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FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

Jin S, Jung HS, Chung KS, Lee JH, Ahn JH - J. Exp. Bot. (2015)

Bottom Line: In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions.Previous work has shown that FT is graft-transmissible; by contrast, this study did not detect movement of TSF from the roots into the shoot of the scion plants.Taking these results together, we propose that protein mobility of FT is higher than that of TSF, possibly due to a protein domain that confers mobility and/or protein stability.

View Article: PubMed Central - PubMed

Affiliation: Creative Research Initiatives, Department of Life Sciences, Korea University, Seoul 136-701, South Korea.

No MeSH data available.


Related in: MedlinePlus

Partial rescue of the late-flowering phenotype of ft-10 mutants by TSF misexpression from the FT promoter or by grafting of a 35S::TSF cotyledon. (A) RNA levels of the FT promoter-driven HA:FT:T7 and HA:TSF:T7. Total RNA was extracted from 10-day-old whole seedlings. (B) Distribution of flowering time of pFT::HA:TSF:T7 ft-10, pFT::HA:FT:T7 ft-10, and pTSF::FT ft-10 plants under LD conditions, presented as a heat map. Note that pFT::HA:TSF:T7 shows a weak effect in rescuing the late flowering of ft-10 mutants. n = number of plants measured. (C) Distribution of flowering time of ft-10 recipients grafted to a cotyledon of FT- or TSF-overexpressing lines. Note that flowering in ft-10 recipient mutants grafted to a cotyledon of 35S::TSF:T7 plants was accelerated but its effect was weaker than that of a 35S::FT:T7 cotyledon. n = number of plants measured.
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Figure 4: Partial rescue of the late-flowering phenotype of ft-10 mutants by TSF misexpression from the FT promoter or by grafting of a 35S::TSF cotyledon. (A) RNA levels of the FT promoter-driven HA:FT:T7 and HA:TSF:T7. Total RNA was extracted from 10-day-old whole seedlings. (B) Distribution of flowering time of pFT::HA:TSF:T7 ft-10, pFT::HA:FT:T7 ft-10, and pTSF::FT ft-10 plants under LD conditions, presented as a heat map. Note that pFT::HA:TSF:T7 shows a weak effect in rescuing the late flowering of ft-10 mutants. n = number of plants measured. (C) Distribution of flowering time of ft-10 recipients grafted to a cotyledon of FT- or TSF-overexpressing lines. Note that flowering in ft-10 recipient mutants grafted to a cotyledon of 35S::TSF:T7 plants was accelerated but its effect was weaker than that of a 35S::FT:T7 cotyledon. n = number of plants measured.

Mentions: Because TSF expression in the hypocotyl or rootstock failed to accelerate flowering, we next investigated whether TSF expression under the control of the FT promoter (Adrian et al., 2010) could accelerate flowering. To this end, the flowering time of pFT::HA:FT:T7 ft-10 and pFT::HA:TSF:T7 ft-10 plants was measured. qPCR was used to confirm the expression of the FT promoter-driven FT and TSF in homozygous lines (Fig. 4A). Flowering time measurement revealed that the pFT::HA:FT:T7 ft-10 plants flowered with 11.2 leaves, indicating that pFT::HA:FT:T7 almost completely rescued the late flowering of ft-10 mutants (wild-type plants = 12.1 leaves and ft-10 plants = 37.4 leaves) (Fig. 4B). By contrast, homozygous pFT::HA:TSF:T7 ft-10 plants were slightly later flowering (18.9 leaves; P<0.0005) than pFT::HA:FT:T7 ft-10 plants. We also tested whether the TSF promoter-driven FT affected the flowering time of ft-10 mutants. pTSF::FT ft-10 plants showed acceleration of flowering (13.9 leaves).


FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

Jin S, Jung HS, Chung KS, Lee JH, Ahn JH - J. Exp. Bot. (2015)

Partial rescue of the late-flowering phenotype of ft-10 mutants by TSF misexpression from the FT promoter or by grafting of a 35S::TSF cotyledon. (A) RNA levels of the FT promoter-driven HA:FT:T7 and HA:TSF:T7. Total RNA was extracted from 10-day-old whole seedlings. (B) Distribution of flowering time of pFT::HA:TSF:T7 ft-10, pFT::HA:FT:T7 ft-10, and pTSF::FT ft-10 plants under LD conditions, presented as a heat map. Note that pFT::HA:TSF:T7 shows a weak effect in rescuing the late flowering of ft-10 mutants. n = number of plants measured. (C) Distribution of flowering time of ft-10 recipients grafted to a cotyledon of FT- or TSF-overexpressing lines. Note that flowering in ft-10 recipient mutants grafted to a cotyledon of 35S::TSF:T7 plants was accelerated but its effect was weaker than that of a 35S::FT:T7 cotyledon. n = number of plants measured.
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Related In: Results  -  Collection

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Figure 4: Partial rescue of the late-flowering phenotype of ft-10 mutants by TSF misexpression from the FT promoter or by grafting of a 35S::TSF cotyledon. (A) RNA levels of the FT promoter-driven HA:FT:T7 and HA:TSF:T7. Total RNA was extracted from 10-day-old whole seedlings. (B) Distribution of flowering time of pFT::HA:TSF:T7 ft-10, pFT::HA:FT:T7 ft-10, and pTSF::FT ft-10 plants under LD conditions, presented as a heat map. Note that pFT::HA:TSF:T7 shows a weak effect in rescuing the late flowering of ft-10 mutants. n = number of plants measured. (C) Distribution of flowering time of ft-10 recipients grafted to a cotyledon of FT- or TSF-overexpressing lines. Note that flowering in ft-10 recipient mutants grafted to a cotyledon of 35S::TSF:T7 plants was accelerated but its effect was weaker than that of a 35S::FT:T7 cotyledon. n = number of plants measured.
Mentions: Because TSF expression in the hypocotyl or rootstock failed to accelerate flowering, we next investigated whether TSF expression under the control of the FT promoter (Adrian et al., 2010) could accelerate flowering. To this end, the flowering time of pFT::HA:FT:T7 ft-10 and pFT::HA:TSF:T7 ft-10 plants was measured. qPCR was used to confirm the expression of the FT promoter-driven FT and TSF in homozygous lines (Fig. 4A). Flowering time measurement revealed that the pFT::HA:FT:T7 ft-10 plants flowered with 11.2 leaves, indicating that pFT::HA:FT:T7 almost completely rescued the late flowering of ft-10 mutants (wild-type plants = 12.1 leaves and ft-10 plants = 37.4 leaves) (Fig. 4B). By contrast, homozygous pFT::HA:TSF:T7 ft-10 plants were slightly later flowering (18.9 leaves; P<0.0005) than pFT::HA:FT:T7 ft-10 plants. We also tested whether the TSF promoter-driven FT affected the flowering time of ft-10 mutants. pTSF::FT ft-10 plants showed acceleration of flowering (13.9 leaves).

Bottom Line: In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions.Previous work has shown that FT is graft-transmissible; by contrast, this study did not detect movement of TSF from the roots into the shoot of the scion plants.Taking these results together, we propose that protein mobility of FT is higher than that of TSF, possibly due to a protein domain that confers mobility and/or protein stability.

View Article: PubMed Central - PubMed

Affiliation: Creative Research Initiatives, Department of Life Sciences, Korea University, Seoul 136-701, South Korea.

No MeSH data available.


Related in: MedlinePlus