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Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Phenotypes of (A) ZmAAP4-OE and (B) ZmVAAT3-OE Arabidopsis seedlings 12 d after transfer onto ATS medium supplemented with different amino acids. The NO3- treatment served as a control of N source. OE1, OE2 and OE3 represent three independent gene overexpression lines.
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Figure 10: Phenotypes of (A) ZmAAP4-OE and (B) ZmVAAT3-OE Arabidopsis seedlings 12 d after transfer onto ATS medium supplemented with different amino acids. The NO3- treatment served as a control of N source. OE1, OE2 and OE3 represent three independent gene overexpression lines.

Mentions: To further characterize their functions in a plant system, we generated transgenic Arabidopsis seedlings overexpressing ZmAAP4 (ZmAAP4-OE) or ZmVAAT3 (ZmVAAT3-OE), which were grown on a series of ATS media (Schofield et al., 2009) containing different basic amino acids for 12 d for morphological examination. These seedlings showed dramatic alterations in primary root length in many media supplemented by individual amino acids (Fig. 10; Supplementary Tables S10, S11). Compared to WT plants, ZmAAP4-OE seedlings showed reduced primary root growth on media with 17 amino acids except Ala, Ser, Tyr, Asp and Asn (Fig. 10A; Supplementary Tables S9, S11); however, ZmVAAT3-OE seedlings had enhanced primary root length on media supplemented with Leu, Ala, Val, Phe, Met, Arg, Asn or Glu and reduced growth on media with Ser, Cys, Thr, Trp, Lys, Gln, Cit, GABA or His (Fig. 10B; Supplementary Tables S10, S11). Either stimulatory or inhibitory effects of amino-N on primary root growth in ZmAAP4-OE and ZmVAAT3-OE lines suggested substantial uptake of corresponding amino acids mediated by ZmAAP4 and ZmVAAT3 in vivo. In addition to alterations in primary root growth, the number of rosette leaves in ZmAAP4-OE and ZmVAAT3-OE seedlings also varied compared to WT seedlings: ZmAAP4-OE seedlings had more rosette leaves on media with Met, Tyr, Asp, Glu or Asn and less leaves on media containing 17 out of 22 tested amino acids except Met, Tyr, Asp, Glu and Asn (Supplementary Tables S9, S11); ZmVAAT3-OE seedlings had more rosette leaves on media containing Leu, Phe, Tyr or Gln, and less leaves on media with 16 amino acids except Leu, Phe, Tyr, Asp, Gln and GABA (Supplementary Tables S10, S11). The rosette diameter of ZmAAP4-OE seedlings significantly increased on the medium with Ala and decreased on media with 14 amino acids except Ala, Leu, Thr, His, Asp, Asn, Gln and Cit (Supplementary Tables S9, S11); the rosette diameter of ZmVAAT3-OE seedlings significantly increased on media supplemented with Ala and decreased on media containing Gly, Val, Ile, Ser, Tyr, Trp, His, Lys, Glu, Gln or Cit (Supplementary Tables S10, S11). Not surprisingly, the fresh weight of ZmAAP4-OE seedlings increased on the medium supplemented with Ala or Leu and decreased on media with 17 amino acids except Ala, Leu, Asp, Asn or Gln (Supplementary Tables S9, S11); while the fresh weight of ZmVAAT3-OE seedlings significantly increased on media with Met, Pro, Phe or Asn and decreased on media containing Ile, Ser, Cys, Trp, His, Lys, Arg, Glu, Gln or GABA (Supplementary Tables S10, S11).


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Phenotypes of (A) ZmAAP4-OE and (B) ZmVAAT3-OE Arabidopsis seedlings 12 d after transfer onto ATS medium supplemented with different amino acids. The NO3- treatment served as a control of N source. OE1, OE2 and OE3 represent three independent gene overexpression lines.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 10: Phenotypes of (A) ZmAAP4-OE and (B) ZmVAAT3-OE Arabidopsis seedlings 12 d after transfer onto ATS medium supplemented with different amino acids. The NO3- treatment served as a control of N source. OE1, OE2 and OE3 represent three independent gene overexpression lines.
Mentions: To further characterize their functions in a plant system, we generated transgenic Arabidopsis seedlings overexpressing ZmAAP4 (ZmAAP4-OE) or ZmVAAT3 (ZmVAAT3-OE), which were grown on a series of ATS media (Schofield et al., 2009) containing different basic amino acids for 12 d for morphological examination. These seedlings showed dramatic alterations in primary root length in many media supplemented by individual amino acids (Fig. 10; Supplementary Tables S10, S11). Compared to WT plants, ZmAAP4-OE seedlings showed reduced primary root growth on media with 17 amino acids except Ala, Ser, Tyr, Asp and Asn (Fig. 10A; Supplementary Tables S9, S11); however, ZmVAAT3-OE seedlings had enhanced primary root length on media supplemented with Leu, Ala, Val, Phe, Met, Arg, Asn or Glu and reduced growth on media with Ser, Cys, Thr, Trp, Lys, Gln, Cit, GABA or His (Fig. 10B; Supplementary Tables S10, S11). Either stimulatory or inhibitory effects of amino-N on primary root growth in ZmAAP4-OE and ZmVAAT3-OE lines suggested substantial uptake of corresponding amino acids mediated by ZmAAP4 and ZmVAAT3 in vivo. In addition to alterations in primary root growth, the number of rosette leaves in ZmAAP4-OE and ZmVAAT3-OE seedlings also varied compared to WT seedlings: ZmAAP4-OE seedlings had more rosette leaves on media with Met, Tyr, Asp, Glu or Asn and less leaves on media containing 17 out of 22 tested amino acids except Met, Tyr, Asp, Glu and Asn (Supplementary Tables S9, S11); ZmVAAT3-OE seedlings had more rosette leaves on media containing Leu, Phe, Tyr or Gln, and less leaves on media with 16 amino acids except Leu, Phe, Tyr, Asp, Gln and GABA (Supplementary Tables S10, S11). The rosette diameter of ZmAAP4-OE seedlings significantly increased on the medium with Ala and decreased on media with 14 amino acids except Ala, Leu, Thr, His, Asp, Asn, Gln and Cit (Supplementary Tables S9, S11); the rosette diameter of ZmVAAT3-OE seedlings significantly increased on media supplemented with Ala and decreased on media containing Gly, Val, Ile, Ser, Tyr, Trp, His, Lys, Glu, Gln or Cit (Supplementary Tables S10, S11). Not surprisingly, the fresh weight of ZmAAP4-OE seedlings increased on the medium supplemented with Ala or Leu and decreased on media with 17 amino acids except Ala, Leu, Asp, Asn or Gln (Supplementary Tables S9, S11); while the fresh weight of ZmVAAT3-OE seedlings significantly increased on media with Met, Pro, Phe or Asn and decreased on media containing Ile, Ser, Cys, Trp, His, Lys, Arg, Glu, Gln or GABA (Supplementary Tables S10, S11).

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.