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Anthelmintic activity of trans-cinnamaldehyde and A- and B-type proanthocyanidins derived from cinnamon (Cinnamomum verum).

Williams AR, Ramsay A, Hansen TV, Ropiak HM, Mejer H, Nejsum P, Mueller-Harvey I, Thamsborg SM - Sci Rep (2015)

Bottom Line: Cinnamon (Cinnamomum verum) has been shown to have anti-inflammatory and antimicrobial properties, but effects on parasitic worms of the intestine have not been investigated.It is proposed that the rapid absorption or metabolism of CA in vivo may prevent it from being present in sufficient concentrations in situ to exert efficacy.Therefore, further work should focus on whether formulation of CA can enhance its activity against internal parasites.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.

ABSTRACT
Cinnamon (Cinnamomum verum) has been shown to have anti-inflammatory and antimicrobial properties, but effects on parasitic worms of the intestine have not been investigated. Here, extracts of cinnamon bark were shown to have potent in vitro anthelmintic properties against the swine nematode Ascaris suum. Analysis of the extract revealed high concentrations of proanthocyanidins (PAC) and trans-cinnamaldehyde (CA). The PAC were subjected to thiolysis and HPLC-MS analysis which demonstrated that they were exclusively procyanidins, had a mean degree of polymerization of 5.2 and 21% of their inter-flavan-3-ol links were A-type linkages. Purification of the PAC revealed that whilst they had activity against A. suum, most of the potency of the extract derived from CA. Trichuris suis and Oesophagostomum dentatum larvae were similarly susceptible to CA. To test whether CA could reduce A. suum infection in pigs in vivo, CA was administered daily in the diet or as a targeted, encapsulated dose. However, infection was not significantly reduced. It is proposed that the rapid absorption or metabolism of CA in vivo may prevent it from being present in sufficient concentrations in situ to exert efficacy. Therefore, further work should focus on whether formulation of CA can enhance its activity against internal parasites.

No MeSH data available.


Related in: MedlinePlus

Schematic experimental design.Stepwise outline of the anthelmintic testing of cinnamon bark extract, derived proanthocyanidin fractions and pure trans-cinnamaldehyde.
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f8: Schematic experimental design.Stepwise outline of the anthelmintic testing of cinnamon bark extract, derived proanthocyanidin fractions and pure trans-cinnamaldehyde.

Mentions: A schematic outline of the experimental design for the anthelmintic testing is presented in Fig. 8. Cinnamon bark extract was first extracted and tested against A. suum in vitro using migration and motility inhibition assays. Two PAC fractions were derived from the extract and also tested against A. suum in vitro. Pure CA was then purchased commercially and tested against A. suum, O. dentatum and T. suis larvae in vitro and A. suum larvae in vivo.


Anthelmintic activity of trans-cinnamaldehyde and A- and B-type proanthocyanidins derived from cinnamon (Cinnamomum verum).

Williams AR, Ramsay A, Hansen TV, Ropiak HM, Mejer H, Nejsum P, Mueller-Harvey I, Thamsborg SM - Sci Rep (2015)

Schematic experimental design.Stepwise outline of the anthelmintic testing of cinnamon bark extract, derived proanthocyanidin fractions and pure trans-cinnamaldehyde.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4588565&req=5

f8: Schematic experimental design.Stepwise outline of the anthelmintic testing of cinnamon bark extract, derived proanthocyanidin fractions and pure trans-cinnamaldehyde.
Mentions: A schematic outline of the experimental design for the anthelmintic testing is presented in Fig. 8. Cinnamon bark extract was first extracted and tested against A. suum in vitro using migration and motility inhibition assays. Two PAC fractions were derived from the extract and also tested against A. suum in vitro. Pure CA was then purchased commercially and tested against A. suum, O. dentatum and T. suis larvae in vitro and A. suum larvae in vivo.

Bottom Line: Cinnamon (Cinnamomum verum) has been shown to have anti-inflammatory and antimicrobial properties, but effects on parasitic worms of the intestine have not been investigated.It is proposed that the rapid absorption or metabolism of CA in vivo may prevent it from being present in sufficient concentrations in situ to exert efficacy.Therefore, further work should focus on whether formulation of CA can enhance its activity against internal parasites.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.

ABSTRACT
Cinnamon (Cinnamomum verum) has been shown to have anti-inflammatory and antimicrobial properties, but effects on parasitic worms of the intestine have not been investigated. Here, extracts of cinnamon bark were shown to have potent in vitro anthelmintic properties against the swine nematode Ascaris suum. Analysis of the extract revealed high concentrations of proanthocyanidins (PAC) and trans-cinnamaldehyde (CA). The PAC were subjected to thiolysis and HPLC-MS analysis which demonstrated that they were exclusively procyanidins, had a mean degree of polymerization of 5.2 and 21% of their inter-flavan-3-ol links were A-type linkages. Purification of the PAC revealed that whilst they had activity against A. suum, most of the potency of the extract derived from CA. Trichuris suis and Oesophagostomum dentatum larvae were similarly susceptible to CA. To test whether CA could reduce A. suum infection in pigs in vivo, CA was administered daily in the diet or as a targeted, encapsulated dose. However, infection was not significantly reduced. It is proposed that the rapid absorption or metabolism of CA in vivo may prevent it from being present in sufficient concentrations in situ to exert efficacy. Therefore, further work should focus on whether formulation of CA can enhance its activity against internal parasites.

No MeSH data available.


Related in: MedlinePlus