Limits...
Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Significantly enriched biological process (BP) GO terms in up-regulated (red) and down-regulated (green) genes in (A) the cob versus (B) the florets, under LN.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4588875&req=5

Figure 5: Significantly enriched biological process (BP) GO terms in up-regulated (red) and down-regulated (green) genes in (A) the cob versus (B) the florets, under LN.

Mentions: GO enrichment analysis showed that among 319 genes up-regulated in the cob in response to LN, genes involved in 15 biological processes were enriched; and among 269 genes down-regulated in the cob, genes involved in 14 biological processes were enriched (Fig. 5A). Among 125 genes up-regulated in the florets in response to LN, genes involved in 15 biological processes were enriched; and among 70 genes down-regulated in the florets, genes involved in six biological processes were enriched (Fig. 5B). The most enriched categories in the cob under LN were carbohydrate and nitrogen metabolic processes (Fig. 5A). Although less significant than those in the cob, genes involved in carbohydrate and nitrogen metabolic processes were still enriched in florets (Fig. 5B), indicating that LN affected these two biological processes in both the cob and florets although different number of genes were affected.


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Significantly enriched biological process (BP) GO terms in up-regulated (red) and down-regulated (green) genes in (A) the cob versus (B) the florets, under LN.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588875&req=5

Figure 5: Significantly enriched biological process (BP) GO terms in up-regulated (red) and down-regulated (green) genes in (A) the cob versus (B) the florets, under LN.
Mentions: GO enrichment analysis showed that among 319 genes up-regulated in the cob in response to LN, genes involved in 15 biological processes were enriched; and among 269 genes down-regulated in the cob, genes involved in 14 biological processes were enriched (Fig. 5A). Among 125 genes up-regulated in the florets in response to LN, genes involved in 15 biological processes were enriched; and among 70 genes down-regulated in the florets, genes involved in six biological processes were enriched (Fig. 5B). The most enriched categories in the cob under LN were carbohydrate and nitrogen metabolic processes (Fig. 5A). Although less significant than those in the cob, genes involved in carbohydrate and nitrogen metabolic processes were still enriched in florets (Fig. 5B), indicating that LN affected these two biological processes in both the cob and florets although different number of genes were affected.

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.