Limits...
Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.


Related in: MedlinePlus

Evolutionary relationships and motif structures of Brassicaceae AOP proteins. Phylogenetic analysis was performed based on the conserved sequences at the N and C terminal parts of AOP proteins using the neighbour-joining method (bootstrap test with 1000 replicates) (Tamura et al., 2011). The sequenced Brassicaceae species in BRAD included: lineage I species Arabidopsis thaliana, Arabidopsis lyrata, Capsella rubella, Camelina sativa, and L. alabamica; lineage II species T. halophila, T. salsuginea, S. parvula (synonym of T. parvula), S. irio, B. rapa, B. oleracea, and R. sativus; and an early branching sister of the core Brassicaceae, Aethionema arabicum. Black branches indicate AOP1 proteins, coloured branches indicate AOP2 and AOP3 proteins. The tree is drawn to scale; bar, number of substitutions per site.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588880&req=5

Figure 3: Evolutionary relationships and motif structures of Brassicaceae AOP proteins. Phylogenetic analysis was performed based on the conserved sequences at the N and C terminal parts of AOP proteins using the neighbour-joining method (bootstrap test with 1000 replicates) (Tamura et al., 2011). The sequenced Brassicaceae species in BRAD included: lineage I species Arabidopsis thaliana, Arabidopsis lyrata, Capsella rubella, Camelina sativa, and L. alabamica; lineage II species T. halophila, T. salsuginea, S. parvula (synonym of T. parvula), S. irio, B. rapa, B. oleracea, and R. sativus; and an early branching sister of the core Brassicaceae, Aethionema arabicum. Black branches indicate AOP1 proteins, coloured branches indicate AOP2 and AOP3 proteins. The tree is drawn to scale; bar, number of substitutions per site.

Mentions: The Brassicaceae is a medium-sized family that can be split into two major groups: the Aethionema group and the core group (Franzke et al., 2011). Three major lineages (I, II, and III) of the core group have been proposed based on the sequences of the chloroplast NADH dehydrogenase gene ndhF (Beilstein et al., 2006) and supported by subsequent studies (Koch et al., 2007; Beilstein et al., 2008; Couvreur et al., 2010). To investigate further the evolutionary origin of the three BrAOP2 genes, we first identified all AOP genes in the 13 sequenced Brassicaceae species in BRAD (Cheng et al., 2011) according to their genome annotation information and their syntenic relationship with Arabidopsis thaliana AOP genes (http://brassicadb.org/brad/searchSyntenytPCK.php) (Supplementary Table S2, available at JXB online). We next reconstructed a phylogenetic tree of all deduced AOP amino acid sequences using the maximum-likelihood method (Tamura et al., 2011) (Fig. 3).


Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Evolutionary relationships and motif structures of Brassicaceae AOP proteins. Phylogenetic analysis was performed based on the conserved sequences at the N and C terminal parts of AOP proteins using the neighbour-joining method (bootstrap test with 1000 replicates) (Tamura et al., 2011). The sequenced Brassicaceae species in BRAD included: lineage I species Arabidopsis thaliana, Arabidopsis lyrata, Capsella rubella, Camelina sativa, and L. alabamica; lineage II species T. halophila, T. salsuginea, S. parvula (synonym of T. parvula), S. irio, B. rapa, B. oleracea, and R. sativus; and an early branching sister of the core Brassicaceae, Aethionema arabicum. Black branches indicate AOP1 proteins, coloured branches indicate AOP2 and AOP3 proteins. The tree is drawn to scale; bar, number of substitutions per site.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588880&req=5

Figure 3: Evolutionary relationships and motif structures of Brassicaceae AOP proteins. Phylogenetic analysis was performed based on the conserved sequences at the N and C terminal parts of AOP proteins using the neighbour-joining method (bootstrap test with 1000 replicates) (Tamura et al., 2011). The sequenced Brassicaceae species in BRAD included: lineage I species Arabidopsis thaliana, Arabidopsis lyrata, Capsella rubella, Camelina sativa, and L. alabamica; lineage II species T. halophila, T. salsuginea, S. parvula (synonym of T. parvula), S. irio, B. rapa, B. oleracea, and R. sativus; and an early branching sister of the core Brassicaceae, Aethionema arabicum. Black branches indicate AOP1 proteins, coloured branches indicate AOP2 and AOP3 proteins. The tree is drawn to scale; bar, number of substitutions per site.
Mentions: The Brassicaceae is a medium-sized family that can be split into two major groups: the Aethionema group and the core group (Franzke et al., 2011). Three major lineages (I, II, and III) of the core group have been proposed based on the sequences of the chloroplast NADH dehydrogenase gene ndhF (Beilstein et al., 2006) and supported by subsequent studies (Koch et al., 2007; Beilstein et al., 2008; Couvreur et al., 2010). To investigate further the evolutionary origin of the three BrAOP2 genes, we first identified all AOP genes in the 13 sequenced Brassicaceae species in BRAD (Cheng et al., 2011) according to their genome annotation information and their syntenic relationship with Arabidopsis thaliana AOP genes (http://brassicadb.org/brad/searchSyntenytPCK.php) (Supplementary Table S2, available at JXB online). We next reconstructed a phylogenetic tree of all deduced AOP amino acid sequences using the maximum-likelihood method (Tamura et al., 2011) (Fig. 3).

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.


Related in: MedlinePlus