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The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.


Time course of BR-induced changes in alternative respiration (Valt) (A), NbAOX1 expression (B), H2O2 production (C), and NbRBOHB expression (D). N. benthamiana plants were sprayed with water or 0.1 μM BL, and the sixth leaf of each plant was harvested at the indicated time points for assays of alternative respiration, H2O2 content, and gene expression. Bars represent the mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight.
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Figure 2: Time course of BR-induced changes in alternative respiration (Valt) (A), NbAOX1 expression (B), H2O2 production (C), and NbRBOHB expression (D). N. benthamiana plants were sprayed with water or 0.1 μM BL, and the sixth leaf of each plant was harvested at the indicated time points for assays of alternative respiration, H2O2 content, and gene expression. Bars represent the mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight.

Mentions: In order to investigate the relationship between ROS and alternative pathway in BR signalling, we compared the time course of changes on Valt, H2O2 accumulation, and transcripts levels of NbAOX1, NbRBOHA, and NbRBOHB after BL treatment in N. benthamiana. As shown in Fig. 2, Valt was not significantly altered during the first 6h but substantially increased at 12h and remained elevated up to 36h after BL treatment when compared with water-treated plants (Fig. 2A). Transcripts of NbAOX1 increased at 6h, peaked at 24h, and then exhibited a gradual decline with extended time after BL treatment (Fig. 2B). In contrast, for H2O2 accumulation and transcripts of NbRBOHB, a significant increase occurred as early as 3h, and the maximum levels of H2O2 content and NbRBOHB expression were observed at 24 and 12h after treatment with BL, respectively (Fig. 2C, D). However, the expression of NbRBOHA was not significantly altered after BL treatment in comparison with NbRBOHB (Supplementary Fig. S1, available at JXB online). These results suggested that BRs induce ROS accumulation more rapidly than BRs induce the alternative pathway.


The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.

Deng XG, Zhu T, Zhang DW, Lin HH - J. Exp. Bot. (2015)

Time course of BR-induced changes in alternative respiration (Valt) (A), NbAOX1 expression (B), H2O2 production (C), and NbRBOHB expression (D). N. benthamiana plants were sprayed with water or 0.1 μM BL, and the sixth leaf of each plant was harvested at the indicated time points for assays of alternative respiration, H2O2 content, and gene expression. Bars represent the mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 2: Time course of BR-induced changes in alternative respiration (Valt) (A), NbAOX1 expression (B), H2O2 production (C), and NbRBOHB expression (D). N. benthamiana plants were sprayed with water or 0.1 μM BL, and the sixth leaf of each plant was harvested at the indicated time points for assays of alternative respiration, H2O2 content, and gene expression. Bars represent the mean and standard deviation of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lowercase letters. FW, fresh weight.
Mentions: In order to investigate the relationship between ROS and alternative pathway in BR signalling, we compared the time course of changes on Valt, H2O2 accumulation, and transcripts levels of NbAOX1, NbRBOHA, and NbRBOHB after BL treatment in N. benthamiana. As shown in Fig. 2, Valt was not significantly altered during the first 6h but substantially increased at 12h and remained elevated up to 36h after BL treatment when compared with water-treated plants (Fig. 2A). Transcripts of NbAOX1 increased at 6h, peaked at 24h, and then exhibited a gradual decline with extended time after BL treatment (Fig. 2B). In contrast, for H2O2 accumulation and transcripts of NbRBOHB, a significant increase occurred as early as 3h, and the maximum levels of H2O2 content and NbRBOHB expression were observed at 24 and 12h after treatment with BL, respectively (Fig. 2C, D). However, the expression of NbRBOHA was not significantly altered after BL treatment in comparison with NbRBOHB (Supplementary Fig. S1, available at JXB online). These results suggested that BRs induce ROS accumulation more rapidly than BRs induce the alternative pathway.

Bottom Line: Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis.Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance.Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, PR China.

No MeSH data available.