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Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.


Histochemical GUS staining of ProBrAOP2:GUS transgenic Arabidopsis lines at different developmental stages. (A) One-week-old seedlings, (B) 3-week-old seedlings, (C) 5-week-old rosette leaves, (D) flowers, (E) immature silique, and (F) mature silique. Plants from stages (A) and (B) were grown on MS medium, and plants from stage (C–F) were grown on soil. A GUS histochemical assay was carried out on three independent single-copy transgenic lines of each BrAOP2 paralogue in the T3 generation and showed the same result.
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Figure 9: Histochemical GUS staining of ProBrAOP2:GUS transgenic Arabidopsis lines at different developmental stages. (A) One-week-old seedlings, (B) 3-week-old seedlings, (C) 5-week-old rosette leaves, (D) flowers, (E) immature silique, and (F) mature silique. Plants from stages (A) and (B) were grown on MS medium, and plants from stage (C–F) were grown on soil. A GUS histochemical assay was carried out on three independent single-copy transgenic lines of each BrAOP2 paralogue in the T3 generation and showed the same result.

Mentions: To better understand the expression of BrAOP2 genes, we generated transgenic plants carrying a GUS reporter driven by each BrAOP2 promoter (ProBrAOP2:GUS). Reporter gene expression in transgenic Arabidopsis lines revealed that BrAOP2.1 and BrAOP2.2 paralogues showed similar and stable expression patterns, while ProBrAOP2.3:GUS lines showed distinct expression patterns during the developing stages of Arabidopsis thaliana (Fig. 9).


Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Histochemical GUS staining of ProBrAOP2:GUS transgenic Arabidopsis lines at different developmental stages. (A) One-week-old seedlings, (B) 3-week-old seedlings, (C) 5-week-old rosette leaves, (D) flowers, (E) immature silique, and (F) mature silique. Plants from stages (A) and (B) were grown on MS medium, and plants from stage (C–F) were grown on soil. A GUS histochemical assay was carried out on three independent single-copy transgenic lines of each BrAOP2 paralogue in the T3 generation and showed the same result.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588880&req=5

Figure 9: Histochemical GUS staining of ProBrAOP2:GUS transgenic Arabidopsis lines at different developmental stages. (A) One-week-old seedlings, (B) 3-week-old seedlings, (C) 5-week-old rosette leaves, (D) flowers, (E) immature silique, and (F) mature silique. Plants from stages (A) and (B) were grown on MS medium, and plants from stage (C–F) were grown on soil. A GUS histochemical assay was carried out on three independent single-copy transgenic lines of each BrAOP2 paralogue in the T3 generation and showed the same result.
Mentions: To better understand the expression of BrAOP2 genes, we generated transgenic plants carrying a GUS reporter driven by each BrAOP2 promoter (ProBrAOP2:GUS). Reporter gene expression in transgenic Arabidopsis lines revealed that BrAOP2.1 and BrAOP2.2 paralogues showed similar and stable expression patterns, while ProBrAOP2.3:GUS lines showed distinct expression patterns during the developing stages of Arabidopsis thaliana (Fig. 9).

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.