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Diminished Resistance to Hyperoxia in Brains of Reproductively Senescent Female CBA/H Mice.

Šarić A, Sobočanec S, Mačak Šafranko Ž, Popović Hadžija M, Bagarić R, Farkaš V, Švarc A, Marotti T, Balog T - Med Sci Monit Basic Res (2015)

Bottom Line: Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023).Uptake of 18FDG was decreased after hyperoxia in the back brain of females.CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia.

ABSTRACT
BACKGROUND We have explored sex differences in ability to maintain redox balance during acute oxidative stress in brains of mice. We aimed to determine if there were differences in oxidative/antioxidative status upon hyperoxia in brains of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. MATERIAL AND METHODS The brains of 12-month-old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18-h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx-1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. RESULTS No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the back brain of females. CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.

No MeSH data available.


Related in: MedlinePlus

Graphical display of glucose consumption in back brain vs. whole brain of CBA/H mice, expressed as a percentage of SUV ratio. Bars represent mean ±SD; significant difference in back brain glucose consumption is observed between male and female normoxia-treated mice (** p=0.008), and between female normoxia and hyperoxia-treated mice (*** p<0.001), respectively.
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f5-medscimonitbasicres-21-191: Graphical display of glucose consumption in back brain vs. whole brain of CBA/H mice, expressed as a percentage of SUV ratio. Bars represent mean ±SD; significant difference in back brain glucose consumption is observed between male and female normoxia-treated mice (** p=0.008), and between female normoxia and hyperoxia-treated mice (*** p<0.001), respectively.

Mentions: Graphical display of glucose consumption in back brain vs. whole brain of CBA/H mice, expressed as a percentage of SUV ratio is presented in Figure 5. The percentage of total SUV in back brain of female control mice was 8% higher in relation to their corresponding male controls (p=0.008). Moreover, glucose consumption in the back brain of hyperoxia-treated females was significantly lower, when compared to normoxia-treated females (p<0.001). In males, we found no difference in 18FDG uptake in back brain between normoxia and hyperoxia-treated group of animals.


Diminished Resistance to Hyperoxia in Brains of Reproductively Senescent Female CBA/H Mice.

Šarić A, Sobočanec S, Mačak Šafranko Ž, Popović Hadžija M, Bagarić R, Farkaš V, Švarc A, Marotti T, Balog T - Med Sci Monit Basic Res (2015)

Graphical display of glucose consumption in back brain vs. whole brain of CBA/H mice, expressed as a percentage of SUV ratio. Bars represent mean ±SD; significant difference in back brain glucose consumption is observed between male and female normoxia-treated mice (** p=0.008), and between female normoxia and hyperoxia-treated mice (*** p<0.001), respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4588673&req=5

f5-medscimonitbasicres-21-191: Graphical display of glucose consumption in back brain vs. whole brain of CBA/H mice, expressed as a percentage of SUV ratio. Bars represent mean ±SD; significant difference in back brain glucose consumption is observed between male and female normoxia-treated mice (** p=0.008), and between female normoxia and hyperoxia-treated mice (*** p<0.001), respectively.
Mentions: Graphical display of glucose consumption in back brain vs. whole brain of CBA/H mice, expressed as a percentage of SUV ratio is presented in Figure 5. The percentage of total SUV in back brain of female control mice was 8% higher in relation to their corresponding male controls (p=0.008). Moreover, glucose consumption in the back brain of hyperoxia-treated females was significantly lower, when compared to normoxia-treated females (p<0.001). In males, we found no difference in 18FDG uptake in back brain between normoxia and hyperoxia-treated group of animals.

Bottom Line: Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023).Uptake of 18FDG was decreased after hyperoxia in the back brain of females.CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia.

ABSTRACT
BACKGROUND We have explored sex differences in ability to maintain redox balance during acute oxidative stress in brains of mice. We aimed to determine if there were differences in oxidative/antioxidative status upon hyperoxia in brains of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. MATERIAL AND METHODS The brains of 12-month-old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18-h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx-1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. RESULTS No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the back brain of females. CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.

No MeSH data available.


Related in: MedlinePlus