Limits...
The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Related in: MedlinePlus

Effects of H2O2 on BR-induced NbAOX1 promoter activity. (A, B) Relative GUS (A) or LUC (B) activity under the control of the NbAOX1 promoter in 5mM DMTU pre-treatment plants as influenced by 0.1 μM BL. (C, D) Relative GUS (C) or LUC (D) activity under the control of the NbAOX1 promoter in NbRBOHB-silenced plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (E) GUS staining of N. benthamiana leaves transiently transformed with NbAOX1 promoter fused to the GUS reporter gene as described in (A) and (C). Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588879&req=5

Figure 4: Effects of H2O2 on BR-induced NbAOX1 promoter activity. (A, B) Relative GUS (A) or LUC (B) activity under the control of the NbAOX1 promoter in 5mM DMTU pre-treatment plants as influenced by 0.1 μM BL. (C, D) Relative GUS (C) or LUC (D) activity under the control of the NbAOX1 promoter in NbRBOHB-silenced plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (E) GUS staining of N. benthamiana leaves transiently transformed with NbAOX1 promoter fused to the GUS reporter gene as described in (A) and (C). Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)

Mentions: As described above, ROS play an important role in BR-induced NbAOX1 expression. In order to investigate the transcriptional regulatory linkage between NbAOX1 gene and H2O2 accumulation induced by BRs, we analysed the NbAOX1 promoter using the GUS and LUC reporter systems after transient transformation in N. benthamiana leaves. After BL treatment, GUS and LUC activities driven by the NbAOX1 promoter were significantly upregulated when compared with water treatment. However, pre-treatment with DMTU substantially reduced the BR-induced NbAOX1 promoter activity, whereas the pre-treatment had little effect in the absence of BL treatment (Fig. 4A, B). We also tested the transcriptional regulatory in NbRBOHB-silenced plants. Our results showed that BL treatment induced the NbAOX1 promoter in TRV:00 plants obviously, whereas the treatment had little effect in TRV:NbRBOHB plants (Fig. 4C, D). GUS staining showed similar results (Fig. 4E).


The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Effects of H2O2 on BR-induced NbAOX1 promoter activity. (A, B) Relative GUS (A) or LUC (B) activity under the control of the NbAOX1 promoter in 5mM DMTU pre-treatment plants as influenced by 0.1 μM BL. (C, D) Relative GUS (C) or LUC (D) activity under the control of the NbAOX1 promoter in NbRBOHB-silenced plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (E) GUS staining of N. benthamiana leaves transiently transformed with NbAOX1 promoter fused to the GUS reporter gene as described in (A) and (C). Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588879&req=5

Figure 4: Effects of H2O2 on BR-induced NbAOX1 promoter activity. (A, B) Relative GUS (A) or LUC (B) activity under the control of the NbAOX1 promoter in 5mM DMTU pre-treatment plants as influenced by 0.1 μM BL. (C, D) Relative GUS (C) or LUC (D) activity under the control of the NbAOX1 promoter in NbRBOHB-silenced plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. (E) GUS staining of N. benthamiana leaves transiently transformed with NbAOX1 promoter fused to the GUS reporter gene as described in (A) and (C). Experiments were repeated three times with similar results. (This figure is available in colour at JXB online.)
Mentions: As described above, ROS play an important role in BR-induced NbAOX1 expression. In order to investigate the transcriptional regulatory linkage between NbAOX1 gene and H2O2 accumulation induced by BRs, we analysed the NbAOX1 promoter using the GUS and LUC reporter systems after transient transformation in N. benthamiana leaves. After BL treatment, GUS and LUC activities driven by the NbAOX1 promoter were significantly upregulated when compared with water treatment. However, pre-treatment with DMTU substantially reduced the BR-induced NbAOX1 promoter activity, whereas the pre-treatment had little effect in the absence of BL treatment (Fig. 4A, B). We also tested the transcriptional regulatory in NbRBOHB-silenced plants. Our results showed that BL treatment induced the NbAOX1 promoter in TRV:00 plants obviously, whereas the treatment had little effect in TRV:NbRBOHB plants (Fig. 4C, D). GUS staining showed similar results (Fig. 4E).

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Related in: MedlinePlus