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Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.

Tanabe K, Kitagawa E, Wada M, Haraguchi A, Orihara K, Tahara Y, Nakao A, Shibata S - Sci Rep (2015)

Bottom Line: The light period group showed higher allergic diarrhea and weight loss than the dark period group.The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group.Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

ABSTRACT
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

No MeSH data available.


Related in: MedlinePlus

Tight junction (TJ) mRNA expression levels of Occludin (Ocln) and Claudin 3 (Cldn3).Jejunum tissues were collected from BALB/c mice that were sensitized but were not given any oral challenge with ovalbumin in the light period or the dark period. The mRNA expression levels of the TJ proteins Ocln and Cldn3 were measured using real time RT-PCR. The data are presented as means ± SEM (n = 5). *p < 0.05 light period group versus dark period group, calculated with a t-test.
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f4: Tight junction (TJ) mRNA expression levels of Occludin (Ocln) and Claudin 3 (Cldn3).Jejunum tissues were collected from BALB/c mice that were sensitized but were not given any oral challenge with ovalbumin in the light period or the dark period. The mRNA expression levels of the TJ proteins Ocln and Cldn3 were measured using real time RT-PCR. The data are presented as means ± SEM (n = 5). *p < 0.05 light period group versus dark period group, calculated with a t-test.

Mentions: We investigated whether the intestinal permeability in the mice was different in the light period and the dark period. We quantified the mRNA expression levels of TJ proteins, Ocln and Claudin 3 (Cldn3), in the jejunum. The mRNA expression levels of these genes were significantly lower in the light period group than the dark period group (Fig. 4). This result suggests that the intestinal permeability may be higher in the light period than the dark period.


Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.

Tanabe K, Kitagawa E, Wada M, Haraguchi A, Orihara K, Tahara Y, Nakao A, Shibata S - Sci Rep (2015)

Tight junction (TJ) mRNA expression levels of Occludin (Ocln) and Claudin 3 (Cldn3).Jejunum tissues were collected from BALB/c mice that were sensitized but were not given any oral challenge with ovalbumin in the light period or the dark period. The mRNA expression levels of the TJ proteins Ocln and Cldn3 were measured using real time RT-PCR. The data are presented as means ± SEM (n = 5). *p < 0.05 light period group versus dark period group, calculated with a t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588575&req=5

f4: Tight junction (TJ) mRNA expression levels of Occludin (Ocln) and Claudin 3 (Cldn3).Jejunum tissues were collected from BALB/c mice that were sensitized but were not given any oral challenge with ovalbumin in the light period or the dark period. The mRNA expression levels of the TJ proteins Ocln and Cldn3 were measured using real time RT-PCR. The data are presented as means ± SEM (n = 5). *p < 0.05 light period group versus dark period group, calculated with a t-test.
Mentions: We investigated whether the intestinal permeability in the mice was different in the light period and the dark period. We quantified the mRNA expression levels of TJ proteins, Ocln and Claudin 3 (Cldn3), in the jejunum. The mRNA expression levels of these genes were significantly lower in the light period group than the dark period group (Fig. 4). This result suggests that the intestinal permeability may be higher in the light period than the dark period.

Bottom Line: The light period group showed higher allergic diarrhea and weight loss than the dark period group.The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group.Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

ABSTRACT
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

No MeSH data available.


Related in: MedlinePlus