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Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H - Sci Rep (2015)

Bottom Line: Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06.Significant increase in photosynthetic pigment with PEG priming under stress.Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.

No MeSH data available.


Related in: MedlinePlus

Electron micrographs of leaf mesophyll and root cell of two cultivars of Oryza sativa (cvs. Zhu Liang You 06 and Qian You No. 1) primed with PEG (30%) and grow under control and 750 mg L−1 of nano-ZnO concentration.(A) Leaf mesophyll cell of cultivar Zhu Liang You 06 at control level. (B) Leaf mesophyll cell of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (C) Leaf cell of cultivar Zhu Liang You 06 non-primed with PEG and exposed to 750 mg L−1. (D) Leaf mesophyll cell of cultivar Qian You No. 1 under control. (E) Leaf mesophyll cell of Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (F) Leaf mesophyll cell of Qian You No. 1 non-primed with PEG (30%) and exposed to 750 mg L−1. (G) Root tip of cultivar Zhu Liang You 06 under control. (H) Root tip of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (I) Root tip of cultivar Zhu Liang You 06 non-primed with PEG (30%) and exposed to 750 mg L−1. (J) Root tip of cultivar Qian You No. 1 under control. (K) Root tip of cultivar Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (L) Root tip of cultivar Qian You No. 1 non-primed with PEG and exposed to 750 mg L−1.
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f4: Electron micrographs of leaf mesophyll and root cell of two cultivars of Oryza sativa (cvs. Zhu Liang You 06 and Qian You No. 1) primed with PEG (30%) and grow under control and 750 mg L−1 of nano-ZnO concentration.(A) Leaf mesophyll cell of cultivar Zhu Liang You 06 at control level. (B) Leaf mesophyll cell of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (C) Leaf cell of cultivar Zhu Liang You 06 non-primed with PEG and exposed to 750 mg L−1. (D) Leaf mesophyll cell of cultivar Qian You No. 1 under control. (E) Leaf mesophyll cell of Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (F) Leaf mesophyll cell of Qian You No. 1 non-primed with PEG (30%) and exposed to 750 mg L−1. (G) Root tip of cultivar Zhu Liang You 06 under control. (H) Root tip of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (I) Root tip of cultivar Zhu Liang You 06 non-primed with PEG (30%) and exposed to 750 mg L−1. (J) Root tip of cultivar Qian You No. 1 under control. (K) Root tip of cultivar Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (L) Root tip of cultivar Qian You No. 1 non-primed with PEG and exposed to 750 mg L−1.

Mentions: The ultrastructural changes in leaf mesophyll and root tip cells under control and higher nano-ZnO concentration (750 mg L−1) have been illustrated in Fig. 4. The TEM micrographs of leaf cell of cultivar Zhu Liang You 06 at control showed clean and thin cell walls, well-developed chloroplast with granule thylakoid, and the cell with rich contents and normal organelles (Fig. 4A). While, The TEM micrographs of leaf mesophyll of cultivar Zhu Liang You 06 (unprimed with PEG and exposed to 750 mg L−1) are displayed in Fig. 4C. It was found that this concentration destructed the cell wall and untidy arrangement of the thylakoid inside the chloroplast as compared to their respective control. Whereas, priming with PEG reduced the stress effect represented in clear cell wall (CW), well developed chloroplast (Ch) and tidy granule thylakoid inside the chloroplast (Fig. 4B), as well as no significant changes were found in the leaf mesophyll cell among two cultivars.


Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H - Sci Rep (2015)

Electron micrographs of leaf mesophyll and root cell of two cultivars of Oryza sativa (cvs. Zhu Liang You 06 and Qian You No. 1) primed with PEG (30%) and grow under control and 750 mg L−1 of nano-ZnO concentration.(A) Leaf mesophyll cell of cultivar Zhu Liang You 06 at control level. (B) Leaf mesophyll cell of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (C) Leaf cell of cultivar Zhu Liang You 06 non-primed with PEG and exposed to 750 mg L−1. (D) Leaf mesophyll cell of cultivar Qian You No. 1 under control. (E) Leaf mesophyll cell of Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (F) Leaf mesophyll cell of Qian You No. 1 non-primed with PEG (30%) and exposed to 750 mg L−1. (G) Root tip of cultivar Zhu Liang You 06 under control. (H) Root tip of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (I) Root tip of cultivar Zhu Liang You 06 non-primed with PEG (30%) and exposed to 750 mg L−1. (J) Root tip of cultivar Qian You No. 1 under control. (K) Root tip of cultivar Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (L) Root tip of cultivar Qian You No. 1 non-primed with PEG and exposed to 750 mg L−1.
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Related In: Results  -  Collection

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f4: Electron micrographs of leaf mesophyll and root cell of two cultivars of Oryza sativa (cvs. Zhu Liang You 06 and Qian You No. 1) primed with PEG (30%) and grow under control and 750 mg L−1 of nano-ZnO concentration.(A) Leaf mesophyll cell of cultivar Zhu Liang You 06 at control level. (B) Leaf mesophyll cell of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (C) Leaf cell of cultivar Zhu Liang You 06 non-primed with PEG and exposed to 750 mg L−1. (D) Leaf mesophyll cell of cultivar Qian You No. 1 under control. (E) Leaf mesophyll cell of Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (F) Leaf mesophyll cell of Qian You No. 1 non-primed with PEG (30%) and exposed to 750 mg L−1. (G) Root tip of cultivar Zhu Liang You 06 under control. (H) Root tip of cultivar Zhu Liang You 06 primed with PEG (30%) and exposed to 750 mg L−1. (I) Root tip of cultivar Zhu Liang You 06 non-primed with PEG (30%) and exposed to 750 mg L−1. (J) Root tip of cultivar Qian You No. 1 under control. (K) Root tip of cultivar Qian You No. 1 primed with PEG (30%) and exposed to 750 mg L−1. (L) Root tip of cultivar Qian You No. 1 non-primed with PEG and exposed to 750 mg L−1.
Mentions: The ultrastructural changes in leaf mesophyll and root tip cells under control and higher nano-ZnO concentration (750 mg L−1) have been illustrated in Fig. 4. The TEM micrographs of leaf cell of cultivar Zhu Liang You 06 at control showed clean and thin cell walls, well-developed chloroplast with granule thylakoid, and the cell with rich contents and normal organelles (Fig. 4A). While, The TEM micrographs of leaf mesophyll of cultivar Zhu Liang You 06 (unprimed with PEG and exposed to 750 mg L−1) are displayed in Fig. 4C. It was found that this concentration destructed the cell wall and untidy arrangement of the thylakoid inside the chloroplast as compared to their respective control. Whereas, priming with PEG reduced the stress effect represented in clear cell wall (CW), well developed chloroplast (Ch) and tidy granule thylakoid inside the chloroplast (Fig. 4B), as well as no significant changes were found in the leaf mesophyll cell among two cultivars.

Bottom Line: Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06.Significant increase in photosynthetic pigment with PEG priming under stress.Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.

No MeSH data available.


Related in: MedlinePlus