Limits...
Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.

Tanabe K, Kitagawa E, Wada M, Haraguchi A, Orihara K, Tahara Y, Nakao A, Shibata S - Sci Rep (2015)

Bottom Line: The light period group showed higher allergic diarrhea and weight loss than the dark period group.The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group.Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

ABSTRACT
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

No MeSH data available.


Related in: MedlinePlus

Mesenteric lymph node (MLN) cells from the light period ovalbumin (OVA) treated group produced higher IL-13 and IL-5 levels than the dark period treated group.After the second treatment, MLN cells were isolated and incubated with 1000 or 3000 μg of OVA. The type 2 cytokines IL-13 and IL-5 were measured in the culture medium by ELISA. The data are presented as means ± SEM. (n = 8). *p < 0.05 for light period treatment versus dark period treatment, calculated with a Mann-Whitney test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4588575&req=5

f2: Mesenteric lymph node (MLN) cells from the light period ovalbumin (OVA) treated group produced higher IL-13 and IL-5 levels than the dark period treated group.After the second treatment, MLN cells were isolated and incubated with 1000 or 3000 μg of OVA. The type 2 cytokines IL-13 and IL-5 were measured in the culture medium by ELISA. The data are presented as means ± SEM. (n = 8). *p < 0.05 for light period treatment versus dark period treatment, calculated with a Mann-Whitney test.

Mentions: We investigated the response of immune cells that were stimulated at different time points with OVA antigen. After the second challenge, MLN cells were harvested and stimulated with OVA in vitro. After three days, the culture supernatants were collected and type 2 cytokine production levels were measured. Cells obtained from the light period group produced significantly higher levels of IL-13 than the dark period group (Fig. 2). Although the data for IL-4 were below the detection limit, similar results of differential production level were obtained for IL-5 (Fig. 2). These results demonstrate that antigen stimulation in the light period, as opposed to the dark period, induces higher IL-13 and IL-5 production.


Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.

Tanabe K, Kitagawa E, Wada M, Haraguchi A, Orihara K, Tahara Y, Nakao A, Shibata S - Sci Rep (2015)

Mesenteric lymph node (MLN) cells from the light period ovalbumin (OVA) treated group produced higher IL-13 and IL-5 levels than the dark period treated group.After the second treatment, MLN cells were isolated and incubated with 1000 or 3000 μg of OVA. The type 2 cytokines IL-13 and IL-5 were measured in the culture medium by ELISA. The data are presented as means ± SEM. (n = 8). *p < 0.05 for light period treatment versus dark period treatment, calculated with a Mann-Whitney test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588575&req=5

f2: Mesenteric lymph node (MLN) cells from the light period ovalbumin (OVA) treated group produced higher IL-13 and IL-5 levels than the dark period treated group.After the second treatment, MLN cells were isolated and incubated with 1000 or 3000 μg of OVA. The type 2 cytokines IL-13 and IL-5 were measured in the culture medium by ELISA. The data are presented as means ± SEM. (n = 8). *p < 0.05 for light period treatment versus dark period treatment, calculated with a Mann-Whitney test.
Mentions: We investigated the response of immune cells that were stimulated at different time points with OVA antigen. After the second challenge, MLN cells were harvested and stimulated with OVA in vitro. After three days, the culture supernatants were collected and type 2 cytokine production levels were measured. Cells obtained from the light period group produced significantly higher levels of IL-13 than the dark period group (Fig. 2). Although the data for IL-4 were below the detection limit, similar results of differential production level were obtained for IL-5 (Fig. 2). These results demonstrate that antigen stimulation in the light period, as opposed to the dark period, induces higher IL-13 and IL-5 production.

Bottom Line: The light period group showed higher allergic diarrhea and weight loss than the dark period group.The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group.Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

ABSTRACT
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

No MeSH data available.


Related in: MedlinePlus