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Integrating Multiple Analytical Datasets to Compare Metabolite Profiles of Mouse Colonic-Cecal Contents and Feces.

Zeng H, Grapov D, Jackson MI, Fahrmann J, Fiehn O, Combs GF - Metabolites (2015)

Bottom Line: We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF).Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism.A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces.

View Article: PubMed Central - PubMed

Affiliation: Grand Forks Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Grand Forks, ND 58203, USA. huawei.zeng@ars.usda.gov.

ABSTRACT
The pattern of metabolites produced by the gut microbiome comprises a phenotype indicative of the means by which that microbiome affects the gut. We characterized that phenotype in mice by conducting metabolomic analyses of the colonic-cecal contents, comparing that to the metabolite patterns of feces in order to determine the suitability of fecal specimens as proxies for assessing the metabolic impact of the gut microbiome. We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF). Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism. A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces. These data comprise the first characterization of relationships among metabolites present in the colonic-cecal contents and feces in a healthy mouse model, and shows that feces can be a useful proxy for assessing the pattern of metabolites to which the colonic mucosum is exposed.

No MeSH data available.


Overview of significant differences in metabolite profiles between colonic mucosal, colonic-cecal content and feces sample type comparisons.
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metabolites-05-00489-f001: Overview of significant differences in metabolite profiles between colonic mucosal, colonic-cecal content and feces sample type comparisons.

Mentions: A total of 270 metabolites were identified in the colonic-cecal contents and feces. Of these 251 (93%) metabolites were found in both types of specimen. Only 19 showed metabolites unique patterns (eight found only in colonic-cecal contents; 11 found only in feces) (Figure 1); however, 115 metabolites showed significantly different relative abundances in colonic-cecal contents vs. feces (padj < 0.05) (Figure 1, Table S1). Metabolic pathway enrichment analysis revealed that these 115 metabolites fell into 21 biochemical pathways (as defined by Kyoto Encyclopedia of Genes and Genomes, KEGG [8]), which were mainly related to amino acid, lipid metabolism, rare amino acids, cofactors, vitamins, signaling molecules, nitrogen, energy, and human disease/cancer.


Integrating Multiple Analytical Datasets to Compare Metabolite Profiles of Mouse Colonic-Cecal Contents and Feces.

Zeng H, Grapov D, Jackson MI, Fahrmann J, Fiehn O, Combs GF - Metabolites (2015)

Overview of significant differences in metabolite profiles between colonic mucosal, colonic-cecal content and feces sample type comparisons.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588808&req=5

metabolites-05-00489-f001: Overview of significant differences in metabolite profiles between colonic mucosal, colonic-cecal content and feces sample type comparisons.
Mentions: A total of 270 metabolites were identified in the colonic-cecal contents and feces. Of these 251 (93%) metabolites were found in both types of specimen. Only 19 showed metabolites unique patterns (eight found only in colonic-cecal contents; 11 found only in feces) (Figure 1); however, 115 metabolites showed significantly different relative abundances in colonic-cecal contents vs. feces (padj < 0.05) (Figure 1, Table S1). Metabolic pathway enrichment analysis revealed that these 115 metabolites fell into 21 biochemical pathways (as defined by Kyoto Encyclopedia of Genes and Genomes, KEGG [8]), which were mainly related to amino acid, lipid metabolism, rare amino acids, cofactors, vitamins, signaling molecules, nitrogen, energy, and human disease/cancer.

Bottom Line: We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF).Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism.A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces.

View Article: PubMed Central - PubMed

Affiliation: Grand Forks Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Grand Forks, ND 58203, USA. huawei.zeng@ars.usda.gov.

ABSTRACT
The pattern of metabolites produced by the gut microbiome comprises a phenotype indicative of the means by which that microbiome affects the gut. We characterized that phenotype in mice by conducting metabolomic analyses of the colonic-cecal contents, comparing that to the metabolite patterns of feces in order to determine the suitability of fecal specimens as proxies for assessing the metabolic impact of the gut microbiome. We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF). Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism. A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces. These data comprise the first characterization of relationships among metabolites present in the colonic-cecal contents and feces in a healthy mouse model, and shows that feces can be a useful proxy for assessing the pattern of metabolites to which the colonic mucosum is exposed.

No MeSH data available.