Limits...
A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.).

Wang D, Chen X, Zhang Z, Liu D, Song G, Kong X, Geng S, Yang J, Wang B, Wu L, Li A, Mao L - J. Exp. Bot. (2015)

Bottom Line: Moreover, an Arabidopsis BREVIPEDECELLUS/KNAT1 homologue NtBP-Like (NtBPL) was significantly up-regulated in NtSVP-RNAi plants.Disruption of NtBPL decreased pedicel lengths and shortened cortex cells.Microarray analysis showed that down-regulation of NtBPL resulted in differential expression of genes associated with a number of hormone biogenesis and signalling genes such as those for auxin and gibberellin.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

No MeSH data available.


Characterization of NtSVP transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtSVP-RNAi, and NtSVP-OE plants. Scale bars=20mm. (B) Representative flowers from WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis. The portion of pedicel used for histological observation is indicated by a white box. Scale bars=10mm. (C) Longitudinal sections of the pedicels at anthesis of WT and NtSVP transgenic plants. Arrows point to cortex cell areas that are used for measurement. Scale bars=100 μm. (D) Detection of NtSVP and NtSTMADS11 expression in NtSVP transgenic lines by qRT-PCR. The tobacco NtACTIN9 gene was used as an internal control. (E) Distribution of the angles between the pedicels and the inflorescences in WT and NtSVP-RNAi plants. In total, 50 pedicels from five plants were measured as a replicate (n=10×5), and three replications were performed. Values are means ±SE. (F) Lengths of pedicels in WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis (n=10×5, 50 pedicels from five individual plants per genotype). Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (G) Quantification of pedicel cortex cell lengths of WT and NtSVP transgenic plants at anthesis (n=30×3×5, 15 pedicel sections from five individual plants per genotype were used and 30 cortex cells were measured for each section). Values are means ±SE. Asterisks indicate significant differences compared with the WT (**P<0.01, Student’s t-test). (H) Cell numbers in the longitudinal cortex file of pedicels in WT and NtSVP transgenic plants at anthesis (n=3×5, 15 sections from five individual plants per genotype were used). Values are means ±SE. (I) Detection of NtBPL expression in NtSVP transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588881&req=5

Figure 1: Characterization of NtSVP transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtSVP-RNAi, and NtSVP-OE plants. Scale bars=20mm. (B) Representative flowers from WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis. The portion of pedicel used for histological observation is indicated by a white box. Scale bars=10mm. (C) Longitudinal sections of the pedicels at anthesis of WT and NtSVP transgenic plants. Arrows point to cortex cell areas that are used for measurement. Scale bars=100 μm. (D) Detection of NtSVP and NtSTMADS11 expression in NtSVP transgenic lines by qRT-PCR. The tobacco NtACTIN9 gene was used as an internal control. (E) Distribution of the angles between the pedicels and the inflorescences in WT and NtSVP-RNAi plants. In total, 50 pedicels from five plants were measured as a replicate (n=10×5), and three replications were performed. Values are means ±SE. (F) Lengths of pedicels in WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis (n=10×5, 50 pedicels from five individual plants per genotype). Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (G) Quantification of pedicel cortex cell lengths of WT and NtSVP transgenic plants at anthesis (n=30×3×5, 15 pedicel sections from five individual plants per genotype were used and 30 cortex cells were measured for each section). Values are means ±SE. Asterisks indicate significant differences compared with the WT (**P<0.01, Student’s t-test). (H) Cell numbers in the longitudinal cortex file of pedicels in WT and NtSVP transgenic plants at anthesis (n=3×5, 15 sections from five individual plants per genotype were used). Values are means ±SE. (I) Detection of NtBPL expression in NtSVP transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control.

Mentions: To characterize the function of NtSVP, RNAi transgenic lines were produced using a construct containing the less conserved C-terminal portion of NtSVP (Supplementary Fig. S2A at JXB online). Meanwhile, overexpression lines were generated using the DNA fragment containing the complete ORF. A total of 17 independent NtSVP RNAi lines (NtSVP-RNAi) and 11 independent NtSVP overexpression lines (NtSVP-OE) were obtained with evident inflorescence alteration when compared with that of the wild type (Fig. 1A–D). Unexpectedly, no obvious defect was observed in the pedicel AZ, suggesting functional divergence of NtSVP when compared with the tomato SVP homologue J (Supplementary Fig. S2B at JXB online).


A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.).

Wang D, Chen X, Zhang Z, Liu D, Song G, Kong X, Geng S, Yang J, Wang B, Wu L, Li A, Mao L - J. Exp. Bot. (2015)

Characterization of NtSVP transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtSVP-RNAi, and NtSVP-OE plants. Scale bars=20mm. (B) Representative flowers from WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis. The portion of pedicel used for histological observation is indicated by a white box. Scale bars=10mm. (C) Longitudinal sections of the pedicels at anthesis of WT and NtSVP transgenic plants. Arrows point to cortex cell areas that are used for measurement. Scale bars=100 μm. (D) Detection of NtSVP and NtSTMADS11 expression in NtSVP transgenic lines by qRT-PCR. The tobacco NtACTIN9 gene was used as an internal control. (E) Distribution of the angles between the pedicels and the inflorescences in WT and NtSVP-RNAi plants. In total, 50 pedicels from five plants were measured as a replicate (n=10×5), and three replications were performed. Values are means ±SE. (F) Lengths of pedicels in WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis (n=10×5, 50 pedicels from five individual plants per genotype). Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (G) Quantification of pedicel cortex cell lengths of WT and NtSVP transgenic plants at anthesis (n=30×3×5, 15 pedicel sections from five individual plants per genotype were used and 30 cortex cells were measured for each section). Values are means ±SE. Asterisks indicate significant differences compared with the WT (**P<0.01, Student’s t-test). (H) Cell numbers in the longitudinal cortex file of pedicels in WT and NtSVP transgenic plants at anthesis (n=3×5, 15 sections from five individual plants per genotype were used). Values are means ±SE. (I) Detection of NtBPL expression in NtSVP transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588881&req=5

Figure 1: Characterization of NtSVP transgenic plants. (A) Inflorescence morphology of wild-type (WT), NtSVP-RNAi, and NtSVP-OE plants. Scale bars=20mm. (B) Representative flowers from WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis. The portion of pedicel used for histological observation is indicated by a white box. Scale bars=10mm. (C) Longitudinal sections of the pedicels at anthesis of WT and NtSVP transgenic plants. Arrows point to cortex cell areas that are used for measurement. Scale bars=100 μm. (D) Detection of NtSVP and NtSTMADS11 expression in NtSVP transgenic lines by qRT-PCR. The tobacco NtACTIN9 gene was used as an internal control. (E) Distribution of the angles between the pedicels and the inflorescences in WT and NtSVP-RNAi plants. In total, 50 pedicels from five plants were measured as a replicate (n=10×5), and three replications were performed. Values are means ±SE. (F) Lengths of pedicels in WT, NtSVP-RNAi, and NtSVP-OE plants at anthesis (n=10×5, 50 pedicels from five individual plants per genotype). Asterisks indicate significant differences compared with the WT (*P<0.05, **P<0.01, Student’s t-test). (G) Quantification of pedicel cortex cell lengths of WT and NtSVP transgenic plants at anthesis (n=30×3×5, 15 pedicel sections from five individual plants per genotype were used and 30 cortex cells were measured for each section). Values are means ±SE. Asterisks indicate significant differences compared with the WT (**P<0.01, Student’s t-test). (H) Cell numbers in the longitudinal cortex file of pedicels in WT and NtSVP transgenic plants at anthesis (n=3×5, 15 sections from five individual plants per genotype were used). Values are means ±SE. (I) Detection of NtBPL expression in NtSVP transgenic lines by qRT-PCR. NtACTIN9 was used as an internal control.
Mentions: To characterize the function of NtSVP, RNAi transgenic lines were produced using a construct containing the less conserved C-terminal portion of NtSVP (Supplementary Fig. S2A at JXB online). Meanwhile, overexpression lines were generated using the DNA fragment containing the complete ORF. A total of 17 independent NtSVP RNAi lines (NtSVP-RNAi) and 11 independent NtSVP overexpression lines (NtSVP-OE) were obtained with evident inflorescence alteration when compared with that of the wild type (Fig. 1A–D). Unexpectedly, no obvious defect was observed in the pedicel AZ, suggesting functional divergence of NtSVP when compared with the tomato SVP homologue J (Supplementary Fig. S2B at JXB online).

Bottom Line: Moreover, an Arabidopsis BREVIPEDECELLUS/KNAT1 homologue NtBP-Like (NtBPL) was significantly up-regulated in NtSVP-RNAi plants.Disruption of NtBPL decreased pedicel lengths and shortened cortex cells.Microarray analysis showed that down-regulation of NtBPL resulted in differential expression of genes associated with a number of hormone biogenesis and signalling genes such as those for auxin and gibberellin.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

No MeSH data available.