Limits...
Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.


Relative expression profiles of BrAOP2 genes in L143 organs at the seedling stage (2 weeks) and reproductive stage (10 weeks) (horizontal axis). The vertical axis indicates gene expression levels relative to BrAOP2.3 (Bra000848) expression in siliques. Error bars represent the standard deviation from three biological repeats. Different lowercase letters indicate significant differences at P<0.05 using ANOVA analysis followed by a Duncan post-hoc test.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588880&req=5

Figure 10: Relative expression profiles of BrAOP2 genes in L143 organs at the seedling stage (2 weeks) and reproductive stage (10 weeks) (horizontal axis). The vertical axis indicates gene expression levels relative to BrAOP2.3 (Bra000848) expression in siliques. Error bars represent the standard deviation from three biological repeats. Different lowercase letters indicate significant differences at P<0.05 using ANOVA analysis followed by a Duncan post-hoc test.


Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Relative expression profiles of BrAOP2 genes in L143 organs at the seedling stage (2 weeks) and reproductive stage (10 weeks) (horizontal axis). The vertical axis indicates gene expression levels relative to BrAOP2.3 (Bra000848) expression in siliques. Error bars represent the standard deviation from three biological repeats. Different lowercase letters indicate significant differences at P<0.05 using ANOVA analysis followed by a Duncan post-hoc test.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588880&req=5

Figure 10: Relative expression profiles of BrAOP2 genes in L143 organs at the seedling stage (2 weeks) and reproductive stage (10 weeks) (horizontal axis). The vertical axis indicates gene expression levels relative to BrAOP2.3 (Bra000848) expression in siliques. Error bars represent the standard deviation from three biological repeats. Different lowercase letters indicate significant differences at P<0.05 using ANOVA analysis followed by a Duncan post-hoc test.
Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.