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Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus

Functional classification (A) and hierarchical clustering (B) of the identified proteins from florets of PHYMS-XN1376, CMS-XN1376, and MF-XN1376 at the early uninucleate and trinucleate stages. The hierarchical cluster analysis was conducted using the Genesis 1.7.6 procedure (Graz University of Technology, Austria, http://genome.tugraz.at/genesisclient/genesisclient_download.shtml) and the log2-transformed values of abundance change presented in Supplementary Fig. S5 and Table S5 at JXB online. Euns, the early uninucleate stage; Tns, the trinucleate stage; PHYMS, PHYMS-XN1376; CMS, CMS-XN1376; MF, MF-XN1376.
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Figure 3: Functional classification (A) and hierarchical clustering (B) of the identified proteins from florets of PHYMS-XN1376, CMS-XN1376, and MF-XN1376 at the early uninucleate and trinucleate stages. The hierarchical cluster analysis was conducted using the Genesis 1.7.6 procedure (Graz University of Technology, Austria, http://genome.tugraz.at/genesisclient/genesisclient_download.shtml) and the log2-transformed values of abundance change presented in Supplementary Fig. S5 and Table S5 at JXB online. Euns, the early uninucleate stage; Tns, the trinucleate stage; PHYMS, PHYMS-XN1376; CMS, CMS-XN1376; MF, MF-XN1376.

Mentions: Furthermore, based on the biological process of each gene product according to annotations in these databases coupled with the metabolic and functional features of pollen, all of these identities were classified into 12 functional groups (Fig. 3A; Supplementary Table S1 at JXB online), which covered a wide range of pathways and functions, including mitochondrial electron transport (mtETC) and ATP synthesis, the tricarboxylic acid (TCA) cycle, molecular chaperones, protein biosynthesis and degradation, stress and defence, signal transduction, RNA processing, transcription factors, amino acid metabolism, one-carbon metabolism, and nucleotide metabolism. An impressive 62% of these identified proteins were implicated in the first four functional groups, whereas the largest functional group was proteins related to mtETC/ATP synthesis (31%). The other three predominant categories were proteins related to the TCA cycle (10%), molecular chaperones (11%), and protein biosynthesis and degradation (10%). Details of the function of the identified proteins and their abundance profiles in PHYMS-XN1376 and CMS-XN1376 (Fig. 3B) are discussed later.


Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Functional classification (A) and hierarchical clustering (B) of the identified proteins from florets of PHYMS-XN1376, CMS-XN1376, and MF-XN1376 at the early uninucleate and trinucleate stages. The hierarchical cluster analysis was conducted using the Genesis 1.7.6 procedure (Graz University of Technology, Austria, http://genome.tugraz.at/genesisclient/genesisclient_download.shtml) and the log2-transformed values of abundance change presented in Supplementary Fig. S5 and Table S5 at JXB online. Euns, the early uninucleate stage; Tns, the trinucleate stage; PHYMS, PHYMS-XN1376; CMS, CMS-XN1376; MF, MF-XN1376.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 3: Functional classification (A) and hierarchical clustering (B) of the identified proteins from florets of PHYMS-XN1376, CMS-XN1376, and MF-XN1376 at the early uninucleate and trinucleate stages. The hierarchical cluster analysis was conducted using the Genesis 1.7.6 procedure (Graz University of Technology, Austria, http://genome.tugraz.at/genesisclient/genesisclient_download.shtml) and the log2-transformed values of abundance change presented in Supplementary Fig. S5 and Table S5 at JXB online. Euns, the early uninucleate stage; Tns, the trinucleate stage; PHYMS, PHYMS-XN1376; CMS, CMS-XN1376; MF, MF-XN1376.
Mentions: Furthermore, based on the biological process of each gene product according to annotations in these databases coupled with the metabolic and functional features of pollen, all of these identities were classified into 12 functional groups (Fig. 3A; Supplementary Table S1 at JXB online), which covered a wide range of pathways and functions, including mitochondrial electron transport (mtETC) and ATP synthesis, the tricarboxylic acid (TCA) cycle, molecular chaperones, protein biosynthesis and degradation, stress and defence, signal transduction, RNA processing, transcription factors, amino acid metabolism, one-carbon metabolism, and nucleotide metabolism. An impressive 62% of these identified proteins were implicated in the first four functional groups, whereas the largest functional group was proteins related to mtETC/ATP synthesis (31%). The other three predominant categories were proteins related to the TCA cycle (10%), molecular chaperones (11%), and protein biosynthesis and degradation (10%). Details of the function of the identified proteins and their abundance profiles in PHYMS-XN1376 and CMS-XN1376 (Fig. 3B) are discussed later.

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus