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Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus

Comparison of morphological features of PHYMS-XN1376, CMS-XN1376, and their maintainer line MF-XN1376. Comparison of spikes (A, D, G, J, N, R), and anthers and pistils (B, E, H, K, O, S) of CMS-XN1376, PHYMS-XN1376, and MF-XN1376 plants. The 1% acetocarmine (C, F, I, L, P, T) and 2% I2–KI (M, Q, U) staining of pollen grains. Euns, the early uninucleate stage; Tns, the trinucleate stage. Scale bars=1mm (B, E, H, K, O, S), 100 μm (C, F, I, L, M, P, Q, T, U).
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Figure 1: Comparison of morphological features of PHYMS-XN1376, CMS-XN1376, and their maintainer line MF-XN1376. Comparison of spikes (A, D, G, J, N, R), and anthers and pistils (B, E, H, K, O, S) of CMS-XN1376, PHYMS-XN1376, and MF-XN1376 plants. The 1% acetocarmine (C, F, I, L, P, T) and 2% I2–KI (M, Q, U) staining of pollen grains. Euns, the early uninucleate stage; Tns, the trinucleate stage. Scale bars=1mm (B, E, H, K, O, S), 100 μm (C, F, I, L, M, P, Q, T, U).

Mentions: The morphological features of MF-XN1376, PHYMS- XN1376, and CMS-XN1376 plants were compared. All of these materials have the same nuclear background (T. aestivum nucleus). Compared with MF-XN1376, PHYMS-XN1376 and CMS-XN1376 showed normal spike and floral development but failed to produce any viable pollen at different stages of pollen development (Fig. 1A, D, G, J, N, R); however, the pistils of PHYMS-XN1376 and CMS-XN1376 were normal and able to produce normal seeds when they were backcrossed with fertile pollen (Fig. 1B, E, H, K, O, S). At the early uninucleate stage, there were no visible differences in pollen among the three samples after staining with 1% acetocarmine (Fig. 1C, F, I); however, the anthers of CMS-XN1376 were detectably different from the others (Fig. 1B, E, H), and there were no visible differences between MF-XN1376 and PHYMS-XN1376 (Fig. 1B, E). At the trinucleate stage, compared with MF-XN1376 anthers, the sterile anthers from both PHYMS-XN1376 and CMS-XN1376 plants were small and without mature pollen grains (Fig. 1K, O, S; Supplementary Fig. S2 at JXB online). Futhermore, fertile pollen grains of MF-XN1376 could be clearly distinguished from the sterile pollen of PHYMS-XN1376 and CMS-XN1376 by staining with 1% acetocarmine (Fig. 1L, P, T) and 2% I2–KI (Fig. 1M, Q, U). These results indicated that PHYMS-XN1376 and CMS-XN1376 plants were 100% pollen sterile.


Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivum L.).

Wang S, Zhang G, Zhang Y, Song Q, Chen Z, Wang J, Guo J, Niu N, Wang J, Ma S - J. Exp. Bot. (2015)

Comparison of morphological features of PHYMS-XN1376, CMS-XN1376, and their maintainer line MF-XN1376. Comparison of spikes (A, D, G, J, N, R), and anthers and pistils (B, E, H, K, O, S) of CMS-XN1376, PHYMS-XN1376, and MF-XN1376 plants. The 1% acetocarmine (C, F, I, L, P, T) and 2% I2–KI (M, Q, U) staining of pollen grains. Euns, the early uninucleate stage; Tns, the trinucleate stage. Scale bars=1mm (B, E, H, K, O, S), 100 μm (C, F, I, L, M, P, Q, T, U).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 1: Comparison of morphological features of PHYMS-XN1376, CMS-XN1376, and their maintainer line MF-XN1376. Comparison of spikes (A, D, G, J, N, R), and anthers and pistils (B, E, H, K, O, S) of CMS-XN1376, PHYMS-XN1376, and MF-XN1376 plants. The 1% acetocarmine (C, F, I, L, P, T) and 2% I2–KI (M, Q, U) staining of pollen grains. Euns, the early uninucleate stage; Tns, the trinucleate stage. Scale bars=1mm (B, E, H, K, O, S), 100 μm (C, F, I, L, M, P, Q, T, U).
Mentions: The morphological features of MF-XN1376, PHYMS- XN1376, and CMS-XN1376 plants were compared. All of these materials have the same nuclear background (T. aestivum nucleus). Compared with MF-XN1376, PHYMS-XN1376 and CMS-XN1376 showed normal spike and floral development but failed to produce any viable pollen at different stages of pollen development (Fig. 1A, D, G, J, N, R); however, the pistils of PHYMS-XN1376 and CMS-XN1376 were normal and able to produce normal seeds when they were backcrossed with fertile pollen (Fig. 1B, E, H, K, O, S). At the early uninucleate stage, there were no visible differences in pollen among the three samples after staining with 1% acetocarmine (Fig. 1C, F, I); however, the anthers of CMS-XN1376 were detectably different from the others (Fig. 1B, E, H), and there were no visible differences between MF-XN1376 and PHYMS-XN1376 (Fig. 1B, E). At the trinucleate stage, compared with MF-XN1376 anthers, the sterile anthers from both PHYMS-XN1376 and CMS-XN1376 plants were small and without mature pollen grains (Fig. 1K, O, S; Supplementary Fig. S2 at JXB online). Futhermore, fertile pollen grains of MF-XN1376 could be clearly distinguished from the sterile pollen of PHYMS-XN1376 and CMS-XN1376 by staining with 1% acetocarmine (Fig. 1L, P, T) and 2% I2–KI (Fig. 1M, Q, U). These results indicated that PHYMS-XN1376 and CMS-XN1376 plants were 100% pollen sterile.

Bottom Line: A total of 71 differentially expressed mitochondrial proteins were found to be involved in pollen abortion and further identified by MALDI-TOF/TOF MS (matrix-assisted laser desorption/ionization-time of fight/time of flight mass spectrometry).Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways involved in anther abortion and pollen defects.The results provide intriguing insights into the metabolic pathway of anther abortion induced by CHA-SQ-1 and also give useful clues to identify the crucial proteins of PHYMS and CMS in wheat.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, Shaanxi 712100, P. R. China.

No MeSH data available.


Related in: MedlinePlus