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Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism.

Wang N, Wei RB, Li QP, Yang X, Li P, Huang MJ, Wang R, Cai GY, Chen XM - Med. Sci. Monit. (2015)

Bottom Line: Both the NAC and probucol groups demonstrated significantly lower Scr, BUN, and urine protein levels compared to the model group (P<0.05), with no significant difference between these 2 groups.Probucol can effectively reduce kidney damage caused by contrast agent.The underlying mechanism may be that probucol accelerates the recovery of renal function and renal pathology by reducing local renal oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, China (mainland).

ABSTRACT

Background: Contrast-induced nephropathy (CIN) refers to acute renal damage that occurs after the use of contrast agents. This study investigated the renal protective effect of probucol in a rat model of contrast-induced nephropathy and the mechanism of its effect.

Material and methods: Twenty-eight Wistar rats were randomly divided into the control group, model group, N-acetylcysteine(NAC) group, and probucol group. We used a rat model of iopromide-induced CIN. One day prior to modeling, the rats received gavage. At 24 h after the modeling, blood biochemistry and urine protein were assessed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured in renal tissue. Kidney sections were created for histopathological examination.

Results: The model group of rats showed significantly elevated levels of blood creatinine, urea nitrogen, 24-h urine protein, histopathological scores, and parameters of oxidative stress (P<0.05). Both the NAC and probucol groups demonstrated significantly lower Scr, BUN, and urine protein levels compared to the model group (P<0.05), with no significant difference between these 2 groups. The NAC group and the probucol group had significantly lower MDA and higher SOD than the model group at 24 h after modeling (P<0.05). The 8-OHdG-positive tubule of the probucol group and NAC group were significantly lower than those of the model group (p=0.046, P=0.0008), with significant difference between these 2 groups (P=0.024).

Conclusions: Probucol can effectively reduce kidney damage caused by contrast agent. The underlying mechanism may be that probucol accelerates the recovery of renal function and renal pathology by reducing local renal oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Tubular damage scores of rats after modeling 24 h. ## P<0.01, compared with the control group.* P<0.05, compared with the model group.
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f3-medscimonit-21-2886: Tubular damage scores of rats after modeling 24 h. ## P<0.01, compared with the control group.* P<0.05, compared with the model group.

Mentions: PAS staining of kidney tissues showed that the renal tubular epithelial cells of the control group presented a normal morphology and structure, without any luminal expansion or urinary casts. However, at 24 h after the procedure, the model group showed obvious tubular epithelial vacuolar degeneration and disintegration and shedding of the brush border, as well as visible cell casts and protein casts in regions of the lumen. In comparison, the probucol group showed milder pathological changes than the model group, but still presented fairly obvious vacuolar degeneration and brush border loss, and the NAC group showed milder pathological changes than the probucol group (Figures 2, 3). Electron microscopy showed that the mitochondria of renal tubular epithelial cells in the model group were swollen and had ridge fractures. The NAC group and probucol mitochondrial group showed milder mitochondria swelling compared with the model group (Figure 4).


Renal Protective Effect of Probucol in Rats with Contrast-Induced Nephropathy and its Underlying Mechanism.

Wang N, Wei RB, Li QP, Yang X, Li P, Huang MJ, Wang R, Cai GY, Chen XM - Med. Sci. Monit. (2015)

Tubular damage scores of rats after modeling 24 h. ## P<0.01, compared with the control group.* P<0.05, compared with the model group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4588667&req=5

f3-medscimonit-21-2886: Tubular damage scores of rats after modeling 24 h. ## P<0.01, compared with the control group.* P<0.05, compared with the model group.
Mentions: PAS staining of kidney tissues showed that the renal tubular epithelial cells of the control group presented a normal morphology and structure, without any luminal expansion or urinary casts. However, at 24 h after the procedure, the model group showed obvious tubular epithelial vacuolar degeneration and disintegration and shedding of the brush border, as well as visible cell casts and protein casts in regions of the lumen. In comparison, the probucol group showed milder pathological changes than the model group, but still presented fairly obvious vacuolar degeneration and brush border loss, and the NAC group showed milder pathological changes than the probucol group (Figures 2, 3). Electron microscopy showed that the mitochondria of renal tubular epithelial cells in the model group were swollen and had ridge fractures. The NAC group and probucol mitochondrial group showed milder mitochondria swelling compared with the model group (Figure 4).

Bottom Line: Both the NAC and probucol groups demonstrated significantly lower Scr, BUN, and urine protein levels compared to the model group (P<0.05), with no significant difference between these 2 groups.Probucol can effectively reduce kidney damage caused by contrast agent.The underlying mechanism may be that probucol accelerates the recovery of renal function and renal pathology by reducing local renal oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, China (mainland).

ABSTRACT

Background: Contrast-induced nephropathy (CIN) refers to acute renal damage that occurs after the use of contrast agents. This study investigated the renal protective effect of probucol in a rat model of contrast-induced nephropathy and the mechanism of its effect.

Material and methods: Twenty-eight Wistar rats were randomly divided into the control group, model group, N-acetylcysteine(NAC) group, and probucol group. We used a rat model of iopromide-induced CIN. One day prior to modeling, the rats received gavage. At 24 h after the modeling, blood biochemistry and urine protein were assessed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured in renal tissue. Kidney sections were created for histopathological examination.

Results: The model group of rats showed significantly elevated levels of blood creatinine, urea nitrogen, 24-h urine protein, histopathological scores, and parameters of oxidative stress (P<0.05). Both the NAC and probucol groups demonstrated significantly lower Scr, BUN, and urine protein levels compared to the model group (P<0.05), with no significant difference between these 2 groups. The NAC group and the probucol group had significantly lower MDA and higher SOD than the model group at 24 h after modeling (P<0.05). The 8-OHdG-positive tubule of the probucol group and NAC group were significantly lower than those of the model group (p=0.046, P=0.0008), with significant difference between these 2 groups (P=0.024).

Conclusions: Probucol can effectively reduce kidney damage caused by contrast agent. The underlying mechanism may be that probucol accelerates the recovery of renal function and renal pathology by reducing local renal oxidative stress.

No MeSH data available.


Related in: MedlinePlus