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Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus

Venn diagram and scatter plot analysis of DEP spots in PHYMS-XN1376, CMS-XN1376, and MF-XN1376. The numbers of DEP spots which are up- or down-regulated are shown in the different segments; more details are presented in Supplementary Table S5 at JXB online. (A) All of the DEP spots; (B) the up-regulated protein spots; (C) the down-regulated protein spots. E/T-P, the early uninucleate stage/trinucleate stage of PHYMS-1376; E/T-C, the early uninucleate stage/trinucleate stage of CMS-XN1376. (D) The PHYMS-XN1376/CMS-XN1376 ratio of each DEP at the early uninucleate and trinucleate stages from PHYMS-XN1376 is plotted on the x-axis and correspondingly that from CMS-XN1376 is plotted on the y-axis.
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Figure 2: Venn diagram and scatter plot analysis of DEP spots in PHYMS-XN1376, CMS-XN1376, and MF-XN1376. The numbers of DEP spots which are up- or down-regulated are shown in the different segments; more details are presented in Supplementary Table S5 at JXB online. (A) All of the DEP spots; (B) the up-regulated protein spots; (C) the down-regulated protein spots. E/T-P, the early uninucleate stage/trinucleate stage of PHYMS-1376; E/T-C, the early uninucleate stage/trinucleate stage of CMS-XN1376. (D) The PHYMS-XN1376/CMS-XN1376 ratio of each DEP at the early uninucleate and trinucleate stages from PHYMS-XN1376 is plotted on the x-axis and correspondingly that from CMS-XN1376 is plotted on the y-axis.

Mentions: To determine the proteins whose abundance changed in male-sterile mutants, a threshold limit of 1.3-fold (P≤0.05) was set in this study as previously reported (Shen et al., 2009). Statistical analysis of 2-DE gels revealed that 71 protein spots (numbered from 1 to 71) were differentially expressed among MF-XN1376, PHYMS-XN1376, and CMS-XN1376 plants in both the early uninucleate stage and the trinucleate stage (Supplementary Figs S4, S5; Tables S4, S5 at JXB online). Of these, at the early uninucleate stage, there were 35 DEPs in PHYMS-XN1376 (three up- and 32 down-regulated) and 47 DEPs in CMS-XN1376 (21 up- and 26 down-regulated) (Fig. 2A–C; Supplementary Table S5). At the trinucleate stage, there were 66 DEPs in PHYMS-XN1376 (41 up- and 25 down-regulated) and 60 DEPs in CMS-XN1376 (32 up- and 28 down-regulated) (Fig. 2A–C; Supplementary Table S5). Additionally, overlapping DEPs were detected in all of the male-sterile plants, from 30 at the early uninucleate stage up to 58 at the trinucleate stage (Fig. 2A; Supplementary Table S5), and had a corresponding Pearson correlation coefficient from 0.33 up to 0.73 with a P-value <0.01 (Fig. 2D). Therefore, these observations document the changes in abundance of these DEPs along with the occurrence of pollen abortion. These changes show that there are different ways to induce male sterility under mitochondrial protein regulation in the initial stage of anther abortion, and ultimately this regulation makes these ways converge.


Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Venn diagram and scatter plot analysis of DEP spots in PHYMS-XN1376, CMS-XN1376, and MF-XN1376. The numbers of DEP spots which are up- or down-regulated are shown in the different segments; more details are presented in Supplementary Table S5 at JXB online. (A) All of the DEP spots; (B) the up-regulated protein spots; (C) the down-regulated protein spots. E/T-P, the early uninucleate stage/trinucleate stage of PHYMS-1376; E/T-C, the early uninucleate stage/trinucleate stage of CMS-XN1376. (D) The PHYMS-XN1376/CMS-XN1376 ratio of each DEP at the early uninucleate and trinucleate stages from PHYMS-XN1376 is plotted on the x-axis and correspondingly that from CMS-XN1376 is plotted on the y-axis.
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Related In: Results  -  Collection

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Show All Figures
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Figure 2: Venn diagram and scatter plot analysis of DEP spots in PHYMS-XN1376, CMS-XN1376, and MF-XN1376. The numbers of DEP spots which are up- or down-regulated are shown in the different segments; more details are presented in Supplementary Table S5 at JXB online. (A) All of the DEP spots; (B) the up-regulated protein spots; (C) the down-regulated protein spots. E/T-P, the early uninucleate stage/trinucleate stage of PHYMS-1376; E/T-C, the early uninucleate stage/trinucleate stage of CMS-XN1376. (D) The PHYMS-XN1376/CMS-XN1376 ratio of each DEP at the early uninucleate and trinucleate stages from PHYMS-XN1376 is plotted on the x-axis and correspondingly that from CMS-XN1376 is plotted on the y-axis.
Mentions: To determine the proteins whose abundance changed in male-sterile mutants, a threshold limit of 1.3-fold (P≤0.05) was set in this study as previously reported (Shen et al., 2009). Statistical analysis of 2-DE gels revealed that 71 protein spots (numbered from 1 to 71) were differentially expressed among MF-XN1376, PHYMS-XN1376, and CMS-XN1376 plants in both the early uninucleate stage and the trinucleate stage (Supplementary Figs S4, S5; Tables S4, S5 at JXB online). Of these, at the early uninucleate stage, there were 35 DEPs in PHYMS-XN1376 (three up- and 32 down-regulated) and 47 DEPs in CMS-XN1376 (21 up- and 26 down-regulated) (Fig. 2A–C; Supplementary Table S5). At the trinucleate stage, there were 66 DEPs in PHYMS-XN1376 (41 up- and 25 down-regulated) and 60 DEPs in CMS-XN1376 (32 up- and 28 down-regulated) (Fig. 2A–C; Supplementary Table S5). Additionally, overlapping DEPs were detected in all of the male-sterile plants, from 30 at the early uninucleate stage up to 58 at the trinucleate stage (Fig. 2A; Supplementary Table S5), and had a corresponding Pearson correlation coefficient from 0.33 up to 0.73 with a P-value <0.01 (Fig. 2D). Therefore, these observations document the changes in abundance of these DEPs along with the occurrence of pollen abortion. These changes show that there are different ways to induce male sterility under mitochondrial protein regulation in the initial stage of anther abortion, and ultimately this regulation makes these ways converge.

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus