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The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Related in: MedlinePlus

AOX alleviates oxidative damage in BR-induced stress tolerance. (A) Cell death was detected by staining with 1.25mg ml–1 of trypan blue. Experiments were repeated three times with similar results. (B) Quantitative measurements of RWC. (C) Quantitative measurements of EL. (D) Quantitative measurements of MDA content. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in the BL-treated plants, and these plants were then challenged with cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)
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Figure 9: AOX alleviates oxidative damage in BR-induced stress tolerance. (A) Cell death was detected by staining with 1.25mg ml–1 of trypan blue. Experiments were repeated three times with similar results. (B) Quantitative measurements of RWC. (C) Quantitative measurements of EL. (D) Quantitative measurements of MDA content. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in the BL-treated plants, and these plants were then challenged with cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)

Mentions: Other indices such as cell death, RWC, EL, and MDA content can also indicate the degree of damage in plants caused by environmental stress. Consistent with the ROS accumulation in Fig. 8, the BL-pre-treated N. benthamiana plants showed lower levels of cell death, EL, and MDA content compared with the control plants, although all of them increased under stress conditions (Fig. 9A–C). In addition, the RWC decreased significantly in the control plants after 3 d of stresses, but under the same stress conditions, BL-treated plants maintained higher RWC content than the control (Fig. 9D). However, these protective effects induced by BRs were again blocked in SHAM pre-treated or NbAOX1 silenced plants (Fig. 9).


The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

AOX alleviates oxidative damage in BR-induced stress tolerance. (A) Cell death was detected by staining with 1.25mg ml–1 of trypan blue. Experiments were repeated three times with similar results. (B) Quantitative measurements of RWC. (C) Quantitative measurements of EL. (D) Quantitative measurements of MDA content. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in the BL-treated plants, and these plants were then challenged with cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 9: AOX alleviates oxidative damage in BR-induced stress tolerance. (A) Cell death was detected by staining with 1.25mg ml–1 of trypan blue. Experiments were repeated three times with similar results. (B) Quantitative measurements of RWC. (C) Quantitative measurements of EL. (D) Quantitative measurements of MDA content. The alternative pathway was inhibited by 1mM SHAM pre-treatment or NbAOX1 silencing in the BL-treated plants, and these plants were then challenged with cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress for 3 d. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)
Mentions: Other indices such as cell death, RWC, EL, and MDA content can also indicate the degree of damage in plants caused by environmental stress. Consistent with the ROS accumulation in Fig. 8, the BL-pre-treated N. benthamiana plants showed lower levels of cell death, EL, and MDA content compared with the control plants, although all of them increased under stress conditions (Fig. 9A–C). In addition, the RWC decreased significantly in the control plants after 3 d of stresses, but under the same stress conditions, BL-treated plants maintained higher RWC content than the control (Fig. 9D). However, these protective effects induced by BRs were again blocked in SHAM pre-treated or NbAOX1 silenced plants (Fig. 9).

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Related in: MedlinePlus