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A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.).

Wang D, Chen X, Zhang Z, Liu D, Song G, Kong X, Geng S, Yang J, Wang B, Wu L, Li A, Mao L - J. Exp. Bot. (2015)

Bottom Line: Moreover, an Arabidopsis BREVIPEDECELLUS/KNAT1 homologue NtBP-Like (NtBPL) was significantly up-regulated in NtSVP-RNAi plants.Disruption of NtBPL decreased pedicel lengths and shortened cortex cells.Microarray analysis showed that down-regulation of NtBPL resulted in differential expression of genes associated with a number of hormone biogenesis and signalling genes such as those for auxin and gibberellin.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

No MeSH data available.


Characterization of NtBPL transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtBPL-OE, and NtBPL-RNAi plants. Scale bar=20mm. (B) The flowers of WT, NtBPL-OE, and NtBPL-RNAi, with pedicels of different lengths as indicated by white boxes. Scale bars=10mm. (C) Detection of the expression levels of NtBPL and two closely related homologues NTH20 and NTH22 in NtBPL transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control. (D) Lengths of pedicels in WT, NtBPL-OE, and NtBPL-RNAi plants at anthesis (n=8×5, 40 pedicels from five individual plants per genotype). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (E) Quantification of pedicel cortex cell lengths of WT and NtBPL transgenic plants at anthesis (n=30×3×5, 15 pedicel photographs were prepared from five individual plants, and 30 cortex cells were measured for each photograph). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t test).
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Figure 2: Characterization of NtBPL transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtBPL-OE, and NtBPL-RNAi plants. Scale bar=20mm. (B) The flowers of WT, NtBPL-OE, and NtBPL-RNAi, with pedicels of different lengths as indicated by white boxes. Scale bars=10mm. (C) Detection of the expression levels of NtBPL and two closely related homologues NTH20 and NTH22 in NtBPL transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control. (D) Lengths of pedicels in WT, NtBPL-OE, and NtBPL-RNAi plants at anthesis (n=8×5, 40 pedicels from five individual plants per genotype). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (E) Quantification of pedicel cortex cell lengths of WT and NtBPL transgenic plants at anthesis (n=30×3×5, 15 pedicel photographs were prepared from five individual plants, and 30 cortex cells were measured for each photograph). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t test).

Mentions: To identify whether NtBPL plays a role in tobacco pedicel development, transgenic plants were generated comprising 18 NtBPL-RNAi lines and 15 NtBPL-OE lines. Four lines for each genotype were further studied; these exhibited clear morphological changes in their inflorescences compared with wild-type plant (Fig. 2A, C). As shown in Fig. 2C, the expression of NtBPL was down-regulated in RNAi lines and increased in OE lines, while two closely related genes NTH20 and NTH22 were not affected. Unlike NtSVP transgenic plants, transgenic manipulation of NtBPL did not affect tobacco plant height (Supplementary Fig. S5A at JXB online), nor did this have a clear effect on pedicel angles (Supplementary Fig. S5B at JXB online). Despite this, knockdown of NtBPL reduced pedicel lengths an average of 1.8mm, or 16% of that of the wild type, while overexpression of NtBPL caused a significant increase in pedicel length, up to 13.8mm, or a 27.8% increase, as measured from 50 flowers per phenotype (Fig. 2B, D). These data indicate that NtBPL is closely correlated with pedicel elongation and indeed required for pedicel development. Accordingly, cortex cells were found to be reduced ~10% in NtBPL-RNAi plants and increased >15% when NtBPL was overexpressed (Fig. 2E; Supplementary Fig. S5C at JXB online). Because the total number of cortex cells remained statistically unchanged, the variation of pedicel lengths can be attributed to the elongation of cortex cells (Supplementary Fig. S5D at JXB online).


A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.).

Wang D, Chen X, Zhang Z, Liu D, Song G, Kong X, Geng S, Yang J, Wang B, Wu L, Li A, Mao L - J. Exp. Bot. (2015)

Characterization of NtBPL transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtBPL-OE, and NtBPL-RNAi plants. Scale bar=20mm. (B) The flowers of WT, NtBPL-OE, and NtBPL-RNAi, with pedicels of different lengths as indicated by white boxes. Scale bars=10mm. (C) Detection of the expression levels of NtBPL and two closely related homologues NTH20 and NTH22 in NtBPL transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control. (D) Lengths of pedicels in WT, NtBPL-OE, and NtBPL-RNAi plants at anthesis (n=8×5, 40 pedicels from five individual plants per genotype). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (E) Quantification of pedicel cortex cell lengths of WT and NtBPL transgenic plants at anthesis (n=30×3×5, 15 pedicel photographs were prepared from five individual plants, and 30 cortex cells were measured for each photograph). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t test).
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Figure 2: Characterization of NtBPL transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtBPL-OE, and NtBPL-RNAi plants. Scale bar=20mm. (B) The flowers of WT, NtBPL-OE, and NtBPL-RNAi, with pedicels of different lengths as indicated by white boxes. Scale bars=10mm. (C) Detection of the expression levels of NtBPL and two closely related homologues NTH20 and NTH22 in NtBPL transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control. (D) Lengths of pedicels in WT, NtBPL-OE, and NtBPL-RNAi plants at anthesis (n=8×5, 40 pedicels from five individual plants per genotype). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (E) Quantification of pedicel cortex cell lengths of WT and NtBPL transgenic plants at anthesis (n=30×3×5, 15 pedicel photographs were prepared from five individual plants, and 30 cortex cells were measured for each photograph). Values are means ±SE. Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t test).
Mentions: To identify whether NtBPL plays a role in tobacco pedicel development, transgenic plants were generated comprising 18 NtBPL-RNAi lines and 15 NtBPL-OE lines. Four lines for each genotype were further studied; these exhibited clear morphological changes in their inflorescences compared with wild-type plant (Fig. 2A, C). As shown in Fig. 2C, the expression of NtBPL was down-regulated in RNAi lines and increased in OE lines, while two closely related genes NTH20 and NTH22 were not affected. Unlike NtSVP transgenic plants, transgenic manipulation of NtBPL did not affect tobacco plant height (Supplementary Fig. S5A at JXB online), nor did this have a clear effect on pedicel angles (Supplementary Fig. S5B at JXB online). Despite this, knockdown of NtBPL reduced pedicel lengths an average of 1.8mm, or 16% of that of the wild type, while overexpression of NtBPL caused a significant increase in pedicel length, up to 13.8mm, or a 27.8% increase, as measured from 50 flowers per phenotype (Fig. 2B, D). These data indicate that NtBPL is closely correlated with pedicel elongation and indeed required for pedicel development. Accordingly, cortex cells were found to be reduced ~10% in NtBPL-RNAi plants and increased >15% when NtBPL was overexpressed (Fig. 2E; Supplementary Fig. S5C at JXB online). Because the total number of cortex cells remained statistically unchanged, the variation of pedicel lengths can be attributed to the elongation of cortex cells (Supplementary Fig. S5D at JXB online).

Bottom Line: Moreover, an Arabidopsis BREVIPEDECELLUS/KNAT1 homologue NtBP-Like (NtBPL) was significantly up-regulated in NtSVP-RNAi plants.Disruption of NtBPL decreased pedicel lengths and shortened cortex cells.Microarray analysis showed that down-regulation of NtBPL resulted in differential expression of genes associated with a number of hormone biogenesis and signalling genes such as those for auxin and gibberellin.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

No MeSH data available.