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Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.


Nucleotide polymorphisms in the three BrAOP2 and AtAOP2 sequences. Each BrAOP2 sequence was compared with the AtAOP2 sequence, and the relative frequency of nucleotide substitution between them was tested. The vertical axis reflects the relative frequency of nucleotide substitution between BrAOP2 and AtAOP2 sequences per sliding window. A sliding window of 50bp with a step width of 10bp was used. Alignment gaps were included in scaling the horizontal axis. The three exons of AtAOP2 are depicted under the horizontal axis. (This figure is available in colour at JXB online.)
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Figure 1: Nucleotide polymorphisms in the three BrAOP2 and AtAOP2 sequences. Each BrAOP2 sequence was compared with the AtAOP2 sequence, and the relative frequency of nucleotide substitution between them was tested. The vertical axis reflects the relative frequency of nucleotide substitution between BrAOP2 and AtAOP2 sequences per sliding window. A sliding window of 50bp with a step width of 10bp was used. Alignment gaps were included in scaling the horizontal axis. The three exons of AtAOP2 are depicted under the horizontal axis. (This figure is available in colour at JXB online.)

Mentions: In B. rapa, three AOP2 paralogues produced by genome replication and distributed in different chromosomes have been annotated in the Brassica Database (BRAD, http://brassicadb.org); namely, BrAOP2.1 (Bra03418, A09), BrAOP2.2 (Bra000848, A03), and BrAOP2.3 (Bra018521, A02). We isolated and sequenced the BrAOP2 genes from B. rapa accession Chiifu-401/42 based on their sequences in BRAD (Cheng et al., 2011). The three BrAOP2 genes contain two conserved (exon 1 and exon 3) and one variable (exon 2) exons compared with AtAOP2 (Fig. 1). As a result, the open reading frames varied from 1287 to 1323bp, encoding proteins of 439, 440, and 428 aa, respectively (Table 1). The deduced amino acid sequences from the three BrAOP2 genes were 74–81% identical (Supplementary Table S1, available at JXB online), and shared high similarity (74–96%) with the functional BoAOP2 protein, and lower similarity (55–60%) with AtAOP2 ecotype Cvi (Supplementary Table S1).


Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.

Zhang J, Liu Z, Liang J, Wu J, Cheng F, Wang X - J. Exp. Bot. (2015)

Nucleotide polymorphisms in the three BrAOP2 and AtAOP2 sequences. Each BrAOP2 sequence was compared with the AtAOP2 sequence, and the relative frequency of nucleotide substitution between them was tested. The vertical axis reflects the relative frequency of nucleotide substitution between BrAOP2 and AtAOP2 sequences per sliding window. A sliding window of 50bp with a step width of 10bp was used. Alignment gaps were included in scaling the horizontal axis. The three exons of AtAOP2 are depicted under the horizontal axis. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588880&req=5

Figure 1: Nucleotide polymorphisms in the three BrAOP2 and AtAOP2 sequences. Each BrAOP2 sequence was compared with the AtAOP2 sequence, and the relative frequency of nucleotide substitution between them was tested. The vertical axis reflects the relative frequency of nucleotide substitution between BrAOP2 and AtAOP2 sequences per sliding window. A sliding window of 50bp with a step width of 10bp was used. Alignment gaps were included in scaling the horizontal axis. The three exons of AtAOP2 are depicted under the horizontal axis. (This figure is available in colour at JXB online.)
Mentions: In B. rapa, three AOP2 paralogues produced by genome replication and distributed in different chromosomes have been annotated in the Brassica Database (BRAD, http://brassicadb.org); namely, BrAOP2.1 (Bra03418, A09), BrAOP2.2 (Bra000848, A03), and BrAOP2.3 (Bra018521, A02). We isolated and sequenced the BrAOP2 genes from B. rapa accession Chiifu-401/42 based on their sequences in BRAD (Cheng et al., 2011). The three BrAOP2 genes contain two conserved (exon 1 and exon 3) and one variable (exon 2) exons compared with AtAOP2 (Fig. 1). As a result, the open reading frames varied from 1287 to 1323bp, encoding proteins of 439, 440, and 428 aa, respectively (Table 1). The deduced amino acid sequences from the three BrAOP2 genes were 74–81% identical (Supplementary Table S1, available at JXB online), and shared high similarity (74–96%) with the functional BoAOP2 protein, and lower similarity (55–60%) with AtAOP2 ecotype Cvi (Supplementary Table S1).

Bottom Line: Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1).Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes.Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.

View Article: PubMed Central - PubMed

Affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Nandajie No. 12, Haidian District, Beijing 100081, PR China.

No MeSH data available.