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Placental expression of D6 decoy receptor in preeclampsia.

Cho GJ, Lee ES, Jin HM, Lee JH, Kim YS, Oh MJ, Seol HJ, Hong SC, Kim HJ - Obstet Gynecol Sci (2015)

Bottom Line: The purpose of this study was to investigate the expression of the D6 decoy receptor that can bind chemokines and target them for degradation, resulting in inhibition of inflammation in placentas from preeclamptic and normal pregnancies.The mRNA and protein of D6 decoy receptor were detected in all of placentas from preeclamptic and normal pregnancies.The expression of the D6 decoy receptor in preeclamptic placentas was significantly lower than in normal placentas.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea.

ABSTRACT

Objective: The purpose of this study was to investigate the expression of the D6 decoy receptor that can bind chemokines and target them for degradation, resulting in inhibition of inflammation in placentas from preeclamptic and normal pregnancies.

Methods: The current study was carried out in 35 pregnant women (23 patients with preeclampsia and 12 healthy, normotensive pregnant women) during the third trimester of pregnancy. The expressions of D6 decoy receptor in the placenta were determined with real time reverse transcriptase polymerase chain reaction and western blotting.

Results: The mRNA and protein of D6 decoy receptor were detected in all of placentas from preeclamptic and normal pregnancies. Placental D6 decoy receptor mRNA expression was significantly lower in patients with preeclampsia than in patients with normal pregnancies. Western blot analyses revealed decreased protein expression in cases of preeclampsia.

Conclusion: The expression of the D6 decoy receptor in preeclamptic placentas was significantly lower than in normal placentas. Further studies are needed to clarify the underlying mechanisms that link decreased expression of placental D6 decoy receptor and preeclampsia.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis for protein expression of placental D6 decoy receptor from preeclamptic and normal placentas. (A) Results from a representative experiment. Bands of almost identical intensity for GAPDH are also shown for each experiment. (B) Mean±standard error of the mean of densitometric numbers was calculated by densitometry. Data were analyzed using ANCOVA (analysis of variance with a covariant) with gestational age as covariant. *P<0.05.
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Figure 3: Western blot analysis for protein expression of placental D6 decoy receptor from preeclamptic and normal placentas. (A) Results from a representative experiment. Bands of almost identical intensity for GAPDH are also shown for each experiment. (B) Mean±standard error of the mean of densitometric numbers was calculated by densitometry. Data were analyzed using ANCOVA (analysis of variance with a covariant) with gestational age as covariant. *P<0.05.

Mentions: The results of western blot analyses are presented in Fig. 3. Protein expression of the D6 decoy receptor was detected in placentas from both preeclamptic and normal pregnancies with a band detected at approximately 90 kD. Protein expression of the D6 decoy receptor was lower in placentas from preeclamptic pregnancies when compared to that in normal placentas (Fig. 3A). The data from all placentas are shown in Fig. 3B. Densitometric studies have also shown that protein expression of the D6 decoy receptor was significantly lower in placentas from preeclamptic pregnancies than those in normal placentas (P<0.05) (Fig. 3B).


Placental expression of D6 decoy receptor in preeclampsia.

Cho GJ, Lee ES, Jin HM, Lee JH, Kim YS, Oh MJ, Seol HJ, Hong SC, Kim HJ - Obstet Gynecol Sci (2015)

Western blot analysis for protein expression of placental D6 decoy receptor from preeclamptic and normal placentas. (A) Results from a representative experiment. Bands of almost identical intensity for GAPDH are also shown for each experiment. (B) Mean±standard error of the mean of densitometric numbers was calculated by densitometry. Data were analyzed using ANCOVA (analysis of variance with a covariant) with gestational age as covariant. *P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588836&req=5

Figure 3: Western blot analysis for protein expression of placental D6 decoy receptor from preeclamptic and normal placentas. (A) Results from a representative experiment. Bands of almost identical intensity for GAPDH are also shown for each experiment. (B) Mean±standard error of the mean of densitometric numbers was calculated by densitometry. Data were analyzed using ANCOVA (analysis of variance with a covariant) with gestational age as covariant. *P<0.05.
Mentions: The results of western blot analyses are presented in Fig. 3. Protein expression of the D6 decoy receptor was detected in placentas from both preeclamptic and normal pregnancies with a band detected at approximately 90 kD. Protein expression of the D6 decoy receptor was lower in placentas from preeclamptic pregnancies when compared to that in normal placentas (Fig. 3A). The data from all placentas are shown in Fig. 3B. Densitometric studies have also shown that protein expression of the D6 decoy receptor was significantly lower in placentas from preeclamptic pregnancies than those in normal placentas (P<0.05) (Fig. 3B).

Bottom Line: The purpose of this study was to investigate the expression of the D6 decoy receptor that can bind chemokines and target them for degradation, resulting in inhibition of inflammation in placentas from preeclamptic and normal pregnancies.The mRNA and protein of D6 decoy receptor were detected in all of placentas from preeclamptic and normal pregnancies.The expression of the D6 decoy receptor in preeclamptic placentas was significantly lower than in normal placentas.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea.

ABSTRACT

Objective: The purpose of this study was to investigate the expression of the D6 decoy receptor that can bind chemokines and target them for degradation, resulting in inhibition of inflammation in placentas from preeclamptic and normal pregnancies.

Methods: The current study was carried out in 35 pregnant women (23 patients with preeclampsia and 12 healthy, normotensive pregnant women) during the third trimester of pregnancy. The expressions of D6 decoy receptor in the placenta were determined with real time reverse transcriptase polymerase chain reaction and western blotting.

Results: The mRNA and protein of D6 decoy receptor were detected in all of placentas from preeclamptic and normal pregnancies. Placental D6 decoy receptor mRNA expression was significantly lower in patients with preeclampsia than in patients with normal pregnancies. Western blot analyses revealed decreased protein expression in cases of preeclampsia.

Conclusion: The expression of the D6 decoy receptor in preeclamptic placentas was significantly lower than in normal placentas. Further studies are needed to clarify the underlying mechanisms that link decreased expression of placental D6 decoy receptor and preeclampsia.

No MeSH data available.


Related in: MedlinePlus