was measured at the lesion sites by qPCR to determine the ratio of pathogen DNA to cacao DNA 2 d after inoculation. Bars represent four biological replicates, each with three technical replicates. The asterisk denotes a significant difference determined by single factor ANOVA analysis (P<0.05).
Bottom Line: Varietal differences in defence responses provide insights into the mechanisms of resistance and are a key resource for plant breeders.Transient overexpression of TcNPR1, a major transcriptional regulator of the SA-dependent plant immune system, also increased pathogen tolerance in cacao leaves.These experiments revealed critical insights regarding the molecular differences between cacao varieties, which will allow a better understanding of defence mechanisms to help guide breeding programmes.

View Article: PubMed Central - PubMed

Affiliation: The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

No MeSH data available.


Related in: MedlinePlus