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A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.).

Wang D, Chen X, Zhang Z, Liu D, Song G, Kong X, Geng S, Yang J, Wang B, Wu L, Li A, Mao L - J. Exp. Bot. (2015)

Bottom Line: Moreover, an Arabidopsis BREVIPEDECELLUS/KNAT1 homologue NtBP-Like (NtBPL) was significantly up-regulated in NtSVP-RNAi plants.Disruption of NtBPL decreased pedicel lengths and shortened cortex cells.Microarray analysis showed that down-regulation of NtBPL resulted in differential expression of genes associated with a number of hormone biogenesis and signalling genes such as those for auxin and gibberellin.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

No MeSH data available.


Expression analysis of tobacco homologues of Arabidopsis genes for pedicel development. (A) qRT-PCR detection of transcript levels of differently expressed GA biosynthetic genes (Heinrich et al., 2013) in pedicels of NtBPL-RNAi plants compared with the wild type (WT) according to microarray results. NtACTIN9 was used as an internal control. (B) qRT-PCR detection of transcript levels of homologues to Arabidopsis pedicel-regulating genes in pedicels of NtSVP-RNAi and WT plants. Sequence information was obtained from the SOL Genomics Network by Blast using Arabidopsis genes as queries. NtAS1, mRNA_73569; NtAS2, mRNA_104917; NtER, mRNA_128857; NtCRM1, mRNA_59455; NtYucca5, mRNA_8257). (C) qRT-PCR detection of transcript levels of the above genes in pedicels of NtBPL-RNAi and WT plants.
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Figure 5: Expression analysis of tobacco homologues of Arabidopsis genes for pedicel development. (A) qRT-PCR detection of transcript levels of differently expressed GA biosynthetic genes (Heinrich et al., 2013) in pedicels of NtBPL-RNAi plants compared with the wild type (WT) according to microarray results. NtACTIN9 was used as an internal control. (B) qRT-PCR detection of transcript levels of homologues to Arabidopsis pedicel-regulating genes in pedicels of NtSVP-RNAi and WT plants. Sequence information was obtained from the SOL Genomics Network by Blast using Arabidopsis genes as queries. NtAS1, mRNA_73569; NtAS2, mRNA_104917; NtER, mRNA_128857; NtCRM1, mRNA_59455; NtYucca5, mRNA_8257). (C) qRT-PCR detection of transcript levels of the above genes in pedicels of NtBPL-RNAi and WT plants.

Mentions: Those differentially expressed genes were then further classified using the MapMan program (http://mapman.gabipd.org), and it was found that the hormone metabolism pathway was notably enriched among down-regulated genes (Supplementary Fig. S8C at JXB online). Hormone biogenesis and signalling genes for auxin, ethylene, gibberellin (GA), and brassinosteriod were found among the differentially expressed genes (Supplementary Table S1 at JXB online). There were seven genes associated with GA functions, five of which were for GA biosynthesis, including homologues of ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurenoic acid oxidase (KAO), and GA 3-oxidase (GA3OX1/2) (Yamaguchi, 2008). These genes were subject to further characterization by qRT-PCR. As shown in Fig. 5A, NtCPS, NtKS, and NtKAO1 were down-regulated in NtBPL-RNAi lines, together with two homologues, NtGA3OX1 and NtGA3OX2, whose products putatively catalyse the last step of GA biogenesis. These data indicate that disruption of NtBPL may significantly affect GA biogenesis which may play a significant role in tobacco pedicel development. This is consistent with GA as a key hormone for plant cell elongation and division (Shani et al., 2013).


A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.).

Wang D, Chen X, Zhang Z, Liu D, Song G, Kong X, Geng S, Yang J, Wang B, Wu L, Li A, Mao L - J. Exp. Bot. (2015)

Expression analysis of tobacco homologues of Arabidopsis genes for pedicel development. (A) qRT-PCR detection of transcript levels of differently expressed GA biosynthetic genes (Heinrich et al., 2013) in pedicels of NtBPL-RNAi plants compared with the wild type (WT) according to microarray results. NtACTIN9 was used as an internal control. (B) qRT-PCR detection of transcript levels of homologues to Arabidopsis pedicel-regulating genes in pedicels of NtSVP-RNAi and WT plants. Sequence information was obtained from the SOL Genomics Network by Blast using Arabidopsis genes as queries. NtAS1, mRNA_73569; NtAS2, mRNA_104917; NtER, mRNA_128857; NtCRM1, mRNA_59455; NtYucca5, mRNA_8257). (C) qRT-PCR detection of transcript levels of the above genes in pedicels of NtBPL-RNAi and WT plants.
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Related In: Results  -  Collection

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Figure 5: Expression analysis of tobacco homologues of Arabidopsis genes for pedicel development. (A) qRT-PCR detection of transcript levels of differently expressed GA biosynthetic genes (Heinrich et al., 2013) in pedicels of NtBPL-RNAi plants compared with the wild type (WT) according to microarray results. NtACTIN9 was used as an internal control. (B) qRT-PCR detection of transcript levels of homologues to Arabidopsis pedicel-regulating genes in pedicels of NtSVP-RNAi and WT plants. Sequence information was obtained from the SOL Genomics Network by Blast using Arabidopsis genes as queries. NtAS1, mRNA_73569; NtAS2, mRNA_104917; NtER, mRNA_128857; NtCRM1, mRNA_59455; NtYucca5, mRNA_8257). (C) qRT-PCR detection of transcript levels of the above genes in pedicels of NtBPL-RNAi and WT plants.
Mentions: Those differentially expressed genes were then further classified using the MapMan program (http://mapman.gabipd.org), and it was found that the hormone metabolism pathway was notably enriched among down-regulated genes (Supplementary Fig. S8C at JXB online). Hormone biogenesis and signalling genes for auxin, ethylene, gibberellin (GA), and brassinosteriod were found among the differentially expressed genes (Supplementary Table S1 at JXB online). There were seven genes associated with GA functions, five of which were for GA biosynthesis, including homologues of ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurenoic acid oxidase (KAO), and GA 3-oxidase (GA3OX1/2) (Yamaguchi, 2008). These genes were subject to further characterization by qRT-PCR. As shown in Fig. 5A, NtCPS, NtKS, and NtKAO1 were down-regulated in NtBPL-RNAi lines, together with two homologues, NtGA3OX1 and NtGA3OX2, whose products putatively catalyse the last step of GA biogenesis. These data indicate that disruption of NtBPL may significantly affect GA biogenesis which may play a significant role in tobacco pedicel development. This is consistent with GA as a key hormone for plant cell elongation and division (Shani et al., 2013).

Bottom Line: Moreover, an Arabidopsis BREVIPEDECELLUS/KNAT1 homologue NtBP-Like (NtBPL) was significantly up-regulated in NtSVP-RNAi plants.Disruption of NtBPL decreased pedicel lengths and shortened cortex cells.Microarray analysis showed that down-regulation of NtBPL resulted in differential expression of genes associated with a number of hormone biogenesis and signalling genes such as those for auxin and gibberellin.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

No MeSH data available.