Limits...
The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


BRs induce AOX capacity in response to environmental stress. Alternative respiration (Valt) (A) and NbAOX1 expression (B) in N. benthamiana plants after 12h of cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress. N. benthamiana plants were pre-treated with distilled water or 0.1 μM BL for 24h. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588879&req=5

Figure 6: BRs induce AOX capacity in response to environmental stress. Alternative respiration (Valt) (A) and NbAOX1 expression (B) in N. benthamiana plants after 12h of cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress. N. benthamiana plants were pre-treated with distilled water or 0.1 μM BL for 24h. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)

Mentions: Abiotic stress and pathogen elicitors are known to stimulate the activity of the alternative pathway or at least increase AOX transcripts and/or protein levels (Van Aken et al., 2009; Hanqing et al., 2010). We also investigated whether BR treatment would further affect the alternative pathway capacity under cold, PEG, or HL stresses. The results showed that Valt was higher in BL-treated plants than in control plants under all three types of stress for 12h (Fig. 6A). Consistent with the determined alternative respiration rate, the transcript level of NbAOX1 was much higher in the BL-pre-treated plants than in the non-pre-treated control plants after 12h of stress (Fig. 6B). These data demonstrated that BRs enhance the alternative pathway in response to environmental stress.


The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

BRs induce AOX capacity in response to environmental stress. Alternative respiration (Valt) (A) and NbAOX1 expression (B) in N. benthamiana plants after 12h of cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress. N. benthamiana plants were pre-treated with distilled water or 0.1 μM BL for 24h. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588879&req=5

Figure 6: BRs induce AOX capacity in response to environmental stress. Alternative respiration (Valt) (A) and NbAOX1 expression (B) in N. benthamiana plants after 12h of cold (4 °C), 16% PEG 6000, or HL (600 μmol m–2 s–1) stress. N. benthamiana plants were pre-treated with distilled water or 0.1 μM BL for 24h. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight. (This figure is available in colour at JXB online.)
Mentions: Abiotic stress and pathogen elicitors are known to stimulate the activity of the alternative pathway or at least increase AOX transcripts and/or protein levels (Van Aken et al., 2009; Hanqing et al., 2010). We also investigated whether BR treatment would further affect the alternative pathway capacity under cold, PEG, or HL stresses. The results showed that Valt was higher in BL-treated plants than in control plants under all three types of stress for 12h (Fig. 6A). Consistent with the determined alternative respiration rate, the transcript level of NbAOX1 was much higher in the BL-pre-treated plants than in the non-pre-treated control plants after 12h of stress (Fig. 6B). These data demonstrated that BRs enhance the alternative pathway in response to environmental stress.

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.