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Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


RT-qPCR analysis of selected genes that showed response to LN, but not to low P or low K stress.
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Figure 7: RT-qPCR analysis of selected genes that showed response to LN, but not to low P or low K stress.

Mentions: Lastly, LN specifically responsive genes (P<0.01) were defined as those with significantly differential expression under LN compared to control, while having opposite expression patterns or no differential expression under low phosphorus or low potassium. We comparatively analysed expression levels of a subset of genes closely related to C/N metabolism, hormonal signalling, plant development and defence in the ear using RT-qPCR, and identified six genes with specific up-regulation and eight genes with specific down-regulation under LN in the ear (Fig. 7).


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

RT-qPCR analysis of selected genes that showed response to LN, but not to low P or low K stress.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588875&req=5

Figure 7: RT-qPCR analysis of selected genes that showed response to LN, but not to low P or low K stress.
Mentions: Lastly, LN specifically responsive genes (P<0.01) were defined as those with significantly differential expression under LN compared to control, while having opposite expression patterns or no differential expression under low phosphorus or low potassium. We comparatively analysed expression levels of a subset of genes closely related to C/N metabolism, hormonal signalling, plant development and defence in the ear using RT-qPCR, and identified six genes with specific up-regulation and eight genes with specific down-regulation under LN in the ear (Fig. 7).

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.