Limits...
FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

Jin S, Jung HS, Chung KS, Lee JH, Ahn JH - J. Exp. Bot. (2015)

Bottom Line: In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions.Previous work has shown that FT is graft-transmissible; by contrast, this study did not detect movement of TSF from the roots into the shoot of the scion plants.Taking these results together, we propose that protein mobility of FT is higher than that of TSF, possibly due to a protein domain that confers mobility and/or protein stability.

View Article: PubMed Central - PubMed

Affiliation: Creative Research Initiatives, Department of Life Sciences, Korea University, Seoul 136-701, South Korea.

No MeSH data available.


Related in: MedlinePlus

Effect of grafting wild-type (WT) and TSF-expressing rootstocks on the flowering time of scion plants under LD conditions. (A) Flowering time of butt-grafted plants. Five-day-old scion and rootstock plants were grafted. Student’s t-test analysis indicated that neither the difference in flowering time between ft/ft and ft/ft tsf plants nor the difference in flowering time between ft tsf/ft tsf and ft tsf/ft plants was statistically significant. (B) Effect of age of wild-type rootstock plants on flowering time of ft tsf scions. Student’s t-test analysis indicated that the flowering time of ft tsf/WT Col (D7) and ft tsf/WT Col (D17) plants did not significantly differ from that of ft tsf/ft tsf grafted plants. Note that the grafted plants in this experiment flowered earlier than in other experiments due to different growth conditions with stronger light intensity. D: day. (C) Strong acceleration of flowering of the ft tsf scion by overexpression of FT and a weak effect of overexpression of TSF. Scale bar = 1cm. (D) Western blot analysis showing the absence of TSF:T7 protein in the shoot apex of ft tsf scion plants grafted to 35S::TSF:T7 plants. In contrast, a band (arrow) was detected in protein extracts from the shoot apex of ft tsf scion plants grafted to 35S::FT:T7 plants. A 5 µg aliquot of protein extract prepared from 35S::FT:T7 plants was used as a positive control (PC). (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588878&req=5

Figure 1: Effect of grafting wild-type (WT) and TSF-expressing rootstocks on the flowering time of scion plants under LD conditions. (A) Flowering time of butt-grafted plants. Five-day-old scion and rootstock plants were grafted. Student’s t-test analysis indicated that neither the difference in flowering time between ft/ft and ft/ft tsf plants nor the difference in flowering time between ft tsf/ft tsf and ft tsf/ft plants was statistically significant. (B) Effect of age of wild-type rootstock plants on flowering time of ft tsf scions. Student’s t-test analysis indicated that the flowering time of ft tsf/WT Col (D7) and ft tsf/WT Col (D17) plants did not significantly differ from that of ft tsf/ft tsf grafted plants. Note that the grafted plants in this experiment flowered earlier than in other experiments due to different growth conditions with stronger light intensity. D: day. (C) Strong acceleration of flowering of the ft tsf scion by overexpression of FT and a weak effect of overexpression of TSF. Scale bar = 1cm. (D) Western blot analysis showing the absence of TSF:T7 protein in the shoot apex of ft tsf scion plants grafted to 35S::TSF:T7 plants. In contrast, a band (arrow) was detected in protein extracts from the shoot apex of ft tsf scion plants grafted to 35S::FT:T7 plants. A 5 µg aliquot of protein extract prepared from 35S::FT:T7 plants was used as a positive control (PC). (This figure is available in colour at JXB online.)

Mentions: To test whether the high expression of TSF in the hypocotyl during the vegetative phase (Supplementary Fig. S1) regulated flowering, it was first examined whether butt-grafting a wild-type hypocotyl could alter flowering time. Either ft single mutants or ft tsf double mutants were used as the rootstock and ft tsf double mutants were used as the scion. To exclude the possibility of TSF expressed in the hypocotyl of the scion affecting the result, the hypocotyl was removed from the scion (Supplementary Fig. S2). If TSF in the hypocotyl of the rootstock is graft-transmissible and can regulate flowering, the flowering of ft tsf scion plants grafted to a ft rootstock (a grafting combination hereafter described as ft tsf/ft, scion/rootstock) should be accelerated, albeit slightly, as the ft rootstock plants carried an endogenous wild-type TSF gene. However, the ft tsf/ft and ft tsf/ft tsf plants flowered with similar numbers of leaves (69.5 versus 64.0 leaves; P>0.07) (Fig. 1A and Supplementary Fig. S3). Similarly, using ft tsf double mutants as a rootstock did not delay the flowering time of ft scion plants. ft/ft and ft/ft tsf plants flowered with similar numbers of leaves (45.8 versus 43.5 leaves; P>0.19).


FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

Jin S, Jung HS, Chung KS, Lee JH, Ahn JH - J. Exp. Bot. (2015)

Effect of grafting wild-type (WT) and TSF-expressing rootstocks on the flowering time of scion plants under LD conditions. (A) Flowering time of butt-grafted plants. Five-day-old scion and rootstock plants were grafted. Student’s t-test analysis indicated that neither the difference in flowering time between ft/ft and ft/ft tsf plants nor the difference in flowering time between ft tsf/ft tsf and ft tsf/ft plants was statistically significant. (B) Effect of age of wild-type rootstock plants on flowering time of ft tsf scions. Student’s t-test analysis indicated that the flowering time of ft tsf/WT Col (D7) and ft tsf/WT Col (D17) plants did not significantly differ from that of ft tsf/ft tsf grafted plants. Note that the grafted plants in this experiment flowered earlier than in other experiments due to different growth conditions with stronger light intensity. D: day. (C) Strong acceleration of flowering of the ft tsf scion by overexpression of FT and a weak effect of overexpression of TSF. Scale bar = 1cm. (D) Western blot analysis showing the absence of TSF:T7 protein in the shoot apex of ft tsf scion plants grafted to 35S::TSF:T7 plants. In contrast, a band (arrow) was detected in protein extracts from the shoot apex of ft tsf scion plants grafted to 35S::FT:T7 plants. A 5 µg aliquot of protein extract prepared from 35S::FT:T7 plants was used as a positive control (PC). (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588878&req=5

Figure 1: Effect of grafting wild-type (WT) and TSF-expressing rootstocks on the flowering time of scion plants under LD conditions. (A) Flowering time of butt-grafted plants. Five-day-old scion and rootstock plants were grafted. Student’s t-test analysis indicated that neither the difference in flowering time between ft/ft and ft/ft tsf plants nor the difference in flowering time between ft tsf/ft tsf and ft tsf/ft plants was statistically significant. (B) Effect of age of wild-type rootstock plants on flowering time of ft tsf scions. Student’s t-test analysis indicated that the flowering time of ft tsf/WT Col (D7) and ft tsf/WT Col (D17) plants did not significantly differ from that of ft tsf/ft tsf grafted plants. Note that the grafted plants in this experiment flowered earlier than in other experiments due to different growth conditions with stronger light intensity. D: day. (C) Strong acceleration of flowering of the ft tsf scion by overexpression of FT and a weak effect of overexpression of TSF. Scale bar = 1cm. (D) Western blot analysis showing the absence of TSF:T7 protein in the shoot apex of ft tsf scion plants grafted to 35S::TSF:T7 plants. In contrast, a band (arrow) was detected in protein extracts from the shoot apex of ft tsf scion plants grafted to 35S::FT:T7 plants. A 5 µg aliquot of protein extract prepared from 35S::FT:T7 plants was used as a positive control (PC). (This figure is available in colour at JXB online.)
Mentions: To test whether the high expression of TSF in the hypocotyl during the vegetative phase (Supplementary Fig. S1) regulated flowering, it was first examined whether butt-grafting a wild-type hypocotyl could alter flowering time. Either ft single mutants or ft tsf double mutants were used as the rootstock and ft tsf double mutants were used as the scion. To exclude the possibility of TSF expressed in the hypocotyl of the scion affecting the result, the hypocotyl was removed from the scion (Supplementary Fig. S2). If TSF in the hypocotyl of the rootstock is graft-transmissible and can regulate flowering, the flowering of ft tsf scion plants grafted to a ft rootstock (a grafting combination hereafter described as ft tsf/ft, scion/rootstock) should be accelerated, albeit slightly, as the ft rootstock plants carried an endogenous wild-type TSF gene. However, the ft tsf/ft and ft tsf/ft tsf plants flowered with similar numbers of leaves (69.5 versus 64.0 leaves; P>0.07) (Fig. 1A and Supplementary Fig. S3). Similarly, using ft tsf double mutants as a rootstock did not delay the flowering time of ft scion plants. ft/ft and ft/ft tsf plants flowered with similar numbers of leaves (45.8 versus 43.5 leaves; P>0.19).

Bottom Line: In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions.Previous work has shown that FT is graft-transmissible; by contrast, this study did not detect movement of TSF from the roots into the shoot of the scion plants.Taking these results together, we propose that protein mobility of FT is higher than that of TSF, possibly due to a protein domain that confers mobility and/or protein stability.

View Article: PubMed Central - PubMed

Affiliation: Creative Research Initiatives, Department of Life Sciences, Korea University, Seoul 136-701, South Korea.

No MeSH data available.


Related in: MedlinePlus