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Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.

Tanabe K, Kitagawa E, Wada M, Haraguchi A, Orihara K, Tahara Y, Nakao A, Shibata S - Sci Rep (2015)

Bottom Line: The light period group showed higher allergic diarrhea and weight loss than the dark period group.The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group.Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

ABSTRACT
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

No MeSH data available.


Related in: MedlinePlus

A higher concentration of ovalbumin (OVA) was absorbed into the blood in the light period treatment group than the dark period group.Non-sensitized mice were administrated OVA orally (80 mg/500 μl or 5 mg/300 μl distilled water) in the light period or in the dark period. Blood samples were collected after 30 min. The serum OVA level was measured by ELISA. The data are presented as means ± SEM. (n = 5–6). **p < 0.01 light period treatment versus dark period treatment, calculated with a Mann-Whitney test.
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f3: A higher concentration of ovalbumin (OVA) was absorbed into the blood in the light period treatment group than the dark period group.Non-sensitized mice were administrated OVA orally (80 mg/500 μl or 5 mg/300 μl distilled water) in the light period or in the dark period. Blood samples were collected after 30 min. The serum OVA level was measured by ELISA. The data are presented as means ± SEM. (n = 5–6). **p < 0.01 light period treatment versus dark period treatment, calculated with a Mann-Whitney test.

Mentions: We found a significant difference between the food allergic diarrhea induced in the light period and that in the dark period. We therefore hypothesized that OVA absorption is different in the light period and the dark period. In the body, the proteins that are absorbed from the intestinal lumen migrate to the blood stream. Thus, to determine OVA absorption, we measured the OVA concentration in the blood following oral OVA administration. Blood samples were collected 30 minutes after OVA administration in the light period or in the dark period. It has been well demonstrated that the OVA concentration in the blood reaches peak levels 20–30 minutes after oral administration28. We found that there were significant differences in the serum OVA levels at both oral doses used. Higher concentrations of OVA were absorbed in the light period group than the dark period group (Fig. 3).


Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.

Tanabe K, Kitagawa E, Wada M, Haraguchi A, Orihara K, Tahara Y, Nakao A, Shibata S - Sci Rep (2015)

A higher concentration of ovalbumin (OVA) was absorbed into the blood in the light period treatment group than the dark period group.Non-sensitized mice were administrated OVA orally (80 mg/500 μl or 5 mg/300 μl distilled water) in the light period or in the dark period. Blood samples were collected after 30 min. The serum OVA level was measured by ELISA. The data are presented as means ± SEM. (n = 5–6). **p < 0.01 light period treatment versus dark period treatment, calculated with a Mann-Whitney test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588575&req=5

f3: A higher concentration of ovalbumin (OVA) was absorbed into the blood in the light period treatment group than the dark period group.Non-sensitized mice were administrated OVA orally (80 mg/500 μl or 5 mg/300 μl distilled water) in the light period or in the dark period. Blood samples were collected after 30 min. The serum OVA level was measured by ELISA. The data are presented as means ± SEM. (n = 5–6). **p < 0.01 light period treatment versus dark period treatment, calculated with a Mann-Whitney test.
Mentions: We found a significant difference between the food allergic diarrhea induced in the light period and that in the dark period. We therefore hypothesized that OVA absorption is different in the light period and the dark period. In the body, the proteins that are absorbed from the intestinal lumen migrate to the blood stream. Thus, to determine OVA absorption, we measured the OVA concentration in the blood following oral OVA administration. Blood samples were collected 30 minutes after OVA administration in the light period or in the dark period. It has been well demonstrated that the OVA concentration in the blood reaches peak levels 20–30 minutes after oral administration28. We found that there were significant differences in the serum OVA levels at both oral doses used. Higher concentrations of OVA were absorbed in the light period group than the dark period group (Fig. 3).

Bottom Line: The light period group showed higher allergic diarrhea and weight loss than the dark period group.The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group.Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

ABSTRACT
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

No MeSH data available.


Related in: MedlinePlus