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Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Functional categorization of the differentially expressed genes (FDR<0.05) between the cob and florets under sufficient N supply. Each coloured dot represented one gene. Red colour indicates an increased expression in the cob relative to florets whereas the blue colour indicates an increased expression in florets relative to the cob. (A) Metabolism overview of the differentially expressed genes between the cob and florets. (B) Hormone related genes. (C) Cellular response related genes.
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Figure 2: Functional categorization of the differentially expressed genes (FDR<0.05) between the cob and florets under sufficient N supply. Each coloured dot represented one gene. Red colour indicates an increased expression in the cob relative to florets whereas the blue colour indicates an increased expression in florets relative to the cob. (A) Metabolism overview of the differentially expressed genes between the cob and florets. (B) Hormone related genes. (C) Cellular response related genes.

Mentions: Genes involved in metabolic pathways, hormone and cellular response processes were further analysed using the MapMan programme. Strikingly, genes involved in photosynthesis such as chlorophyll binding (photosystem II light harvesting complex gene B1B2 and light-harvesting chlorophyll b-binding protein 3) and photosystem subunit (photosystem II subunit P-2, photosystem I subunit O) genes (Fig. 2A; Supplementary Table S5) were unanimously up-regulated in the cob. Amino acid metabolism related gens GAD2 (Glutamate decarboxylase 2, GRMZM2G355906), a sarcosine oxidase (GRMZM2G052266) and a carboxylyase (GRMZM2G159149) were up-regulated 8.2-, 5.6-, and 10.2-fold, respectively, in the cob (Fig. 2A; Supplementary Table S5). Genes mediating secondary metabolism such as flavonoid and phenylpropanoid metabolism were also generally up-regulated in the cob (Fig. 2A). Up-regulation of these three categories of genes suggested more active C/N metabolism in the cob tissue than in florets at the silking stage. Notably, several genes regulating ABA synthesis and signalling were up-regulated in the cob, indicating that ABA may be an important regulator for cob development during this stage (Fig. 2B). Different from those up-regulated in the cob, a subset of genes regulating cell wall modification, cell wall cellulose synthesis and cell wall degradation were up-regulated in florets (Fig. 2A). Genes involved in lipid degradation such as glycerophosphoryl diester phosphodiesterase (GRMZM2G058- 227) and fatty acid reductase 5 (GRMZM2G120938) were up-regulated 7.2- and 6.7-fold in florets, respectively (Fig. 2A; Supplementary Table S5). In addition, a gene mediating cell division (GRMZM2G166684) was also up-regulated 2.7-fold in florets. Together with enhanced transcription of SWITCH1 (GRMZM2G300786) and cyclin d5;1 (GRMZM2G047637), these results suggested more dynamic cell division, cell wall and membrane formation in florets supporting female flower development in maize (Fig. 2C; Supplementary Table S5).


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Functional categorization of the differentially expressed genes (FDR<0.05) between the cob and florets under sufficient N supply. Each coloured dot represented one gene. Red colour indicates an increased expression in the cob relative to florets whereas the blue colour indicates an increased expression in florets relative to the cob. (A) Metabolism overview of the differentially expressed genes between the cob and florets. (B) Hormone related genes. (C) Cellular response related genes.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588875&req=5

Figure 2: Functional categorization of the differentially expressed genes (FDR<0.05) between the cob and florets under sufficient N supply. Each coloured dot represented one gene. Red colour indicates an increased expression in the cob relative to florets whereas the blue colour indicates an increased expression in florets relative to the cob. (A) Metabolism overview of the differentially expressed genes between the cob and florets. (B) Hormone related genes. (C) Cellular response related genes.
Mentions: Genes involved in metabolic pathways, hormone and cellular response processes were further analysed using the MapMan programme. Strikingly, genes involved in photosynthesis such as chlorophyll binding (photosystem II light harvesting complex gene B1B2 and light-harvesting chlorophyll b-binding protein 3) and photosystem subunit (photosystem II subunit P-2, photosystem I subunit O) genes (Fig. 2A; Supplementary Table S5) were unanimously up-regulated in the cob. Amino acid metabolism related gens GAD2 (Glutamate decarboxylase 2, GRMZM2G355906), a sarcosine oxidase (GRMZM2G052266) and a carboxylyase (GRMZM2G159149) were up-regulated 8.2-, 5.6-, and 10.2-fold, respectively, in the cob (Fig. 2A; Supplementary Table S5). Genes mediating secondary metabolism such as flavonoid and phenylpropanoid metabolism were also generally up-regulated in the cob (Fig. 2A). Up-regulation of these three categories of genes suggested more active C/N metabolism in the cob tissue than in florets at the silking stage. Notably, several genes regulating ABA synthesis and signalling were up-regulated in the cob, indicating that ABA may be an important regulator for cob development during this stage (Fig. 2B). Different from those up-regulated in the cob, a subset of genes regulating cell wall modification, cell wall cellulose synthesis and cell wall degradation were up-regulated in florets (Fig. 2A). Genes involved in lipid degradation such as glycerophosphoryl diester phosphodiesterase (GRMZM2G058- 227) and fatty acid reductase 5 (GRMZM2G120938) were up-regulated 7.2- and 6.7-fold in florets, respectively (Fig. 2A; Supplementary Table S5). In addition, a gene mediating cell division (GRMZM2G166684) was also up-regulated 2.7-fold in florets. Together with enhanced transcription of SWITCH1 (GRMZM2G300786) and cyclin d5;1 (GRMZM2G047637), these results suggested more dynamic cell division, cell wall and membrane formation in florets supporting female flower development in maize (Fig. 2C; Supplementary Table S5).

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.