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Infants born large-for-gestational-age display slower growth in early infancy, but no epigenetic changes at birth.

Chiavaroli V, Cutfield WS, Derraik JG, Pan Z, Ngo S, Sheppard A, Craigie S, Stone P, Sadler L, Ahlsson F - Sci Rep (2015)

Bottom Line: At birth, LGA infants had greater weight (p < 0.0001), length (p < 0.0001), ponderal index (p = 0.020), as well as greater head (p < 0.0001), chest (p = 0.044), and abdominal (p = 0.007) circumferences than AGA newborns.Genome-wide analysis showed no epigenetic differences between LGA and AGA infants.In addition, differences between AGA and LGA newborns were not associated with epigenetic changes.

View Article: PubMed Central - PubMed

Affiliation: Liggins Institute, University of Auckland, Auckland, New Zealand.

ABSTRACT
We evaluated the growth patterns of infants born large-for-gestational-age (LGA) from birth to age 1 year compared to those born appropriate-for-gestational-age (AGA). In addition, we investigated possible epigenetic changes associated with being born LGA. Seventy-one newborns were classified by birth weight as AGA (10(th)-90(th) percentile; n = 42) or LGA (>90(th) percentile; n = 29). Post-natal follow-up until age 1 year was performed with clinical assessments at 3, 6, and 12 months. Genome-wide DNA methylation was analysed on umbilical tissue in 19 AGA and 27 LGA infants. At birth, LGA infants had greater weight (p < 0.0001), length (p < 0.0001), ponderal index (p = 0.020), as well as greater head (p < 0.0001), chest (p = 0.044), and abdominal (p = 0.007) circumferences than AGA newborns. LGA infants were still larger at the age of 3 months, but by age 6 months there were no more differences between groups, due to higher length and weight increments in AGA infants between 0 and 6 months (p < 0.0001 and p = 0.002, respectively). Genome-wide analysis showed no epigenetic differences between LGA and AGA infants. Overall, LGA infants had slower growth in early infancy, being anthropometrically similar to AGA infants by 6 months of age. In addition, differences between AGA and LGA newborns were not associated with epigenetic changes.

No MeSH data available.


Related in: MedlinePlus

Graphic output of the CpGassoc method, showing the Manhattan plot for the association between methylation and AGA-LGA group.X-axis: location of CpG site in the genome by chromosome; y-axis: -log10 of the p-value for each CpG site (dots), with more negative values indicating greater differences between groups. The red horizontal line at the top of the figure represents the cutoff for FDR-adjusted p < 0.05; the absence of dots above this line shows that no statistically significant differences were observed.
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f2: Graphic output of the CpGassoc method, showing the Manhattan plot for the association between methylation and AGA-LGA group.X-axis: location of CpG site in the genome by chromosome; y-axis: -log10 of the p-value for each CpG site (dots), with more negative values indicating greater differences between groups. The red horizontal line at the top of the figure represents the cutoff for FDR-adjusted p < 0.05; the absence of dots above this line shows that no statistically significant differences were observed.

Mentions: The genome-wide methylation analysis was carried out on samples from 46 infants, including 19 AGA and 27 LGA infants. For this analysis, more than 485,000 DNA methylation sites covering 99% of human NCBI Reference Sequence (RefSeq) genes were examined at birth. 449,691 probes (92.6%) out of 485,577 passed the probe filtering criteria. The differential methylation analysis of CpGs sites showed no significant differences between LGA and AGA infants at birth (all p > 0.05) (Fig. 2 and Supplementary Figure 2).


Infants born large-for-gestational-age display slower growth in early infancy, but no epigenetic changes at birth.

Chiavaroli V, Cutfield WS, Derraik JG, Pan Z, Ngo S, Sheppard A, Craigie S, Stone P, Sadler L, Ahlsson F - Sci Rep (2015)

Graphic output of the CpGassoc method, showing the Manhattan plot for the association between methylation and AGA-LGA group.X-axis: location of CpG site in the genome by chromosome; y-axis: -log10 of the p-value for each CpG site (dots), with more negative values indicating greater differences between groups. The red horizontal line at the top of the figure represents the cutoff for FDR-adjusted p < 0.05; the absence of dots above this line shows that no statistically significant differences were observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588582&req=5

f2: Graphic output of the CpGassoc method, showing the Manhattan plot for the association between methylation and AGA-LGA group.X-axis: location of CpG site in the genome by chromosome; y-axis: -log10 of the p-value for each CpG site (dots), with more negative values indicating greater differences between groups. The red horizontal line at the top of the figure represents the cutoff for FDR-adjusted p < 0.05; the absence of dots above this line shows that no statistically significant differences were observed.
Mentions: The genome-wide methylation analysis was carried out on samples from 46 infants, including 19 AGA and 27 LGA infants. For this analysis, more than 485,000 DNA methylation sites covering 99% of human NCBI Reference Sequence (RefSeq) genes were examined at birth. 449,691 probes (92.6%) out of 485,577 passed the probe filtering criteria. The differential methylation analysis of CpGs sites showed no significant differences between LGA and AGA infants at birth (all p > 0.05) (Fig. 2 and Supplementary Figure 2).

Bottom Line: At birth, LGA infants had greater weight (p < 0.0001), length (p < 0.0001), ponderal index (p = 0.020), as well as greater head (p < 0.0001), chest (p = 0.044), and abdominal (p = 0.007) circumferences than AGA newborns.Genome-wide analysis showed no epigenetic differences between LGA and AGA infants.In addition, differences between AGA and LGA newborns were not associated with epigenetic changes.

View Article: PubMed Central - PubMed

Affiliation: Liggins Institute, University of Auckland, Auckland, New Zealand.

ABSTRACT
We evaluated the growth patterns of infants born large-for-gestational-age (LGA) from birth to age 1 year compared to those born appropriate-for-gestational-age (AGA). In addition, we investigated possible epigenetic changes associated with being born LGA. Seventy-one newborns were classified by birth weight as AGA (10(th)-90(th) percentile; n = 42) or LGA (>90(th) percentile; n = 29). Post-natal follow-up until age 1 year was performed with clinical assessments at 3, 6, and 12 months. Genome-wide DNA methylation was analysed on umbilical tissue in 19 AGA and 27 LGA infants. At birth, LGA infants had greater weight (p < 0.0001), length (p < 0.0001), ponderal index (p = 0.020), as well as greater head (p < 0.0001), chest (p = 0.044), and abdominal (p = 0.007) circumferences than AGA newborns. LGA infants were still larger at the age of 3 months, but by age 6 months there were no more differences between groups, due to higher length and weight increments in AGA infants between 0 and 6 months (p < 0.0001 and p = 0.002, respectively). Genome-wide analysis showed no epigenetic differences between LGA and AGA infants. Overall, LGA infants had slower growth in early infancy, being anthropometrically similar to AGA infants by 6 months of age. In addition, differences between AGA and LGA newborns were not associated with epigenetic changes.

No MeSH data available.


Related in: MedlinePlus