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The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Related in: MedlinePlus

Characterization of ROS-responsive motifs in the NbAOX1 promoter. (A) Sequence motifs common to the region 1.15kb upstream of NbAOX1. ROS-responsive motifs are indicated as light blue boxes. (B) A schematic representation of each construct is shown to the left along with the length of each upstream fragment in base pairs. The results from measurement of GUS or LUC activity for each construct are shown. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (C) GUS staining of N. benthamiana leaves transiently transformed with each construct as described in (B), with or without 0.1 μM BL treatment for 24h. Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)
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Figure 5: Characterization of ROS-responsive motifs in the NbAOX1 promoter. (A) Sequence motifs common to the region 1.15kb upstream of NbAOX1. ROS-responsive motifs are indicated as light blue boxes. (B) A schematic representation of each construct is shown to the left along with the length of each upstream fragment in base pairs. The results from measurement of GUS or LUC activity for each construct are shown. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (C) GUS staining of N. benthamiana leaves transiently transformed with each construct as described in (B), with or without 0.1 μM BL treatment for 24h. Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)

Mentions: To further evaluate the role of ROS in BR-regulated NbAOX1 transcription, ROS-response motifs in the NbAOX1 promoter were searched with sequences indicated by Ho et al. (2008) and Petrov et al. (2012), and were found to be distributed primarily at the promoter downstream area (Fig. 5A). These ROS-response motifs were further confirmed by analysis of GUS and LUC activities driven by the NbAOX1 promoter after transient transformation of N. benthamiana leaves. NbAOX1 promoter activity sustained a gradual but mild increase with the increase in the promoter length before BL treatment. However, the more ROS-response motifs in the promoter fragments, the sharper increase in promoter activity derived by BL treatment (Fig. 5B). The result was also confirmed by GUS staining (Fig. 5C).


The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Characterization of ROS-responsive motifs in the NbAOX1 promoter. (A) Sequence motifs common to the region 1.15kb upstream of NbAOX1. ROS-responsive motifs are indicated as light blue boxes. (B) A schematic representation of each construct is shown to the left along with the length of each upstream fragment in base pairs. The results from measurement of GUS or LUC activity for each construct are shown. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (C) GUS staining of N. benthamiana leaves transiently transformed with each construct as described in (B), with or without 0.1 μM BL treatment for 24h. Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 5: Characterization of ROS-responsive motifs in the NbAOX1 promoter. (A) Sequence motifs common to the region 1.15kb upstream of NbAOX1. ROS-responsive motifs are indicated as light blue boxes. (B) A schematic representation of each construct is shown to the left along with the length of each upstream fragment in base pairs. The results from measurement of GUS or LUC activity for each construct are shown. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (C) GUS staining of N. benthamiana leaves transiently transformed with each construct as described in (B), with or without 0.1 μM BL treatment for 24h. Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)
Mentions: To further evaluate the role of ROS in BR-regulated NbAOX1 transcription, ROS-response motifs in the NbAOX1 promoter were searched with sequences indicated by Ho et al. (2008) and Petrov et al. (2012), and were found to be distributed primarily at the promoter downstream area (Fig. 5A). These ROS-response motifs were further confirmed by analysis of GUS and LUC activities driven by the NbAOX1 promoter after transient transformation of N. benthamiana leaves. NbAOX1 promoter activity sustained a gradual but mild increase with the increase in the promoter length before BL treatment. However, the more ROS-response motifs in the promoter fragments, the sharper increase in promoter activity derived by BL treatment (Fig. 5B). The result was also confirmed by GUS staining (Fig. 5C).

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Related in: MedlinePlus