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FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

Jin S, Jung HS, Chung KS, Lee JH, Ahn JH - J. Exp. Bot. (2015)

Bottom Line: In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions.Previous work has shown that FT is graft-transmissible; by contrast, this study did not detect movement of TSF from the roots into the shoot of the scion plants.Taking these results together, we propose that protein mobility of FT is higher than that of TSF, possibly due to a protein domain that confers mobility and/or protein stability.

View Article: PubMed Central - PubMed

Affiliation: Creative Research Initiatives, Department of Life Sciences, Korea University, Seoul 136-701, South Korea.

No MeSH data available.


FT is more stable than TSF. (A) FT and TSF levels at each time point determined by western blot analysis using anti-T7 antibody (top). Rubisco was used as a loading control (bottom). The numbers below each band denote fold-change relative to the FT or TSF levels at 6h. CHX: cycloheximide; DMSO: dimethyl sulphoxide, the non-CHX control. (B) Relative expression levels of FT and TSF as measured by qPCR. Expression levels of TSF or FT at 6h were set to 1.
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Figure 5: FT is more stable than TSF. (A) FT and TSF levels at each time point determined by western blot analysis using anti-T7 antibody (top). Rubisco was used as a loading control (bottom). The numbers below each band denote fold-change relative to the FT or TSF levels at 6h. CHX: cycloheximide; DMSO: dimethyl sulphoxide, the non-CHX control. (B) Relative expression levels of FT and TSF as measured by qPCR. Expression levels of TSF or FT at 6h were set to 1.

Mentions: Because protein stability is important during the overall process of FT and TSF protein movement, namely, before phloem loading, during transport, and even after delivery to the SAM, the observed difference in flowering induced by FT and TSF may be associated with their different protein stabilities. To test this hypothesis, 7-day-old 35S::TSF:T7 and 35S::FT:T7 seedlings grown on MS media were treated with the protein synthesis inhibitor CHX and FT and TSF levels were analysed at the time points indicated in Fig. 5. Although both FT and TSF levels decreased following CHX treatment, TSF levels decreased more rapidly. TSF levels decreased by approximately 60% after 12h, whereas FT levels decreased by approximately 60% after 18h (Fig. 5A), indicating that FT had a longer half-life. Measurement of TSF:T7 and FT:T7 mRNAs after CHX treatment showed that FT:T7 and TSF:T7 mRNAs were consistently expressed after CHX treatment (Fig. 5B), excluding the possibility that the different decreases in protein levels might result from different decreases in their mRNA levels. These results suggested that the stronger effect of pFT::HA:FT:T7 and 35S::FT:T7 cotyledons in rescuing the flowering time of plants bearing the ft-10 mutation resulted from the higher stability of FT compared with TSF.


FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

Jin S, Jung HS, Chung KS, Lee JH, Ahn JH - J. Exp. Bot. (2015)

FT is more stable than TSF. (A) FT and TSF levels at each time point determined by western blot analysis using anti-T7 antibody (top). Rubisco was used as a loading control (bottom). The numbers below each band denote fold-change relative to the FT or TSF levels at 6h. CHX: cycloheximide; DMSO: dimethyl sulphoxide, the non-CHX control. (B) Relative expression levels of FT and TSF as measured by qPCR. Expression levels of TSF or FT at 6h were set to 1.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 5: FT is more stable than TSF. (A) FT and TSF levels at each time point determined by western blot analysis using anti-T7 antibody (top). Rubisco was used as a loading control (bottom). The numbers below each band denote fold-change relative to the FT or TSF levels at 6h. CHX: cycloheximide; DMSO: dimethyl sulphoxide, the non-CHX control. (B) Relative expression levels of FT and TSF as measured by qPCR. Expression levels of TSF or FT at 6h were set to 1.
Mentions: Because protein stability is important during the overall process of FT and TSF protein movement, namely, before phloem loading, during transport, and even after delivery to the SAM, the observed difference in flowering induced by FT and TSF may be associated with their different protein stabilities. To test this hypothesis, 7-day-old 35S::TSF:T7 and 35S::FT:T7 seedlings grown on MS media were treated with the protein synthesis inhibitor CHX and FT and TSF levels were analysed at the time points indicated in Fig. 5. Although both FT and TSF levels decreased following CHX treatment, TSF levels decreased more rapidly. TSF levels decreased by approximately 60% after 12h, whereas FT levels decreased by approximately 60% after 18h (Fig. 5A), indicating that FT had a longer half-life. Measurement of TSF:T7 and FT:T7 mRNAs after CHX treatment showed that FT:T7 and TSF:T7 mRNAs were consistently expressed after CHX treatment (Fig. 5B), excluding the possibility that the different decreases in protein levels might result from different decreases in their mRNA levels. These results suggested that the stronger effect of pFT::HA:FT:T7 and 35S::FT:T7 cotyledons in rescuing the flowering time of plants bearing the ft-10 mutation resulted from the higher stability of FT compared with TSF.

Bottom Line: In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions.Previous work has shown that FT is graft-transmissible; by contrast, this study did not detect movement of TSF from the roots into the shoot of the scion plants.Taking these results together, we propose that protein mobility of FT is higher than that of TSF, possibly due to a protein domain that confers mobility and/or protein stability.

View Article: PubMed Central - PubMed

Affiliation: Creative Research Initiatives, Department of Life Sciences, Korea University, Seoul 136-701, South Korea.

No MeSH data available.