Limits...
Expression of angiogenic factors in cryopreserved mouse ovaries after heterotopic autotransplantation.

Choi WJ, Seok JS, Choi IY, Park JK, Shin JK, Lee SA, Paik WY, Lee JH - Obstet Gynecol Sci (2015)

Bottom Line: Revascularization is critical for successful ovarian tissue transplantation.VEGF and angpt-2 protein levels were significantly lower in cryopreserved ovaries of groups II and III than in controls (group I, P<0.05), whereas groups II and III did not differ significantly in this regard.After autotransplantation of cryopreserved ovarian tissue, VEGF and angpt-2 protein levels did not differ significantly by technique but tended to be lower than corresponding levels in controls.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, School of Medicine, Gyeongsang National University, Jinju, Korea. ; Institute of Health Sciences, Gyeongsang National University, Jinju, Korea.

ABSTRACT

Objective: Revascularization is critical for successful ovarian tissue transplantation. Vascular endothelial growth factor (VEGF) and angiopoietin-2 (angpt-2) are the principal mediators of neovascularization. This study was designed to assess VEGF and angpt-2 levels in cryopreserved ovarian tissue after heterotopic autotransplantation.

Methods: Ovarian tissues harvested from ICR mice at 5 to 6 weeks of age were stratified as follows: no cryopreservation (controls, group I); vitrification in VFS-40 (vitrification, group II); and gradual freezing in dimethyl sulfoxide (slow-freezing, group III). Frozen specimens were thawed at room temperature, assaying VEGF and angpt-2 levels 1 week after cryopreservation and 2 weeks after autotransplantation.

Results: VEGF and angpt-2 protein levels were significantly lower in cryopreserved ovaries of groups II and III than in controls (group I, P<0.05), whereas groups II and III did not differ significantly in this regard. After autotransplantation of cryopreserved ovarian tissue, VEGF and angpt-2 protein levels did not differ significantly by technique but tended to be lower than corresponding levels in controls.

Conclusion: Expression of angiogenic factors in ovarian tissue is thought to vary by method of cryopreservation. Our findings indicate that levels of angiogenic factors expressed in cryopreserved ovarian tissue after autotransplantation do not differ appreciably from control levels, regardless of cryopreservation technique.

No MeSH data available.


Related in: MedlinePlus

Autotransplanted mouse ovary: (A) beneath the abdominal wall (arrow), to be assayed after 2 weeks, and (B) on dish with media.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4588844&req=5

Figure 2: Autotransplanted mouse ovary: (A) beneath the abdominal wall (arrow), to be assayed after 2 weeks, and (B) on dish with media.

Mentions: One ovary of each harvested pair served as heterotopic autotransplant, to be done one week after cryopreservation. Following inhalation anesthesia, the animal's abdominal wall was carefully opened, creating space for a graft without breach of peritoneum. Ovaries were affixed to exposed peritoneum using PolySorb 3-0 suture. Two weeks later, the mice were sacrificed and the ovaries were recovered (Fig. 2). Follicles were observed under microscopy, and Western blot analysis was performed.


Expression of angiogenic factors in cryopreserved mouse ovaries after heterotopic autotransplantation.

Choi WJ, Seok JS, Choi IY, Park JK, Shin JK, Lee SA, Paik WY, Lee JH - Obstet Gynecol Sci (2015)

Autotransplanted mouse ovary: (A) beneath the abdominal wall (arrow), to be assayed after 2 weeks, and (B) on dish with media.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588844&req=5

Figure 2: Autotransplanted mouse ovary: (A) beneath the abdominal wall (arrow), to be assayed after 2 weeks, and (B) on dish with media.
Mentions: One ovary of each harvested pair served as heterotopic autotransplant, to be done one week after cryopreservation. Following inhalation anesthesia, the animal's abdominal wall was carefully opened, creating space for a graft without breach of peritoneum. Ovaries were affixed to exposed peritoneum using PolySorb 3-0 suture. Two weeks later, the mice were sacrificed and the ovaries were recovered (Fig. 2). Follicles were observed under microscopy, and Western blot analysis was performed.

Bottom Line: Revascularization is critical for successful ovarian tissue transplantation.VEGF and angpt-2 protein levels were significantly lower in cryopreserved ovaries of groups II and III than in controls (group I, P<0.05), whereas groups II and III did not differ significantly in this regard.After autotransplantation of cryopreserved ovarian tissue, VEGF and angpt-2 protein levels did not differ significantly by technique but tended to be lower than corresponding levels in controls.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, School of Medicine, Gyeongsang National University, Jinju, Korea. ; Institute of Health Sciences, Gyeongsang National University, Jinju, Korea.

ABSTRACT

Objective: Revascularization is critical for successful ovarian tissue transplantation. Vascular endothelial growth factor (VEGF) and angiopoietin-2 (angpt-2) are the principal mediators of neovascularization. This study was designed to assess VEGF and angpt-2 levels in cryopreserved ovarian tissue after heterotopic autotransplantation.

Methods: Ovarian tissues harvested from ICR mice at 5 to 6 weeks of age were stratified as follows: no cryopreservation (controls, group I); vitrification in VFS-40 (vitrification, group II); and gradual freezing in dimethyl sulfoxide (slow-freezing, group III). Frozen specimens were thawed at room temperature, assaying VEGF and angpt-2 levels 1 week after cryopreservation and 2 weeks after autotransplantation.

Results: VEGF and angpt-2 protein levels were significantly lower in cryopreserved ovaries of groups II and III than in controls (group I, P<0.05), whereas groups II and III did not differ significantly in this regard. After autotransplantation of cryopreserved ovarian tissue, VEGF and angpt-2 protein levels did not differ significantly by technique but tended to be lower than corresponding levels in controls.

Conclusion: Expression of angiogenic factors in ovarian tissue is thought to vary by method of cryopreservation. Our findings indicate that levels of angiogenic factors expressed in cryopreserved ovarian tissue after autotransplantation do not differ appreciably from control levels, regardless of cryopreservation technique.

No MeSH data available.


Related in: MedlinePlus