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The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.

Lim HS, Ha H, Shin HK, Jeong SJ - Mol. Cells (2015)

Bottom Line: Saussurea lappa has been reported to possess anti-atopic properties.In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway.In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro.

View Article: PubMed Central - PubMed

Affiliation: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.

ABSTRACT
Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

No MeSH data available.


Related in: MedlinePlus

Validation of selected up-regulated genes using real-time qPCR in skin tissue from Nc/Nga mice. The results are normalized as a ratio of each specific mRNA signal (KDR, IL13Rα2, IFNα1, TSLP, and CXCL11) to the β-actin gene signal within the same sample and the values expressed. Data are presented as mean ± SD (n = 3). #p < 0.05 and ##p < 0.01 vs. normal control.
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f2-molce-38-9-765: Validation of selected up-regulated genes using real-time qPCR in skin tissue from Nc/Nga mice. The results are normalized as a ratio of each specific mRNA signal (KDR, IL13Rα2, IFNα1, TSLP, and CXCL11) to the β-actin gene signal within the same sample and the values expressed. Data are presented as mean ± SD (n = 3). #p < 0.05 and ##p < 0.01 vs. normal control.

Mentions: To confirm the effects of S. lappa extract on AD metabolism genes at the mRNA level, real-time RT-PCR was conducted for 10 selected genes involved in cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the results of the microarray analysis, S. lappa extract increased the expression of kinase insert domain protein receptor (KDR), interleukin 13 receptor alpha 2 (IL13Rα2), interferon alpha 1 (IFNα1), thymic stromal lymphopoietin (TSLP), and chemokine (C-X-C motif) ligand 11 (CXCL11) in the Biostir group (Fig. 2). In addition, S. lappa extract suppressed the expression of chemokine (C-C motif) receptor 1 (CCR1), interleukin 2 receptor beta (IL2Rβ), phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3Kca), and interleukin 20 receptor beta (IL20Rβ) in the Biostir group (Fig. 3). Among the downregulated genes, S. lappa significantly suppressed PI3Kca and IL20Rβ. The relative expression levels of each gene were normalized relative to the expression of β-actin, a well-known housekeeping gene.


The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.

Lim HS, Ha H, Shin HK, Jeong SJ - Mol. Cells (2015)

Validation of selected up-regulated genes using real-time qPCR in skin tissue from Nc/Nga mice. The results are normalized as a ratio of each specific mRNA signal (KDR, IL13Rα2, IFNα1, TSLP, and CXCL11) to the β-actin gene signal within the same sample and the values expressed. Data are presented as mean ± SD (n = 3). #p < 0.05 and ##p < 0.01 vs. normal control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588719&req=5

f2-molce-38-9-765: Validation of selected up-regulated genes using real-time qPCR in skin tissue from Nc/Nga mice. The results are normalized as a ratio of each specific mRNA signal (KDR, IL13Rα2, IFNα1, TSLP, and CXCL11) to the β-actin gene signal within the same sample and the values expressed. Data are presented as mean ± SD (n = 3). #p < 0.05 and ##p < 0.01 vs. normal control.
Mentions: To confirm the effects of S. lappa extract on AD metabolism genes at the mRNA level, real-time RT-PCR was conducted for 10 selected genes involved in cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the results of the microarray analysis, S. lappa extract increased the expression of kinase insert domain protein receptor (KDR), interleukin 13 receptor alpha 2 (IL13Rα2), interferon alpha 1 (IFNα1), thymic stromal lymphopoietin (TSLP), and chemokine (C-X-C motif) ligand 11 (CXCL11) in the Biostir group (Fig. 2). In addition, S. lappa extract suppressed the expression of chemokine (C-C motif) receptor 1 (CCR1), interleukin 2 receptor beta (IL2Rβ), phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3Kca), and interleukin 20 receptor beta (IL20Rβ) in the Biostir group (Fig. 3). Among the downregulated genes, S. lappa significantly suppressed PI3Kca and IL20Rβ. The relative expression levels of each gene were normalized relative to the expression of β-actin, a well-known housekeeping gene.

Bottom Line: Saussurea lappa has been reported to possess anti-atopic properties.In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway.In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro.

View Article: PubMed Central - PubMed

Affiliation: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.

ABSTRACT
Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

No MeSH data available.


Related in: MedlinePlus