Limits...
Diminished Resistance to Hyperoxia in Brains of Reproductively Senescent Female CBA/H Mice.

Šarić A, Sobočanec S, Mačak Šafranko Ž, Popović Hadžija M, Bagarić R, Farkaš V, Švarc A, Marotti T, Balog T - Med Sci Monit Basic Res (2015)

Bottom Line: Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023).Uptake of 18FDG was decreased after hyperoxia in the back brain of females.CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia.

ABSTRACT
BACKGROUND We have explored sex differences in ability to maintain redox balance during acute oxidative stress in brains of mice. We aimed to determine if there were differences in oxidative/antioxidative status upon hyperoxia in brains of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. MATERIAL AND METHODS The brains of 12-month-old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18-h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx-1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. RESULTS No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the back brain of females. CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.

No MeSH data available.


Related in: MedlinePlus

Representative PET mouse brain images using 18FDG as a marker, visualized and ordered as follows: (A) female control; (B) male control; (C) female hyperoxia; (D) male hyperoxia. The bars represent calculated SUV (g/ml) data of PET mouse brain image co-registered with CT mouse brain template. Region of interest (back brain) is selected. Harderian glands are also annotated. Harderian glands are physiologically highly visible because of their ability to incorporate 18FDG from the blood more avidly than any other tissue.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4588673&req=5

f4-medscimonitbasicres-21-191: Representative PET mouse brain images using 18FDG as a marker, visualized and ordered as follows: (A) female control; (B) male control; (C) female hyperoxia; (D) male hyperoxia. The bars represent calculated SUV (g/ml) data of PET mouse brain image co-registered with CT mouse brain template. Region of interest (back brain) is selected. Harderian glands are also annotated. Harderian glands are physiologically highly visible because of their ability to incorporate 18FDG from the blood more avidly than any other tissue.

Mentions: In order to determine if there was any difference between glucose metabolism in brain of CBA/H mice with respect to sex and/or treatment, we measured brain glucose uptake. The representative pictures of all groups using 18FDG as a marker are presented in Figure 4 (A – female control, B – male control, C – female hyperoxia, D – male hyperoxia).


Diminished Resistance to Hyperoxia in Brains of Reproductively Senescent Female CBA/H Mice.

Šarić A, Sobočanec S, Mačak Šafranko Ž, Popović Hadžija M, Bagarić R, Farkaš V, Švarc A, Marotti T, Balog T - Med Sci Monit Basic Res (2015)

Representative PET mouse brain images using 18FDG as a marker, visualized and ordered as follows: (A) female control; (B) male control; (C) female hyperoxia; (D) male hyperoxia. The bars represent calculated SUV (g/ml) data of PET mouse brain image co-registered with CT mouse brain template. Region of interest (back brain) is selected. Harderian glands are also annotated. Harderian glands are physiologically highly visible because of their ability to incorporate 18FDG from the blood more avidly than any other tissue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4588673&req=5

f4-medscimonitbasicres-21-191: Representative PET mouse brain images using 18FDG as a marker, visualized and ordered as follows: (A) female control; (B) male control; (C) female hyperoxia; (D) male hyperoxia. The bars represent calculated SUV (g/ml) data of PET mouse brain image co-registered with CT mouse brain template. Region of interest (back brain) is selected. Harderian glands are also annotated. Harderian glands are physiologically highly visible because of their ability to incorporate 18FDG from the blood more avidly than any other tissue.
Mentions: In order to determine if there was any difference between glucose metabolism in brain of CBA/H mice with respect to sex and/or treatment, we measured brain glucose uptake. The representative pictures of all groups using 18FDG as a marker are presented in Figure 4 (A – female control, B – male control, C – female hyperoxia, D – male hyperoxia).

Bottom Line: Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023).Uptake of 18FDG was decreased after hyperoxia in the back brain of females.CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia.

ABSTRACT
BACKGROUND We have explored sex differences in ability to maintain redox balance during acute oxidative stress in brains of mice. We aimed to determine if there were differences in oxidative/antioxidative status upon hyperoxia in brains of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. MATERIAL AND METHODS The brains of 12-month-old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18-h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx-1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. RESULTS No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the back brain of females. CONCLUSIONS We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.

No MeSH data available.


Related in: MedlinePlus