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The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Changes in the activities of SOD (A), CAT (B), APX (C), GPX (D), ASA/DHA (E) and GSH/GSSG (F) in N. benthamiana plants under cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in these BL-treated plants. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (This figure is available in colour at JXB online.)
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Figure 10: Changes in the activities of SOD (A), CAT (B), APX (C), GPX (D), ASA/DHA (E) and GSH/GSSG (F) in N. benthamiana plants under cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in these BL-treated plants. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (This figure is available in colour at JXB online.)

Mentions: In general, the antioxidant defence machinery protects plants against oxidative stress damage. We next analysed changes in the activities of antioxidant enzymes (SOD, CAT, APX, and GPX) and ratios of GSH/GSSG and ASA/DHA after 3 d of stress. As shown in Fig. 10, the three types of stress conditions all resulted in significant increases in antioxidant enzyme activities and GSH/GSSG or ASA/DHA ratio, and these increases were significantly elevated with BL treatment. Interestingly, pre-treatment with SHAM or silencing of NbAOX1 alleviated the BR-increased SOD, APX, and GPX activities, GSH/GSSG, and ASA/DHA ratios (Fig. 10A, C–F), suggesting more ROS accumulation and oxidative damage, while no significant difference was observed in CAT activities (Fig. 10B).


The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Changes in the activities of SOD (A), CAT (B), APX (C), GPX (D), ASA/DHA (E) and GSH/GSSG (F) in N. benthamiana plants under cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in these BL-treated plants. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4588879&req=5

Figure 10: Changes in the activities of SOD (A), CAT (B), APX (C), GPX (D), ASA/DHA (E) and GSH/GSSG (F) in N. benthamiana plants under cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in these BL-treated plants. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (This figure is available in colour at JXB online.)
Mentions: In general, the antioxidant defence machinery protects plants against oxidative stress damage. We next analysed changes in the activities of antioxidant enzymes (SOD, CAT, APX, and GPX) and ratios of GSH/GSSG and ASA/DHA after 3 d of stress. As shown in Fig. 10, the three types of stress conditions all resulted in significant increases in antioxidant enzyme activities and GSH/GSSG or ASA/DHA ratio, and these increases were significantly elevated with BL treatment. Interestingly, pre-treatment with SHAM or silencing of NbAOX1 alleviated the BR-increased SOD, APX, and GPX activities, GSH/GSSG, and ASA/DHA ratios (Fig. 10A, C–F), suggesting more ROS accumulation and oxidative damage, while no significant difference was observed in CAT activities (Fig. 10B).

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.