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Intra-Bone Marrow Transplantation of Endosteal Bone Marrow Cells Facilitates Allogeneic Hematopoietic and Stromal Cells Engraftment Dependent on Early Expression of CXCL-12.

Chen C, Su Y, Chen J, Zhang D, Song Y, Guo S - Med. Sci. Monit. (2015)

Bottom Line: We compared the new method with each control group for allogeneic HSCs homing pattern, peripheral blood chimerism level, skin allograft survival time, and donor stromal cell percentage in recipient BM.By AMD3100 blockade at day 1, peripheral blood chimerism level and donor stromal cell percentage were significantly reduced as compared to the control group without AMD3100 blockade.Our study suggests that IBBMT of endosteal BMCs is an effective approach for HSCT in inducing allogeneic hematopoietic reconstitution.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Reconstructive Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).

ABSTRACT

Background: Hematopoietic stem cell transplantation (HSCT) has been considered as an effective approach at inducing allogeneic hematopoietic reconstitution and immune tolerance. However, it remains critical to find the optimal HSCT delivery method and robust sources of hematopoietic stem cells (HSCs).

Material and methods: We introduced a new method by infusing allogeneic endosteal bone marrow cells (BMCs) harvested from long bones endosteum through intra-bone marrow transplantation (IBBMT) into irradiated mice. Recipient mice that were transplanted with central BMCs or through intravenous bone marrow transplantation (IVBMT) were used as controls (n=6 per group). We compared the new method with each control group for allogeneic HSCs homing pattern, peripheral blood chimerism level, skin allograft survival time, and donor stromal cell percentage in recipient BM. AMD3100 was injected to determine whether chemokine stromal cell-derived factor-1 (CXCL-12) was critical for the new method.

Results: More allogeneic HSCs homed into spleen and bone marrow for the new method as compared to each control group. IBBMT of endosteal BMCs led to a higher peripheral blood chimerism and skin allograft survival. At 18 weeks, donor stromal cell percentage in recipient BMCs was higher for the new method than in each control group. By AMD3100 blockade at day 1, peripheral blood chimerism level and donor stromal cell percentage were significantly reduced as compared to the control group without AMD3100 blockade.

Conclusions: Our study suggests that IBBMT of endosteal BMCs is an effective approach for HSCT in inducing allogeneic hematopoietic reconstitution. The advantage is dependent upon the early expression of CXCL-12 after bone marrow transplantation.

No MeSH data available.


Related in: MedlinePlus

Representative data of homing assay analyzes. CFSE-labeled donor HSCs were injected into unirradiated recipient mice at 0-day. Recipient mice were sacrificed 24 hours later for CFSE-positive cell percentage in thymus, spleen, bone marrows (injected BM and non-injected BM, respectively, for IB-eBMCs and IB-cBMCs group). CFSE-positive cell percentage was calculated as CFSE positive cells in CD45+ cells. Each diagram represents the CFSE-positive cell percentage data of 6 mice for 1 specific location.
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f2-medscimonit-21-2757: Representative data of homing assay analyzes. CFSE-labeled donor HSCs were injected into unirradiated recipient mice at 0-day. Recipient mice were sacrificed 24 hours later for CFSE-positive cell percentage in thymus, spleen, bone marrows (injected BM and non-injected BM, respectively, for IB-eBMCs and IB-cBMCs group). CFSE-positive cell percentage was calculated as CFSE positive cells in CD45+ cells. Each diagram represents the CFSE-positive cell percentage data of 6 mice for 1 specific location.

Mentions: The previous work by Haylock [13] showed that HSCs from endosteal region had a higher capacity of homing into recipient BMs. To determine whether IBBMT delivery of endosteal BMCs improved donor HSCs homing, we performed a homing assay by tracking CFSE-labeled allogeneic HSCs. We found that more donor-derived cells in the IB-eBMCs group than in either control group homed in spleen and BMs (both the injected and non-injected BM) but not in thymus (Table 2, Figure 2). Of note, the frequency of donor-derived cells was highest in the injected BM for both IB-eBMCs and IB-cBMCs groups. IBBMT of endosteal BMCs had a higher efficiency of homing into recipient lymphoid organ and BMs.


Intra-Bone Marrow Transplantation of Endosteal Bone Marrow Cells Facilitates Allogeneic Hematopoietic and Stromal Cells Engraftment Dependent on Early Expression of CXCL-12.

Chen C, Su Y, Chen J, Zhang D, Song Y, Guo S - Med. Sci. Monit. (2015)

Representative data of homing assay analyzes. CFSE-labeled donor HSCs were injected into unirradiated recipient mice at 0-day. Recipient mice were sacrificed 24 hours later for CFSE-positive cell percentage in thymus, spleen, bone marrows (injected BM and non-injected BM, respectively, for IB-eBMCs and IB-cBMCs group). CFSE-positive cell percentage was calculated as CFSE positive cells in CD45+ cells. Each diagram represents the CFSE-positive cell percentage data of 6 mice for 1 specific location.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4588631&req=5

f2-medscimonit-21-2757: Representative data of homing assay analyzes. CFSE-labeled donor HSCs were injected into unirradiated recipient mice at 0-day. Recipient mice were sacrificed 24 hours later for CFSE-positive cell percentage in thymus, spleen, bone marrows (injected BM and non-injected BM, respectively, for IB-eBMCs and IB-cBMCs group). CFSE-positive cell percentage was calculated as CFSE positive cells in CD45+ cells. Each diagram represents the CFSE-positive cell percentage data of 6 mice for 1 specific location.
Mentions: The previous work by Haylock [13] showed that HSCs from endosteal region had a higher capacity of homing into recipient BMs. To determine whether IBBMT delivery of endosteal BMCs improved donor HSCs homing, we performed a homing assay by tracking CFSE-labeled allogeneic HSCs. We found that more donor-derived cells in the IB-eBMCs group than in either control group homed in spleen and BMs (both the injected and non-injected BM) but not in thymus (Table 2, Figure 2). Of note, the frequency of donor-derived cells was highest in the injected BM for both IB-eBMCs and IB-cBMCs groups. IBBMT of endosteal BMCs had a higher efficiency of homing into recipient lymphoid organ and BMs.

Bottom Line: We compared the new method with each control group for allogeneic HSCs homing pattern, peripheral blood chimerism level, skin allograft survival time, and donor stromal cell percentage in recipient BM.By AMD3100 blockade at day 1, peripheral blood chimerism level and donor stromal cell percentage were significantly reduced as compared to the control group without AMD3100 blockade.Our study suggests that IBBMT of endosteal BMCs is an effective approach for HSCT in inducing allogeneic hematopoietic reconstitution.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Reconstructive Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).

ABSTRACT

Background: Hematopoietic stem cell transplantation (HSCT) has been considered as an effective approach at inducing allogeneic hematopoietic reconstitution and immune tolerance. However, it remains critical to find the optimal HSCT delivery method and robust sources of hematopoietic stem cells (HSCs).

Material and methods: We introduced a new method by infusing allogeneic endosteal bone marrow cells (BMCs) harvested from long bones endosteum through intra-bone marrow transplantation (IBBMT) into irradiated mice. Recipient mice that were transplanted with central BMCs or through intravenous bone marrow transplantation (IVBMT) were used as controls (n=6 per group). We compared the new method with each control group for allogeneic HSCs homing pattern, peripheral blood chimerism level, skin allograft survival time, and donor stromal cell percentage in recipient BM. AMD3100 was injected to determine whether chemokine stromal cell-derived factor-1 (CXCL-12) was critical for the new method.

Results: More allogeneic HSCs homed into spleen and bone marrow for the new method as compared to each control group. IBBMT of endosteal BMCs led to a higher peripheral blood chimerism and skin allograft survival. At 18 weeks, donor stromal cell percentage in recipient BMCs was higher for the new method than in each control group. By AMD3100 blockade at day 1, peripheral blood chimerism level and donor stromal cell percentage were significantly reduced as compared to the control group without AMD3100 blockade.

Conclusions: Our study suggests that IBBMT of endosteal BMCs is an effective approach for HSCT in inducing allogeneic hematopoietic reconstitution. The advantage is dependent upon the early expression of CXCL-12 after bone marrow transplantation.

No MeSH data available.


Related in: MedlinePlus