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Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Functional characterization of ZmAAP4 and ZmVAAT3 in the 22∆8AA yeast system. The 23344c and 22Δ8AA served as positive and negative controls, respectively, with the (NH4)2SO4 treatment as a control of N source.
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Figure 9: Functional characterization of ZmAAP4 and ZmVAAT3 in the 22∆8AA yeast system. The 23344c and 22Δ8AA served as positive and negative controls, respectively, with the (NH4)2SO4 treatment as a control of N source.

Mentions: To investigate the transport functionality of ZmAAP4 and ZmVAAT3, we cloned each gene into a pDR195 yeast expression vector and transformed into the yeast mutant strain 22Δ8AA, which is deficient in absorbing arginine, aspartate, citrulline, GABA, glutamate or proline. In sharp contrast to the mutant strain 22Δ8AA itself, the mutant complemented with ZmAAP4 displayed a similar and robust growth pattern on the media with proline and glutamate and dramatically weaker growth on the media with citrulline, GABA, aspartate or arginine (Fig. 9); the yeast mutant expressing ZmVAAT3 showed vigorous growth on the six different media specified by the individual amino acid (Fig. 9), indicating that both ZmAAP4 and ZmVAAT3 transported multiple amino acids in the yeast system, although there was a large variation in transport efficiencies.


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Functional characterization of ZmAAP4 and ZmVAAT3 in the 22∆8AA yeast system. The 23344c and 22Δ8AA served as positive and negative controls, respectively, with the (NH4)2SO4 treatment as a control of N source.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588875&req=5

Figure 9: Functional characterization of ZmAAP4 and ZmVAAT3 in the 22∆8AA yeast system. The 23344c and 22Δ8AA served as positive and negative controls, respectively, with the (NH4)2SO4 treatment as a control of N source.
Mentions: To investigate the transport functionality of ZmAAP4 and ZmVAAT3, we cloned each gene into a pDR195 yeast expression vector and transformed into the yeast mutant strain 22Δ8AA, which is deficient in absorbing arginine, aspartate, citrulline, GABA, glutamate or proline. In sharp contrast to the mutant strain 22Δ8AA itself, the mutant complemented with ZmAAP4 displayed a similar and robust growth pattern on the media with proline and glutamate and dramatically weaker growth on the media with citrulline, GABA, aspartate or arginine (Fig. 9); the yeast mutant expressing ZmVAAT3 showed vigorous growth on the six different media specified by the individual amino acid (Fig. 9), indicating that both ZmAAP4 and ZmVAAT3 transported multiple amino acids in the yeast system, although there was a large variation in transport efficiencies.

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.