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The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.

Lim HS, Ha H, Shin HK, Jeong SJ - Mol. Cells (2015)

Bottom Line: Saussurea lappa has been reported to possess anti-atopic properties.In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway.In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro.

View Article: PubMed Central - PubMed

Affiliation: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.

ABSTRACT
Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

No MeSH data available.


Related in: MedlinePlus

Gene ontology assignment of differentially expressed genes. Clustergram of up- and down-regulated genes in skin tissue from Nc/Nga mice. Microarray data from control (untreated cells) and experimental groups (300 mg/kg S. lappa-treated group) were combined and clustered. (A) Each gene is represented by a single row of clustered boxes and each group is represented by a single column. (B) Gene ontology classification based on biological processes.
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f1-molce-38-9-765: Gene ontology assignment of differentially expressed genes. Clustergram of up- and down-regulated genes in skin tissue from Nc/Nga mice. Microarray data from control (untreated cells) and experimental groups (300 mg/kg S. lappa-treated group) were combined and clustered. (A) Each gene is represented by a single row of clustered boxes and each group is represented by a single column. (B) Gene ontology classification based on biological processes.

Mentions: Gene expression profiles were significantly up or downregulated in the S. lappa extract group compared with the Biostir group. A total of 4255 genes that were differentially expressed from about 24,000 genes detected in the experimental group. A hierarchical clustering algorithm was used to group the genes based on their similar expression patterns. Gene ontology annotation was achieved using the KEGG database and the genes were placed into 173 biological pathways (Supplementary Table 2): MAPK signaling pathway (48 genes), neuroactive ligand-receptor interaction (31 genes), glycan structures biosynthesis 1 (29 genes), cytokine-cytokine receptor interaction (29 genes), calcium signaling pathway (26 genes), Wnt signaling pathway (25 genes), regulation of actin cytoskeleton (25 genes), oxidative phosphorylation (25 genes), JAK-STAT signaling pathway (24 genes), focal adhesion (23 genes), tight junction (22 genes), purine metabolism (23 genes), cell cycle (21 genes), leukocyte transendothelial migration (20 genes), and cell adhesion molecules (20 genes) (Fig. 1). Among the 173 biological pathways that were up or downregulated, we selected five upregulated and six downregulated allergy-related pathways. The functionally annotated upregulated (40) and downregulated (45) genes are listed in Supplementary Tables 3 and 4, which show comparisons of the expression levels for a variety of genes between the Biostir and S. lappa group.


The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.

Lim HS, Ha H, Shin HK, Jeong SJ - Mol. Cells (2015)

Gene ontology assignment of differentially expressed genes. Clustergram of up- and down-regulated genes in skin tissue from Nc/Nga mice. Microarray data from control (untreated cells) and experimental groups (300 mg/kg S. lappa-treated group) were combined and clustered. (A) Each gene is represented by a single row of clustered boxes and each group is represented by a single column. (B) Gene ontology classification based on biological processes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588719&req=5

f1-molce-38-9-765: Gene ontology assignment of differentially expressed genes. Clustergram of up- and down-regulated genes in skin tissue from Nc/Nga mice. Microarray data from control (untreated cells) and experimental groups (300 mg/kg S. lappa-treated group) were combined and clustered. (A) Each gene is represented by a single row of clustered boxes and each group is represented by a single column. (B) Gene ontology classification based on biological processes.
Mentions: Gene expression profiles were significantly up or downregulated in the S. lappa extract group compared with the Biostir group. A total of 4255 genes that were differentially expressed from about 24,000 genes detected in the experimental group. A hierarchical clustering algorithm was used to group the genes based on their similar expression patterns. Gene ontology annotation was achieved using the KEGG database and the genes were placed into 173 biological pathways (Supplementary Table 2): MAPK signaling pathway (48 genes), neuroactive ligand-receptor interaction (31 genes), glycan structures biosynthesis 1 (29 genes), cytokine-cytokine receptor interaction (29 genes), calcium signaling pathway (26 genes), Wnt signaling pathway (25 genes), regulation of actin cytoskeleton (25 genes), oxidative phosphorylation (25 genes), JAK-STAT signaling pathway (24 genes), focal adhesion (23 genes), tight junction (22 genes), purine metabolism (23 genes), cell cycle (21 genes), leukocyte transendothelial migration (20 genes), and cell adhesion molecules (20 genes) (Fig. 1). Among the 173 biological pathways that were up or downregulated, we selected five upregulated and six downregulated allergy-related pathways. The functionally annotated upregulated (40) and downregulated (45) genes are listed in Supplementary Tables 3 and 4, which show comparisons of the expression levels for a variety of genes between the Biostir and S. lappa group.

Bottom Line: Saussurea lappa has been reported to possess anti-atopic properties.In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway.In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro.

View Article: PubMed Central - PubMed

Affiliation: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.

ABSTRACT
Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

No MeSH data available.


Related in: MedlinePlus