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Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Significantly enriched GO terms in up-regulated genes in (A) the cob versus (B) the florets, under sufficient N supply.
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Figure 1: Significantly enriched GO terms in up-regulated genes in (A) the cob versus (B) the florets, under sufficient N supply.

Mentions: Using FDR<0.05 and 2-fold change as thresholds, we found 1864 genes that were differentially expressed between the cob and florets at the silking stage: 1314 up-regulated in the cob and 550 up-regulated in florets (Table 1; Supplementary Table S5). The differentially expressed genes between the cob and florets were next subject to gene ontology (GO) term enrichment analysis. The up-regulated genes in the cob were enriched with genes involved in transport facilitation, C/N metabolism, and photosynthesis (Fig. 1A). In contrast, most up-regulated genes in florets were involved in cellular processes and lipid biosynthesis (Fig. 1B). Genes related to biotic responses were enriched in both tissues (Fig. 1).


Asymmetric transcriptomic signatures between the cob and florets in the maize ear under optimal- and low-nitrogen conditions at silking, and functional characterization of amino acid transporters ZmAAP4 and ZmVAAT3.

Pan X, Hasan MM, Li Y, Liao C, Zheng H, Liu R, Li X - J. Exp. Bot. (2015)

Significantly enriched GO terms in up-regulated genes in (A) the cob versus (B) the florets, under sufficient N supply.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4588875&req=5

Figure 1: Significantly enriched GO terms in up-regulated genes in (A) the cob versus (B) the florets, under sufficient N supply.
Mentions: Using FDR<0.05 and 2-fold change as thresholds, we found 1864 genes that were differentially expressed between the cob and florets at the silking stage: 1314 up-regulated in the cob and 550 up-regulated in florets (Table 1; Supplementary Table S5). The differentially expressed genes between the cob and florets were next subject to gene ontology (GO) term enrichment analysis. The up-regulated genes in the cob were enriched with genes involved in transport facilitation, C/N metabolism, and photosynthesis (Fig. 1A). In contrast, most up-regulated genes in florets were involved in cellular processes and lipid biosynthesis (Fig. 1B). Genes related to biotic responses were enriched in both tissues (Fig. 1).

Bottom Line: Fourteen genes responsive specifically to LN provided potential molecular markers for N-efficient maize breeding.We further functionally characterized two newly identified broad-spectrum amino acid transporters, ZmAAP4 and ZmVAAT3, that showed distinct expression patterns in the cob and florets and potentially important roles in amino-N mobilization in the ear.While both proteins could transport various amino acids into yeast or Arabidopsis cells, ZmAAP4 appeared to have higher efficiencies than ZmVAAT3 in transporting seven out of 22 examined amino acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, China Agricultural University, Beijing 100193, China.

No MeSH data available.