Limits...
Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Expression pattern of preferentially expressed genes in the cob or florets under LN.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588875&req=5

Figure 6: Expression pattern of preferentially expressed genes in the cob or florets under LN.

Mentions: Further analysing expression of genes mediating C/N metabolism by MapMan visualization showed differential responses of the cob and florets to LN stress (Supplementary Fig. S1; Supplementary Table S8). In the cob, the expression of two genes involved in starch degradation (GRMZM2G34770 and GRMZM2G082034) was repressed. Similarly, methionine adenosyltransferase (GRMZM2G117198) was down-regulated 2.3-fold, although 3-phosphoglycerate dehydrogenase (GRMZM2G073814) was up-regulated 2.5-fold. Genes mediating minor CHO metabolism were differentially regulated in the cob. Hydrolyzing O-glycosyl compound (GRMZM2G340656) mediating raffinose synthesis and trehalose phosphate synthase 11 (GRMZM2G122231) were down-regulated and a trehalose-6-phosphate phosphatase (GRMZM2G174396) was up-regulated 3.2-fold. Likewise, secondary metabolism related genes had differential response to LN in the cob. A flavonoid related gene (GRMZM2G099420) was up-regulated 3.5-fold in the cob under the LN condition and the lignin biosynthesis related gene (GRMZM2G443445) was down-regulated 8.4-fold. Notably, many genes regulating photosynthetic light reactions had substantial down-regulation in the cob (Supplementary Fig. S1A), in contrast to unanimous up-regulation of genes in the same process in florets in response to LN (Supplementary Fig. S1B). Transcription of many genes in growth-related processes such as cell wall-modification enzymes MERISTEM-5 (GRMZM2G392125) and TOUCH4 (AC210669.3_FG001), and regulator of chromosome condensation 1 family genes (GRMZM2G337819 and GRMZM2G302245) were also up-regulated in florets under LN. Genes implicated in nucleotide salvage and nucleotide degradation were down-regulated by LN in florets. Most genes involved in minor CHO metabolism, secondary metabolism and amino acid metabolism, except glutamate synthesis and proline degradation, remained unchanged in their expression levels in florets (Supplementary Fig. S1B). Notably, 49 genes had a presence/absence expression pattern in the cob and/or florets under LN (Fig. 6), which were involved in carbohydrate metabolism, nitrogen metabolism, transport facilitation, transcription regulation and cell rescue and defence.


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Expression pattern of preferentially expressed genes in the cob or florets under LN.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588875&req=5

Figure 6: Expression pattern of preferentially expressed genes in the cob or florets under LN.
Mentions: Further analysing expression of genes mediating C/N metabolism by MapMan visualization showed differential responses of the cob and florets to LN stress (Supplementary Fig. S1; Supplementary Table S8). In the cob, the expression of two genes involved in starch degradation (GRMZM2G34770 and GRMZM2G082034) was repressed. Similarly, methionine adenosyltransferase (GRMZM2G117198) was down-regulated 2.3-fold, although 3-phosphoglycerate dehydrogenase (GRMZM2G073814) was up-regulated 2.5-fold. Genes mediating minor CHO metabolism were differentially regulated in the cob. Hydrolyzing O-glycosyl compound (GRMZM2G340656) mediating raffinose synthesis and trehalose phosphate synthase 11 (GRMZM2G122231) were down-regulated and a trehalose-6-phosphate phosphatase (GRMZM2G174396) was up-regulated 3.2-fold. Likewise, secondary metabolism related genes had differential response to LN in the cob. A flavonoid related gene (GRMZM2G099420) was up-regulated 3.5-fold in the cob under the LN condition and the lignin biosynthesis related gene (GRMZM2G443445) was down-regulated 8.4-fold. Notably, many genes regulating photosynthetic light reactions had substantial down-regulation in the cob (Supplementary Fig. S1A), in contrast to unanimous up-regulation of genes in the same process in florets in response to LN (Supplementary Fig. S1B). Transcription of many genes in growth-related processes such as cell wall-modification enzymes MERISTEM-5 (GRMZM2G392125) and TOUCH4 (AC210669.3_FG001), and regulator of chromosome condensation 1 family genes (GRMZM2G337819 and GRMZM2G302245) were also up-regulated in florets under LN. Genes implicated in nucleotide salvage and nucleotide degradation were down-regulated by LN in florets. Most genes involved in minor CHO metabolism, secondary metabolism and amino acid metabolism, except glutamate synthesis and proline degradation, remained unchanged in their expression levels in florets (Supplementary Fig. S1B). Notably, 49 genes had a presence/absence expression pattern in the cob and/or florets under LN (Fig. 6), which were involved in carbohydrate metabolism, nitrogen metabolism, transport facilitation, transcription regulation and cell rescue and defence.

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.