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The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.

Lim HS, Ha H, Shin HK, Jeong SJ - Mol. Cells (2015)

Bottom Line: Saussurea lappa has been reported to possess anti-atopic properties.In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway.In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro.

View Article: PubMed Central - PubMed

Affiliation: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.

ABSTRACT
Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

No MeSH data available.


Related in: MedlinePlus

Effects of five components of S. lappa on the validation of gene expression using real-time RT-PCR in HaCaT cells. The results are normalized as a ratio of the (A) PI3Kca and (B) IL20Rβ mRNA signal to the GAPDH gene signal within the same sample, and the values expressed. Values are expressed as mean ± SEM of three independent experiments. ##P < 0.01 vs. vehicle control cells; **P < 0.01 vs. TI-treated cells.
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f5-molce-38-9-765: Effects of five components of S. lappa on the validation of gene expression using real-time RT-PCR in HaCaT cells. The results are normalized as a ratio of the (A) PI3Kca and (B) IL20Rβ mRNA signal to the GAPDH gene signal within the same sample, and the values expressed. Values are expressed as mean ± SEM of three independent experiments. ##P < 0.01 vs. vehicle control cells; **P < 0.01 vs. TI-treated cells.

Mentions: To examine the downregulatory effects of PI3Kca, and IL20Rβ in S. lappa-treated in HaCaT cells, the cells were treated with tumor necrosis factor alpha (TNF-α) and IFN-γ in the absence or presence of S. lappa extract. As shown in Figure 3, S. lappa had no significant effect on PI3Kca or IL20Rβ expression in TNF-α/IFN-γ stimulated HaCaT cells. However, five components of S. lappa suppressed PI3Kca mRNA expression in TNF-α/IFN-γ-stimulated HaCaT cells. Among the five components of S. lappa, costic acid and dehydrocostus lactone suppressed PI3Kca mRNA expression in a dose-dependent manner (Fig. 4). By contrast, no inhibitory effect of these components on IL20Rβ expression was observed in TNF-α/IFN-γ-stimulated HaCaT cells (Fig. 5).


The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.

Lim HS, Ha H, Shin HK, Jeong SJ - Mol. Cells (2015)

Effects of five components of S. lappa on the validation of gene expression using real-time RT-PCR in HaCaT cells. The results are normalized as a ratio of the (A) PI3Kca and (B) IL20Rβ mRNA signal to the GAPDH gene signal within the same sample, and the values expressed. Values are expressed as mean ± SEM of three independent experiments. ##P < 0.01 vs. vehicle control cells; **P < 0.01 vs. TI-treated cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588719&req=5

f5-molce-38-9-765: Effects of five components of S. lappa on the validation of gene expression using real-time RT-PCR in HaCaT cells. The results are normalized as a ratio of the (A) PI3Kca and (B) IL20Rβ mRNA signal to the GAPDH gene signal within the same sample, and the values expressed. Values are expressed as mean ± SEM of three independent experiments. ##P < 0.01 vs. vehicle control cells; **P < 0.01 vs. TI-treated cells.
Mentions: To examine the downregulatory effects of PI3Kca, and IL20Rβ in S. lappa-treated in HaCaT cells, the cells were treated with tumor necrosis factor alpha (TNF-α) and IFN-γ in the absence or presence of S. lappa extract. As shown in Figure 3, S. lappa had no significant effect on PI3Kca or IL20Rβ expression in TNF-α/IFN-γ stimulated HaCaT cells. However, five components of S. lappa suppressed PI3Kca mRNA expression in TNF-α/IFN-γ-stimulated HaCaT cells. Among the five components of S. lappa, costic acid and dehydrocostus lactone suppressed PI3Kca mRNA expression in a dose-dependent manner (Fig. 4). By contrast, no inhibitory effect of these components on IL20Rβ expression was observed in TNF-α/IFN-γ-stimulated HaCaT cells (Fig. 5).

Bottom Line: Saussurea lappa has been reported to possess anti-atopic properties.In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway.In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro.

View Article: PubMed Central - PubMed

Affiliation: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.

ABSTRACT
Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

No MeSH data available.


Related in: MedlinePlus