Limits...
Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus

A protein network for the mechanism of mitochondria-mediated male sterility. Histograms and numbers represent protein identification and abundance listed in Supplementary Fig. S5 and Table S1 at JXB online. Cit, citrate synthase; Aco, aconitase; IDH, isocitrate dehydrogenase; α-KGDH, α-ketoglutarate dehydrogenase complex; S-CoA, succinyl-CoA synthetase; MDH, malate dehydrogenase; Tom, outer membrane translocases; Tim, import inner membrane translocases; mHsp70, matrix heat shock protein 70; Hsp60, heat shock protein 60; E1, ubiquitin-activating enzymes; E2, ubiquitin-conjugating enzymes; E3, ubiquitin-ligating enzymes; Ub, ubiquitin; FH4, tetrahydrofolate; SAM, S-adenosylmethionine; MPP, mitochondrial-processing peptidase; MMP, mature mitochondrial protein; Euns, the early uninucleate stage; Tns, the trinucleate stage.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588876&req=5

Figure 5: A protein network for the mechanism of mitochondria-mediated male sterility. Histograms and numbers represent protein identification and abundance listed in Supplementary Fig. S5 and Table S1 at JXB online. Cit, citrate synthase; Aco, aconitase; IDH, isocitrate dehydrogenase; α-KGDH, α-ketoglutarate dehydrogenase complex; S-CoA, succinyl-CoA synthetase; MDH, malate dehydrogenase; Tom, outer membrane translocases; Tim, import inner membrane translocases; mHsp70, matrix heat shock protein 70; Hsp60, heat shock protein 60; E1, ubiquitin-activating enzymes; E2, ubiquitin-conjugating enzymes; E3, ubiquitin-ligating enzymes; Ub, ubiquitin; FH4, tetrahydrofolate; SAM, S-adenosylmethionine; MPP, mitochondrial-processing peptidase; MMP, mature mitochondrial protein; Euns, the early uninucleate stage; Tns, the trinucleate stage.

Mentions: Plant male sterility plays an important role in developmental and molecular studies and hybrid breeding. In the present study, based on the putative functions and changes in the levels of the 68 identified proteins (Supplementary Fig. S5, S7B; Table S1 at JXB online), together with previously published results (Chen et al., 2009, 2010; Zhang et al., 2011, 2013; Ba et al., 2014a, b; Liu et al., 2014), an intriguing mitochondria-mediated male sterility protein network was proposed for wheat (Fig. 5). This network consists of several functional components, including inhibited mtETC and TCA cycle, accelerated biosynthesis and reduced degradation of proteins, generated ROS metabolism, disrupted cell division cycle, and epigenetics.


Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

A protein network for the mechanism of mitochondria-mediated male sterility. Histograms and numbers represent protein identification and abundance listed in Supplementary Fig. S5 and Table S1 at JXB online. Cit, citrate synthase; Aco, aconitase; IDH, isocitrate dehydrogenase; α-KGDH, α-ketoglutarate dehydrogenase complex; S-CoA, succinyl-CoA synthetase; MDH, malate dehydrogenase; Tom, outer membrane translocases; Tim, import inner membrane translocases; mHsp70, matrix heat shock protein 70; Hsp60, heat shock protein 60; E1, ubiquitin-activating enzymes; E2, ubiquitin-conjugating enzymes; E3, ubiquitin-ligating enzymes; Ub, ubiquitin; FH4, tetrahydrofolate; SAM, S-adenosylmethionine; MPP, mitochondrial-processing peptidase; MMP, mature mitochondrial protein; Euns, the early uninucleate stage; Tns, the trinucleate stage.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588876&req=5

Figure 5: A protein network for the mechanism of mitochondria-mediated male sterility. Histograms and numbers represent protein identification and abundance listed in Supplementary Fig. S5 and Table S1 at JXB online. Cit, citrate synthase; Aco, aconitase; IDH, isocitrate dehydrogenase; α-KGDH, α-ketoglutarate dehydrogenase complex; S-CoA, succinyl-CoA synthetase; MDH, malate dehydrogenase; Tom, outer membrane translocases; Tim, import inner membrane translocases; mHsp70, matrix heat shock protein 70; Hsp60, heat shock protein 60; E1, ubiquitin-activating enzymes; E2, ubiquitin-conjugating enzymes; E3, ubiquitin-ligating enzymes; Ub, ubiquitin; FH4, tetrahydrofolate; SAM, S-adenosylmethionine; MPP, mitochondrial-processing peptidase; MMP, mature mitochondrial protein; Euns, the early uninucleate stage; Tns, the trinucleate stage.
Mentions: Plant male sterility plays an important role in developmental and molecular studies and hybrid breeding. In the present study, based on the putative functions and changes in the levels of the 68 identified proteins (Supplementary Fig. S5, S7B; Table S1 at JXB online), together with previously published results (Chen et al., 2009, 2010; Zhang et al., 2011, 2013; Ba et al., 2014a, b; Liu et al., 2014), an intriguing mitochondria-mediated male sterility protein network was proposed for wheat (Fig. 5). This network consists of several functional components, including inhibited mtETC and TCA cycle, accelerated biosynthesis and reduced degradation of proteins, generated ROS metabolism, disrupted cell division cycle, and epigenetics.

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus